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排序方式: 共有470条查询结果,搜索用时 15 毫秒
91.
Saowaluk Rungchang Sonthaya Numthuam Xiaoli Qiu Yanjie Li Takaaki Satake 《Journal of food engineering》2013
A variety of polyethylene terephthalate (PET) bottles commercially available as soft drink containers from 80 different brands was collected in Japan and subsequently examined to determine the amount of Sb in both the PET plastic and drink. The concentration of Sb in the plastic material ranged from 0.1 to 216.5 mg/kg, while this value ranged from 0.3 to 1.6 μg/L in bottled solutions. The diffusion coefficients of Sb in PET samples were determined at 25, 40, 55, and 70 °C. The migrated-Sb in all simulated food solutions (e.g., ultra pure water, 4% acetic acid, and 50% ethanol) increased with storage time and temperature. The temperature-dependent diffusion coefficients were derived from the Arrhenius equation. The predicted model for Sb migration suggested that storage conditions of all PET drink products should be below 70 °C for a maximum of 72 d to avoid Sb levels above the recommended value of 5 μg/L, which is based on the European standards. 相似文献
92.
Kenichiro Sato Masahiro Kameda Takao Yasuhara Takashi Agari Tanefumi Baba Feifei Wang Aiko Shinko Takaaki Wakamori Atsuhiko Toyoshima Hayato Takeuchi Tatsuya Sasaki Susumu Sasada Akihiko Kondo Cesario V. Borlongan Mitsunori Matsumae Isao Date 《International journal of molecular sciences》2013,14(11):21513-21524
The number of diabetes mellitus (DM) patients is increasing, and stroke is deeply associated with DM. Recently, neuroprotective effects of glucagon-like peptide-1 (GLP-1) are reported. In this study, we explored whether liraglutide, a GLP-1 analogue exerts therapeutic effects on a rat stroke model. Wistar rats received occlusion of the middle cerebral artery for 90 min. At one hour after reperfusion, liraglutide or saline was administered intraperitoneally. Modified Bederson’s test was performed at 1 and 24 h and, subsequently, rats were euthanized for histological investigation. Peripheral blood was obtained for measurement of blood glucose level and evaluation of oxidative stress. Brain tissues were collected to evaluate the level of vascular endothelial growth factor (VEGF). The behavioral scores of liraglutide-treated rats were significantly better than those of control rats. Infarct volumes of liraglutide-treated rats at were reduced, compared with those of control rats. The level of derivatives of reactive oxygen metabolite was lower in liraglutide-treated rats. VEGF level of liraglutide-treated rats in the cortex, but not in the striatum significantly increased, compared to that of control rats. In conclusion, this is the first study to demonstrate neuroprotective effects of liraglutide on cerebral ischemia through anti-oxidative effects and VEGF upregulation. 相似文献
93.
With bolometer application in mind, we prepared VO2 films on TiO2 (001) substrates by an excimer-laser-assisted metal organic deposition process at 300 °C or less. A metal-to-insulator transition of VO2 is expected to induce high temperature coefficient of electrical resistance (TCR) useful for high-performance infrared sensors, but the practical use of crystalline VO2 films has been prevented due to the accompanied wide hysteresis. In this study, by forming the epitaxial phase only near the substrate interface, the transition of the film was successfully broadened and the hysteresis disappeared. The maximum TCR of the film was more than -10%/°C near room temperature, and the temperature range in which TCR was higher than -4%/°C was very wide (280-320 K). 相似文献
94.
Takaaki Noda Doohwan Lee Hyunyoung Shim Masao Sakuraba Takashi Matsuura Junichi Murota 《Thin solid films》2000,380(1-2):57-60
Doping and electrical characteristics of in-situ heavily B-doped Si1−x−yGexCy (0.22<x<0.6, 0<y<0.02) films epitaxially grown on Si(100) were investigated. The epitaxial growth was carried out at 550°C in a SiH4–GeH4–CH3SiH3–B2H6–H2 gas mixture using an ultraclean hot-wall low-pressure chemical vapor deposition (LPCVD) system. It was found that the deposition rate increased with increasing GeH4 partial pressure, and only at high GeH4 partial pressure did it decrease with increasing B2H6 as well as CH3SiH3 partial pressures. With the B2H6 addition, the Ge and C fractions scarcely changed and the B concentration (CB) increased proportionally. The C fraction increased proportionally with increasing CH3SiH3 partial pressures. These results can be explained by the modified Langmuir-type adsorption and reaction scheme. In B-doped Si1−x−yGexCy with y=0.0054 or below, the carrier concentration was nearly equal to CB up to approximately 2×1020 cm−3 and was saturated at approximately 5×1020 cm−3, regardless of the Ge fraction. The B-doped Si1−x−yGexCy with high Ge and C fractions contained some electrically inactive B even at the lower CB region. Resistivity measurements show that the existence of C in the film enhances alloy scattering. The discrepancy between the observed lattice constant and the calculated value at the higher Ge and C fraction suggests that the B and C atoms exist at the interstitial site more preferentially. 相似文献
95.
