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91.
92.
Arima J Uesugi Y Iwabuchi M Hatanaka T 《Protein engineering, design & selection : PEDS》2008,21(1):45-53
We purified and characterized the aminopeptidase P from Streptomyces costaricanus TH-4 (thAPP). This enzyme has a tetramer structure, a metal-ion preference toward Zn, broad substrate specificity and a narrow pH dependency for activity. The primary structure of thAPP, respectively, exhibits 91% and 65% identity with those of two other APPs-APP I and APP II-from Streptomyces lividans (slAPP I and slAPP II). We next overexpressed the genes encoding thAPP and slAPP II in Escherichia coli and characterized them. Two differences were apparent in their properties: slAPP II formed a dimer, whereas thAPP formed a tetramer; also, the alkaline side pKa for the catalytic action of slAPP II is higher than that of thAPP. Investigation using chimeras of both enzymes revealed that the N-terminal domain is associated with the determination of pKa values for catalytic action and quaternary structure. 相似文献
93.
A study was made to investigate an optimum condition for ruthenium tetraoxide staining of poly(butylene terephthalate) (PBT) with glass transition temperature as low as ca. 23 degrees C for TEM observation of melt-grown PBT crystals. The morphological study of ultrathin sections prepared based on optimum staining at 25 degrees C for 8 h revealed formation of fringed-micellar crystal nuclei in the early stage and folded-chain fringed-micellar crystals in the later stage of isothermal crystallization at 40 degrees C from melt. The fraction of chain-folded crystals increased with increasing crystallization temperature from the quantitative analysis of crystal thickness in comparison with the reported morphology using the replica method. 相似文献
94.
H Shimada K Nakajima T Ochiai Y Koide SI Okazumi H Matsubara A Takeda Y Miyazawa M Arima K Isono 《Canadian Metallurgical Quarterly》1998,5(4):871-874
The significance of serum p53-Abs in patients with esophageal squamous cell carcinoma was determined. Examination of clinicopathological features and assessment of tumor marker sensitivities of carcinoembryonic antigen (CEA), squamous cell carcinoma antigen (SCC-Ag) and CYFRA21-1 were performed. Thirty-three (58%) of 57 patients were positive for serum p53-Abs, however, no relation with cancer progression existed. Fourteen of the 33 sero-positive patients revealed normal levels of all tumor markers tested. Thus, serum p53-Abs appears to be a useful marker for the detection of esophageal squamous cell carcinoma. 相似文献
95.
Particle-free silicon and nickel thin films were successfully fabricated by laser-ablating a melted section of their target surface, which gives a high evaporation pressure at the melting point. The influence of direct evaporation from a melted target was reduced negligibly by melting the target only locally with a focused electron beam (e-beam) and increasing the laser frequency. The silicon films fabricated by the present method, pulse laser deposition of a partially molten target, were able to firmly adhere to the substrates and withstood steel needle scratching, unlike e-beam-evaporated films. 相似文献
96.
In this paper, we propose a learning algorithm for coordinating a robot system where the movement of an arm is controlled
through a stereo camera system. Instead of calibrating the usually complex non-linear transformation between the arm and cameras,
the algorithm decomposes the whole transformation automatically into local linear transformations and then makes the linearization
map recorded by the arm controller. The linearization is carried out by a learning process based on a Kohonen-style self-organization
network. To deal with unstructured environments in which some obstacles exist, some virtual forces are introduced for dealing
with the high degree of complexity underlying in the transformation.
This work was presented, in part, at the International Symposium on Artificial Life and Robotics, Oita, Japan, February 18–20,
1996 相似文献
97.
Self-organized rhodamine 6G particles prepared by wetting/dewetting process of an ethanol solution on a hydrophilic glass surface exhibits fluorescence without quenching, showing a sharp linewidth of 2nm with a large redshift, which indicates an existence of dye aggregates, similar to J-aggregates, inside the particle. Polarized evanescent field excitation showed that the dye molecule's transition moment along the pi-conjugation was oriented unidirectionally within particles and parallel to the substrate surface. This deduced dye orientation showed correlation between adjacent, but separated, particles and pointed roughly 45 degrees off the dewetting direction. In contrast, the particles of another pi-conjugated NK1420 dye, J-aggregates of which grows easily from an oversaturated solution, showed dye orientation along the dewetting direction preferably, still indicating the effect of self-organization, however based on a different mechanism. An annealing procedure revealed that both aggregates are in quasi-stable states, which is consistent with the rapidness of the dewetting process that may lead to crystallization in nonequilibrium. 相似文献
98.
Ryoichi Furushima Yohtaro Matsuo Tadashi Shiota Kouichi Yasuda 《Journal of Nondestructive Evaluation》2011,30(1):29-40
Changes in amplitude and phase shifts in plane elastic waves were theoretically estimated when they propagated through an
elastic body containing cylindrical inclusions. Based on the integral equation suggested by Lavrov et al., these changes were
calculated in the case when only one cylindrical inclusion was located in the body and the propagating waves have a single
frequency. These calculations were expanded to more practical case when multiple cylindrical inclusions aligned in the body
and the waves have some frequency band. The changes in amplitude and phase shifts calculated were associated to the changes
in apparent sonic velocity and attenuation coefficient due to a structural change in the material. This theoretical approach
contributes to a quantitative damage evaluation based upon structural changes in various materials by measuring the acoustical
parameters such as apparent sonic velocity and attenuation coefficient. 相似文献
99.
Shoichi Nasu Takaaki Tanifuji Katsuya Uchida Kenji Noda Toshimasa Kurasawa Tadashi Takahashi Masahiko Isshiki Takejiro Miyauchi Kohji Tanuma Fumio Sasajima 《Journal of Nuclear Materials》1980,91(1):121-126
The center temperature of Li2O pellets under neutron irradiation was measured using an encapsuled pin. The surface temperatures of the pellets and the pellet/cladding heat transfer coefficients were estimated on the basis of the well-known conductivity integral. 相似文献
100.
Michio Yamada Konomi Tokutomi Ayaka Takehara Yusuke Sakai Yutaka Maeda Tadashi Hasegawa 《Fullerenes, Nanotubes and Carbon Nanostructures》2014,22(1-3):66-74
Tröger's base (TB, 1) and its analogues were synthesized and were subject to the titration experiments to evaluate their ability of supramolecular complexation with C60. Results demonstrate clearly that the fluorene-based TB analogue (2) and the bioctylfluorene-based TB analogue (3) show 1:1 binding with C60 to form the corresponding complexes while TB 1 does not. Titration experiments exhibited that the association constant (Kass) of 3/C60 (48.1 ± 7.9 M ?1) was larger than that of 2/C60 (13.7 ± 1.4 M ?1). The formation of the 1:1 binding complexes 2/C60 and 3/C60 was also confirmed by MALDI-TOF mass spectrometry. Theoretical calculations suggest that 2 and 3 have appropriate cavities to embrace C60. These findings indicate that not only the π/π interaction between the fluorene moieties and the C60 surface but also the CH/π interaction between the octyl groups and the C60 surface serve efficiently in the supramolecular complexation with C60. 相似文献