Jerzy Rojek  Aleksander Zubelewicz  Nikhil Madan  Szymon Nosewicz 《International journal for numerical methods in engineering》2018,114(8):828-860

This work presents a new original formulation of the discrete element method (DEM) with deformable cylindrical particles. Uniform stress and strain fields are assumed to be induced in the particles under the action of contact forces. Particle deformation obtained by strain integration is taken into account in the evaluation of interparticle contact forces. The deformability of a particle yields a nonlocal contact model, it leads to the formation of new contacts, it changes the distribution of contact forces in the particle assembly, and it affects the macroscopic response of the particulate material. A numerical algorithm for the deformable DEM (DDEM) has been developed and implemented in the DEM program DEMPack. The new formulation implies only small modifications of the standard DEM algorithm. The DDEM algorithm has been verified on simple examples of an unconfined uniaxial compression of a rectangular specimen discretized with regularly spaced equal bonded particles and a square specimen represented with an irregular configuration of nonuniform‐sized bonded particles. The numerical results have been verified by a comparison with equivalent finite element method results and available analytical solutions. The micro‐macro relationships for elastic parameters have been obtained. The results have proved to have enhanced the modeling capabilities of the DDEM with respect to the standard DEM.  相似文献   

    

Szymon Bobek  Grzegorz J. Nalepa  Mateusz lay&#x;ski 《Expert Systems》2019,36(1)

Building mobile context‐aware systems is inherently complex and non‐trivial task. It consists of several phases starting from acquisition of context, through modeling to execution of contextual models. Today, such systems are mostly implemented on mobile platforms, that introduce specific requirements, such as intelligibility, robustness, privacy, and efficiency. Over the last decade, along with the rapid development of mobile industry, many approaches were developed that unevenly support these requirements. This is mainly caused by the fact that current modelling and reasoning methods are not crafted to operate in mobile environments. We argue that the use of rule‐based reasoning tailored to mobile environments is an optimal solution. Rules are based on symbolic knowledge representation, as such they meet the general tendency to enforce understandability, intelligibility, and controllability of artificial intelligence software, as stated in the recent European Union General Data Protection Regulation. To this goal, we introduce a lightweight rule engine dedicated for Android platform called HEARTDROID. It executes models in the HMR+ rule language that are capable of expressing uncertainty of knowledge, capturing dynamics of mobile environment and provide high level of intelligibility. We present a qualitative and quantitative comparison of HEARTDROID with the most popular rule engines available.  相似文献   

    

Nikhil Madan  Jerzy Rojek  Szymon Nosewicz 《International journal for numerical methods in engineering》2019,118(6):320-344

This work investigates numerical properties of the algorithm of the discrete element method (DEM) employing deformable circular disks presented in the authors' earlier publication. The new formulation called the deformable DEM (DDEM) enhances the standard DEM (SDEM) by introducing an additional (global) deformation mode caused by the stresses in the particles induced by the contact forces. An accurate computation of the contact forces would require an iterative solution of the implicit relationship between the contact forces and particle displacements. In order to preserve efficiency of the DEM, the new formulation has been adapted to the explicit time integration. It has been shown that the explicit DDEM algorithm is conditionally stable and there are two restrictions on its stability. Except for the limitation of the time step as in the SDEM, the stability in the DDEM is governed by the convergence criterion of the iterative solution of the contact forces. The convergence and stability limits have been determined analytically and numerically for selected regular and irregular configurations. It has also been found out that the critical time step in DDEM remains unchanged with respect to the SDEM.  相似文献   

    

Bart&#x;omiej Pawlik  Szymon Grabia  Urszula Smyczy&#x;ska  Wojciech Fendler  Izabela Drd  Ewa Liszewska  Jacek Jaworski  Katarzyna Kotulska  Sergiusz J wiak  Wojciech M&#x;ynarski  Joanna Treli&#x;ska 《International journal of molecular sciences》2022,23(22)