Yuta Uenoyama Atsushi Matsuda Kazune Ohashi Koji Ueda Misaki Yokoyama Takuya Kyoutou Kouji Kishi Youichi Takahama Masaaki Nagai Takaaki Ohbayashi Osamu Hotta Hideki Matsuzaki 《International journal of molecular sciences》2022,23(9)
Aberrant glycosylation of IgA1 is involved in the development of IgA nephropathy (IgAN). There are many reports of IgAN markers focusing on the glycoform of IgA1. None have been clinically applied as a routine test. In this study, we established an automated sandwich immunoassay system for detecting aberrant glycosylated IgA1, using Wisteria floribunda agglutinin (WFA) and anti-IgA1 monoclonal antibody. The diagnostic performance as an IgAN marker was evaluated. The usefulness of WFA for immunoassays was investigated by lectin microarray. A reliable standard for quantitative immunoassay measurements was designed by modifying a purified IgA1 substrate. A validation study using multiple serum specimens was performed using the established WFA-antibody sandwich automated immunoassay. Lectin microarray results showed that WFA specifically recognized N-glycans of agglutinated IgA1 in IgAN patients. The constructed IgA1 standard exhibited a wide dynamic range and high reactivity. In the validation study, serum WFA-reactive IgA1 (WFA+-IgA1) differed significantly between healthy control subjects and IgAN patients. The findings indicate that WFA is a suitable lectin that specifically targets abnormal agglutinated IgA1 in serum. We also describe an automated immunoassay system for detecting WFA+-IgA1, focusing on N-glycans. 相似文献
96.
Minami Yamauchi Toshihiro Sato Ayana Otake Masaki Kumondai Yu Sato Masafumi Kikuchi Masamitsu Maekawa Hiroaki Yamaguchi Takaaki Abe Nariyasu Mano 《International journal of molecular sciences》2022,23(15)
Patients with liver diseases not only experience the adverse effects of liver-metabolized drugs, but also the unexpected adverse effects of renally excreted drugs. Bile acids alter the expression of renal drug transporters, however, the direct effects of bile acids on drug transport remain unknown. Renal drug transporter organic anion-transporting polypeptide 4C1 (OATP4C1) was reported to be inhibited by chenodeoxycholic acid. Therefore, we predicted that the inhibition of OATP4C1-mediated transport by bile acids might be a potential mechanism for the altered pharmacokinetics of renally excreted drugs. We screened 45 types of bile acids and calculated the IC50, Ki values, and bile acid–drug interaction (BDI) indices of bile acids whose inhibitory effect on OATP4C1 was >50%. From the screening results, lithocholic acid (LCA), glycine-conjugated lithocholic acid (GLCA), and taurine-conjugated lithocholic acid (TLCA) were newly identified as inhibitors of OATP4C1. Since the BDI index of LCA was 0.278, LCA is likely to inhibit OATP4C1-mediated transport in clinical settings. Our findings suggest that dose adjustment of renally excreted drugs may be required in patients with renal failure as well as in patients with hepatic failure. We believe that our findings provide essential information for drug development and safe drug treatment in clinics. 相似文献
97.
Yasutoshi Akiyama Yoshika Takenaka Tomoko Kasahara Takaaki Abe Yoshihisa Tomioka Pavel Ivanov 《International journal of molecular sciences》2022,23(21)
Under stress conditions, transfer RNAs (tRNAs) are cleaved by stress-responsive RNases such as angiogenin, generating tRNA-derived RNAs called tiRNAs. As tiRNAs contribute to cytoprotection through inhibition of translation and prevention of apoptosis, the regulation of tiRNA production is critical for cellular stress response. Here, we show that RTCB ligase complex (RTCB-LC), an RNA ligase complex involved in endoplasmic reticulum (ER) stress response and precursor tRNA splicing, negatively regulates stress-induced tiRNA production. Knockdown of RTCB significantly increased stress-induced tiRNA production, suggesting that RTCB-LC negatively regulates tiRNA production. Gel-purified tiRNAs were repaired to full-length tRNAs by RtcB in vitro, suggesting that RTCB-LC can generate full length tRNAs from tiRNAs. As RTCB-LC is inhibited under oxidative stress, we further investigated whether tiRNA production is promoted through the inhibition of RTCB-LC under oxidative stress. Although hydrogen peroxide (H2O2) itself did not induce tiRNA production, it rapidly boosted tiRNA production under the condition where stress-responsive RNases are activated. We propose a model of stress-induced tiRNA production consisting of two factors, a trigger and booster. This RTCB-LC-mediated boosting mechanism may contribute to the effective stress response in the cell. 相似文献
98.
