Pseudomonas fluorescens KKL101, a benzoic acid degrader in a mixed culture that degrades biphenyl and polychlorinated biphenyls

A mixed culture we had isolated, which degrades biphenyl/polychlorinated biphenyls, is composed of two strains, Pseudomonas fluorescens KKL101 and Pseudomonas sp. strain KKS102. KKS102 produces benzoic acid as a dead-end metabolite in the degradation of biphenyl. In this study we showed that KKL101 grew on benzoic acid as a sole source of carbon. This indicated a role of KKS102 in the growth of KKL101 and KKL101 for the growth of KKS102 in the mixed culture.     

An Algorithm for Causal Modeling in Epidemiological Studies

Behaviormetrika - By extending the path analytic method, a new algorithm for causal modeling in epidemiological etiologies is developed, which is based mainly on simple correlation coefficients....     

Flicker noise in MOSFETs made by the DMOS process

Signetics SD 200 MOSFETs show a 1/f^α spectrum with $\text{α ? 1.0}$ at low frequencies and $\text{α ? 0.6}$ at high frequencies, with a turnover at about 50 kHz. The noise resistances in these two branches seem to be related but show a different current dependence. These features may be associated with the “diffused” MOS (DMOS) process by which they are manufactured.     

Effect of gate impurity concentration on inversion-layer mobility in MOSFETs with ultrathin gate oxide layer

In this paper, the influence of poly-Si-gate impurity concentration, N/sub poly/, on inversion-layer electron mobility is experimentally investigated in MOSFETs with ultrathin gate oxide layer. The split capacitance-voltage C-V method is modified to directly measure an effective mobility, paying attention to both 1) accurate current-voltage I-V and capacitance-voltage (C-V) measurements with high gate leakage current and 2) correct surface carrier density, N/sub s/, estimation at a finite drain bias. It is demonstrated that the mobility in ultrathin gate oxides becomes low significantly for highly doped gate, strongly suggesting the contribution of remote Coulomb scattering due to the gate impurities, which is quantitatively discriminated from that of Coulomb scattering due to substrate impurities and interface states. It is also found that the mobility lowering becomes significant rapidly at T/sub ox/ of 1.5 nm or less. The mobility-lowering component is weakly dependent on N/sub s/, irrespective of N/sub poly/, which cannot be fully explained by the existing theoretical models of remote impurity scattering.     

Effect on colon cancer cells of human interferon-beta gene entrapped in cationic multilamellar liposomes

When cultured cells of human colon cancer cell line SW480 were transfected with human interferon-beta (hIFN-beta) gene by means of cationic multilamellar liposomes, the endogenously produced hIFN-beta exhibited a remarkable anti-proliferative effect on the cells, which was more effective than that of exogenously added hIFN-beta. This effect lasted for several days, and was blocked completely by the addition of sufficient amounts of anti-hIFN-beta antibody. From experiments using a transwell plate and an infusion pump, we found that endogenously produced hIFN-beta acted effectively on the cells around the transfectants and that the growth-inhibitory effect was totally retained upon continuous dilution of the medium. These data indicate that hIFN-beta expressed endogenously by transfer of its gene acted on these cancer cells mainly in a paracrine manner. Although the transfection with hIFN-gamma gene also revealed a definite growth-inhibitory effect on the same tumor cells, the extent was less than that of hIFN-beta gene.     

Synthesis of 3,5-bis(m- or m,m′-di-substituted-p-aminophenyl)-n-amino-1,2,4-triazole and its application to benzidine yellow type pigment

3,5-Bis(m- or m,m′-di-substituted-p-aminophenyl)-N-amino-1,2,4-triazoles (4) were synthesized by the oxidative dimerization of the hydrazine adduct of m- or m,m′-di-substituted-p-aminobenzonitriles (3) followed by acid-catalysed rearrangement. They were used to prepare Benzidine Yellow type pigments (8), the thermal stability of which was definitely superior to that of Benzidine Yellow (7) while a slight blue shift of absorption maximum was observed.     

Efficient generation of picosecond coherent tunable radiation between 190 and 212 nm by sum-frequency mixing from Raman and optical parametric radiations

An efficient sum-frequency mixing of optical parametric radiation and stimulated Raman radiation has been used to generate picosecond continuously tunable coherent radiation ranging from 190 to 212 nm with energies of20-40 muJ. An ordinary KDP crystal was phase-matched by using a combination of long and short wavelength incident waves.     

Amination of 5,8-dihydroxy-1,4-naphthoquinone

The reaction of naphthazarin (1) with butylamine selectively gave 2-butylaminonaphthazarin (2a) in 70% yield at low temperature, but it gave complex mixtures of aminated products in very low yields at higher temperature. The similar reactions of 1 with ethylenediamine afforded the ring-closure product, 7-10-dihydroxy-2,3,4-trihydrobenzo]f]quinoxalin-6-one (9a) in 46% yield. It was proposed that the reaction was initiated by the direct 2-amination of 1 followed by the intramolecular nucleophilic substitution of the 2-amino group on the 2-alkylamino substituent, and then followed by oxidation to give the ring-closure product 9a. The role of atmospheric oxygen as an oxidant, and the redox system of 1 in the reaction of 1 with amines, are discussed.     

Coexisting undifferentiated thymic carcinoma and thymic carcinoid tumor

Thymic carcinoma is an unusual neoplasm, and the undifferentiated type is rare. Thymic carcinoid is also rare. This report describes a patient with coexisting undifferentiated thymic carcinoma and a carcinoid tumor. Both lesions were completely excised. The carcinoid cells showed argyrophilic granules by Grimelius' method and immunoreactivity against neuron-specific enolase, whereas the undifferentiated carcinoma cells were negative for argyrophilic stain and immunostaining against neuron-specific enolase.