The aim of this study was to assess the potential implication of microRNA on tuberous sclerosis (TSC) pathogenesis by performing microRNA profiling on cell lines silencing TSC1 or TSC2 genes using qPCR panels, before and after incubation with rapamycin. Significant differences in expression were observed between samples before and after rapamycin treatment in nineteen miRNAs in TSC1, five miRNAs in TSC2 and seven miRNAs in controls. Of miRNAs dysregulated before rapamycin treatment, three normalized after treatment in the TSC1 group (miR-21-3p, miR-433-3p, let-7g-3p) and one normalized in the TSC2 group (miR-1224-3p). Of the miRNAs dysregulated before rapamycin treatment in the TSC1 and TSC2 groups, two did not normalize after treatment (miR-33a-3p, miR-29a-3p). The results of the possible targets indicated that there are four common genes with seed regions susceptible to regulation by those miRNAs: ZBTB20, PHACTR2, PLXNC1 and ATP1B4. Our data show no changes in mRNA expression of these targets after rapamycin treatment. In conclusion, results of our study indicate the involvement of miRNA dysregulation in the pathogenesis of TSC. Some of the miRNA might be used as markers of treatment efficacy and autonomic miRNA as a target for future therapy.  相似文献   

    

Adrianna Banach-Kope&#x;  Szymon Mania  Joanna Pilch  Ewa Augustin  Iwona Gabriel  Robert Tylingo 《International journal of molecular sciences》2022,23(10)

The article presents a new approach in the purification of chitosan (CS) hydrogel in order to remove a significant amount of endotoxins without changing its molecular weight and viscosity. Two variants of the method used to purify CS hydrogels from endotoxins were investigated using the PyroGene rFC Enzymatic Cascade assay kit. The effect of the CS purification method was assessed in terms of changes in the dynamic viscosity of its hydrogels, the molecular weight of the polymer, microbiological purity after refrigerated storage and cytotoxicity against L929 cells based on the ISO 10993-5:2009(E) standard. The proposed purification method 1 (M1) allows for the removal of significant amounts of endotoxins: 87.9–97.6% in relation to their initial concentration in the CS hydrogel without affecting the solution viscosity. Moreover, the final solutions were sterile and microbiologically stable during storage. The M1 purification method did not change the morphology of the L929 cells.  相似文献   

    

Adrian Chajec  Szymon Kwietniowski  Zuzanna Woźniak  Branko Šavija  Łukasz Sadowski 《Structural Concrete》2023,24(3):4064-4076

Bleeding is a common problem in concrete slabs, and may lead to serious damage. The goal of this article is to understand the impact of alternative binders and their properties on the bleeding of concrete. Therefore, the impact of the type of binder on the bleeding process is investigated. The results show that the addition of granite powder or fly ash allows for the increased control of the bleeding process. It was found that a finer particle size distribution (PSD), an increased specific surface area (SSA), and a higher bulk density may reduce the amount of dispensed water in the concrete mix. Furthermore, the use of additives with an increased SSA leads to even a 30% reduction of the bleeding rate of mixes. The utilization of additives with a finer PSD than cement enables a 37% reduction of the bleeding rate of mixes. The influence of bleeding on compressive strength was assessed using destructive and non-destructive tests: Replacing 30% of cement with granite powder leads to a 30% reduction of concrete strength after 28 days of curing; on the other hand, replacing 30% of cement with siliceous fly ash leads to an 18% reduction of strength. Importantly, bleeding was also found to lead to the heterogeneity of the physical and mechanical properties across the concrete section. Consequently, the proper control of the bleeding process leads to more homogeneous properties of concrete across its cross-section.  相似文献   

    

Dominika Czerwonka  Dr. Alicja Urbaniak  Szymon Sobczak  Dr. Sergio Piña-Oviedo  Dr. Timothy C. Chambers  Dr. Michał Antoszczak  Prof. Adam Huczyński 《ChemMedChem》2020,15(2):236-246

