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41.
Formation of dityrosine and other fluorescent amino acids by reaction of amino acids with lipid hydroperoxides 总被引:7,自引:0,他引:7
Formation of fluorescence by the reaction of various amino acids with lipid hydroperoxides,i.e., linoleic acid 13-monohydroperoxide, methyl linoleate 13-monohydroperoxide and phosphatidylcholine hydroperoxide, in the
presence of methemoglobin was investigated. Two types of fluorescence were produced: fluorescent dityrosine (3,3′-dityrosine)
from tyrosine, and unidentified fluorophores with α- and ε-amino groups of various amino acids. While the former was stable
after treatment with borohydride, the latter fluorophores were readily destroyed. The rate of dityrosine formation was rapid,
and the yield of dityrosine was dependent on the concentrations of tyrosine and the lipid hydroperoxides. Butylated hydroxytoluene
and tocopherol inhibited the formation of dityrosine, but did not affect the formation of fluorophores on the amino groups.
Dityrosine appears to be formed by radical reaction of the lipid hydroperoxides, while the other fluorophores seem to be created
by nonradical mechanisms. 相似文献
42.
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44.
Keiji Itabashi Takuji Ikeda Akihiko Matsumoto Kunikazu Kamioka Masanao Kato Kazuo Tsutsumi 《Microporous and mesoporous materials》2008,114(1-3):495-506
Four types of Rb-aluminosilicate zeolites were hydrothermally synthesized in pure phase for the first time from Rb-aluminosilicate gels without using any organic structure-directing agent (SDA) under stirring conditions. The crystal structure of each zeolite was refined by Rietveld analysis of the X-ray diffraction (XRD) data. These crystal structures were confirmed to be Rb-mordenite, Rb-merlinoite, a new aluminosilicate zeolite with an ATT framework topology, and Rb-offretite denoted by RMA-1, RMA-2, RMA-3, and RMA-4, respectively. The Si/Al ratio of RMA-1 with an MOR topology varied from 5.3 to 8.0; however, the variation of the Si/Al ratios of the other zeolites was rather small. The crystal system of RMA-2 was tetragonal with space group I4/mmm, where two Rb sites were distributed at the center of an 8-membered ring (MR). On the other hand, two Rb sites in RMA-3 were located at the center of the 8-MR in small two cages. The structure of RMA-4 was identified as the OFF type with a local disorder or defect, which included a small amount of an intergrown ERI phase. 相似文献
45.
T. Shimada M. Mizuno K. Kurachi N. Kato O. Sakurada M. Hashiba Y. Nurishi 《Journal of Materials Science》1996,31(14):3691-3695
Low thermal expansion coefficient and high mechanical strength have been attained simultaneously in a composite with microstructures in which the low thermal expansion grains are surrounded by a continuous texture of the grains with high strength. Alloying eucryptite with yttria-stabilized PSZ (partially stabilized zirconia) simultaneously achieved the low thermal expansion coefficient, 1.45 × 10–6, and the high bending strength, 220 MPa, by adjusting the composition and controlling the microstructures by changing the starting powders and the milling durations. Grain size and reactions between the coupled materials determined the critical processing conditions. 相似文献
46.
Serum rheumatoid factor (RF) level and peritoneal and splenic CD5+B (B-1) cells in mice were examined after intraperitoneal administration of purified lipopoly-saccharides (LPS) from oral periodontopathic bacteria; Porphyromonas gingivalis, Actinobacillus actinomycetemcomitans, Fusobacterium nucleatum and Capnocytophaga ochracea. F. nucleatum and C. ochracea LPS induced higher levels of serum IgM- and IgG-RF, while P. gingivalis LPS showed the least induction. In addition, wet weights of spleen and serum IgM and IgG concentration were markedly increased in F. nucleatum LPS injected group. On the other hand, the proportion of CD5+ B cells to lymphocytes in the peritoneal cavity and spleen did not increase. The reason for this was not clear but conventional B cells (CD5- B cells) might increase more rapidly with splenic enlargement than CD5+ B cells. These results suggested that RF induced by bacterial LPS may modulate immune responses against bacteria and plays an important role for defence and destruction of periodontal tissue. 相似文献
47.
48.
S Suzuki M Saito N Suzuki H Kato N Nagaoka S Yoshitake Y Yui C Kawai 《Canadian Metallurgical Quarterly》1993,22(6):834-840
We examined the thrombolytic properties of a novel modified human tissue plasminogen activator (PA) (E6010), in which cysteine 84 is replaced by serine, and which has a prolonged biologic half-life (t1/2). We compared the thrombolytic efficacy of continuous intracoronary (i.c.) infusion of E6010 with that of recombinant human tissue PA (rt-PA) in a canine model with copper coil-induced 1-h-old coronary artery thrombi and also compared the relation between thrombolytic efficacy and plasma clearance represented by pharmacokinetic parameters of i.c.-infused E6010 and rt-PA. Sixty-minute E6010 and rt-PA i.c. infusions were compared. The thrombolytic effects of i.c.-infused E6010 and rt-PA, represented by time to reperfusion (TR), reperfusion rate at 60 min (RR), and reocclusion rates at 60 min after reperfusion (OR) were as follows. E6010: Dose 0.06, 0.15, 0.3 (mg/kg/h); TR 25 +/- 10, 15 +/- 10, 13 +/- 5 (min); RR 100, 100, 100 (%); and OR 0, 0, 17 (%), respectively. Recombinant t-PA: Dose 0.06, 0.15, 0.3 (mg/kg/h); TR 47 +/- 12, 18 +/- 17, 14 +/- 4 (min); RR 50, 75, 100 (%); and OR 100, 33, 33 (%), respectively. These findings indicate that E6010 has more potent thrombolytic activity than rt-PA.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
49.
Chemical processing and characterization of spinel-type thermistor powder in the Mn-Ni-Fe oxide system 总被引:1,自引:0,他引:1
Y. Torii A. Tsuzuki K. Kato Y. Uwamino B. H. Choi M. J. Lee 《Journal of Materials Science》1996,31(10):2603-2607
The coprecipitated Mn-Ni-Fe oxalate was prepared from the mixed sulfate solution by optimum pH control. The chemical composition agreed well with that of the starting solution, and the thermal decomposition of the Mn-Ni-Fe oxalate in air below 600 °C led to the direct formation of cubic spinel phase with fine particle size. The sinterability of the calcined powder, the stability of cubic spinel phase and the influence of annealing temperature on electrical properties of the sintered bodies, were investigated. 相似文献
50.
We developed a noble technique for the fine-particle handling in plasmas. In this method a pair of point electrodes are introduced in the plasma, to which positive pulses are applied alternatively. When the pulse repetition period is shorter than the particle response time, the particles feel only time-averaged force because of their large mass. Spatial profile of the equivalent potential of the time-averaged force varies from a circle to an ellipse with an increase in the local discharge at the electrodes. The particles are eventually transported toward a middle point between two point-electrodes, being almost independent of their initial positions. This method is quite effective for converging fine particles in the plasma. 相似文献