Tatsunari Ohkubo Takaaki Shiina Kayoko Kawaguchi Daisuke Sasaki Rena Inamasu Yue Yang Zhuoqi Li Keizaburo Taninaka Masaki Sakaguchi Shoko Fujimura Hiroshi Sekiguchi Masahiro Kuramochi Tatsuya Arai Sakae Tsuda Yuji C. Sasaki Kazuhiro Mio 《International journal of molecular sciences》2022,23(23)
Membrane proteins play important roles in biological functions, with accompanying allosteric structure changes. Understanding intramolecular dynamics helps elucidate catalytic mechanisms and develop new drugs. In contrast to the various technologies for structural analysis, methods for analyzing intramolecular dynamics are limited. Single-molecule measurements using optical microscopy have been widely used for kinetic analysis. Recently, improvements in detectors and image analysis technology have made it possible to use single-molecule determination methods using X-rays and electron beams, such as diffracted X-ray tracking (DXT), X-ray free electron laser (XFEL) imaging, and cryo-electron microscopy (cryo-EM). High-speed atomic force microscopy (HS-AFM) is a scanning probe microscope that can capture the structural dynamics of biomolecules in real time at the single-molecule level. Time-resolved techniques also facilitate an understanding of real-time intramolecular processes during chemical reactions. In this review, recent advances in membrane protein dynamics visualization techniques were presented. 相似文献
99.
Xintong Wu Satoi Nagasawa Kasumi Muto Maiko Ueda Chitose Suzuki Takaaki Abe Atsushi Higashitani 《International journal of molecular sciences》2022,23(17)
Mitochonic Acid 5 (MA-5) enhances mitochondrial ATP production, restores fibroblasts from mitochondrial disease patients and extends the lifespan of the disease model “Mitomouse”. Additionally, MA-5 interacts with mitofilin and modulates the mitochondrial inner membrane organizing system (MINOS) in mammalian cultured cells. Here, we used the nematode Caenorhabditis elegans to investigate whether MA-5 improves the Duchenne muscular dystrophy (DMD) model. Firstly, we confirmed the efficient penetration of MA-5 in the mitochondria of C. elegans. MA-5 also alleviated symptoms such as movement decline, muscular tone, mitochondrial fragmentation and Ca2+ accumulation of the DMD model. To assess the effect of MA-5 on mitochondria perturbation, we employed a low concentration of rotenone with or without MA-5. MA-5 significantly suppressed rotenone-induced mitochondria reactive oxygen species (ROS) increase, mitochondrial network fragmentation and nuclear destruction in body wall muscles as well as endogenous ATP levels decline. In addition, MA-5 suppressed rotenone-induced degeneration of dopaminergic cephalic (CEP) neurons seen in the Parkinson’s disease (PD) model. Furthermore, the application of MA-5 reduced mitochondrial swelling due to the immt-1 null mutation. These results indicate that MA-5 has broad mitochondrial homing and MINOS stabilizing activity in metazoans and may be a therapeutic agent for these by ameliorating mitochondrial dysfunction in DMD and PD. 相似文献
100.
Hirofumi Chiba Yukio Suzuki Yoshiaki Yasuda Mitsuyasu Kumagai Takaaki Koyama Shuji Tanaka 《Electrical Engineering in Japan》2021,214(1):62-68
This paper reports a deep‐ultraviolet LED (deep‐UV‐LED) package based on silicon MEMS process technology (Si‐PKG). The package consists of a cavity formed by silicon crystalline anisotropic etching, through‐silicon vias (TSVs) filled with electroplated Cu, bonding metals made of electroplated Ni/AuSn and a quartz lid for hermetic sealing. A deep‐UV LED die is directly mounted in the Si‐PKG by AuSn eutectic bonding without a submount. It has advantages in terms of size, heat dissipation, light utilization efficiency, productivity and cost over conventional AlN ceramic packages. We confirmed a light output of 30 mW and effective reflection on Si (111) cavity slopes in the Si‐PKG. Based on simulation, further improvement of the optical output is expected by optimizing DUV‐LED die mount condition. 相似文献