The polyether ionophore salinomycin ( SAL ) has captured much interest because of its potent activity against cancer cells and cancer stem cells. Our previous studies have indicated that C1/C20 double-modification of SAL is a useful strategy to generate diverse agents with promising biological activity profiles. Thus, herein we describe the synthesis of a new class of SAL analogues that combine key modifications at the C1 and C20 positions. The activity of the obtained SAL derivatives was evaluated using primary acute lymphoblastic leukemia, human breast adenocarcinoma and normal mammary epithelial cells. One single- [N,N-dipropyl amide of salinomycin ( 5 a )] and two novel double-modified analogues [N,N-dipropyl amide of C20-oxosalinomycin ( 5 b ) and piperazine amide of C20-oxosalinomycin ( 13 b )] were found to be more potent toward the MDA-MB-231 cell line than SAL or its C20-oxo analogue 2 . When select analogues were tested against the NCI-60 human tumor cell line panel, 4 a [N,N-diethyl amide of salinomycin] showed particular activity toward the ovarian cancer cell line SK-OV-3. Additionally, both SAL and 2 were found to be potent ex vivo against human ER/PR⁺, Her2⁻ invasive mammary carcinoma, with 2 showing minimal toxicity toward normal epithelial cells. The present findings highlight the therapeutic potential of SAL derivatives for select targeting of different cancer types.  相似文献   

    

Joanna Jonca  Malgorzata Waleron  Paulina Czaplewska  Aleksandra Bogucka  Aleksandra Ste&#x;  Szymon Dziomba  Jacek Jasiecki  Micha&#x; Rych&#x;owski  Krzysztof Waleron 《International journal of molecular sciences》2021,22(22)

Bacteria of genus Pectobacterium are Gram-negative rods of the family Pectobacteriaceae. They are the causative agent of soft rot diseases of crops and ornamental plants. However, their virulence mechanisms are not yet fully elucidated. Membrane vesicles (MVs) are universally released by bacteria and are believed to play an important role in the pathogenicity and survival of bacteria in the environment. Our study investigates the role of MVs in the virulence of Pectobacterium. The results indicate that the morphology and MVs production depend on growth medium composition. In polygalacturonic acid (PGA) supplemented media, Pectobacterium produces large MVs (100–300 nm) and small vesicles below 100 nm. Proteomic analyses revealed the presence of pectate degrading enzymes in the MVs. The pectate plate test and enzymatic assay proved that those enzymes are active and able to degrade pectates. What is more, the pathogenicity test indicated that the MVs derived from Pectobacterium were able to induce maceration of Zantedeschia sp. leaves. We also show that the MVs of β-lactamase producing strains were able to suppress ampicillin activity and permit the growth of susceptible bacteria. Those findings indicate that the MVs of Pectobacterium play an important role in host-pathogen interactions and niche competition with other bacteria. Our research also sheds some light on the mechanism of MVs production. We demonstrate that the MVs production in Pectobacterium strains, which overexpress a green fluorescence protein (GFP), is higher than in wild-type strains. Moreover, proteomic analysis revealed that the GFP was present in the MVs. Therefore, it is possible that protein sequestration into MVs might not be strictly limited to periplasmic proteins. Our research highlights the importance of MVs production as a mechanism of cargo delivery in Pectobacterium and an effective secretion system.  相似文献   

Propellane. VI. Synthese und Stereochemie des 8,11-Dioxa[3,4-naphtho-(ß,ß)]-[4.3.3]propellans     

Janusz Jamrozik  Maria Jamrozik  Szymon Schab  Iwona Strona 《Advanced Synthesis \u0026amp; Catalysis》1990,332(5):805-808

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Maciej Piejko  Szymon Niewieczerzal  Joanna I. Sulkowska 《Israel journal of chemistry》2020,60(7):713-724

The mechanism of deep knot formation in proteins has been debated for the past two decades, but definitive answers are still lacking. In this review, we first describe knotted proteins from the perspective of shallow and deep knots, taking into account recent experimental and theoretical results. We focus on the folding mechanism, where this difference is most profound. We explain in more details the cotranslational knotting pathway. Then, we additionally show that proteins with extremely deep knots have a distinct mechanism of knotting from proteins with shallow knots. The approach based on treating shallow and deep knots as separate classes of molecules allow to classify them better and introduce a new paradigm of thinking about knotted proteins as such. This may in turn help to avoid ambiguities in further research.  相似文献