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排序方式: 共有1730条查询结果,搜索用时 15 毫秒
71.
Takayuki Ueki Akifumi Yoshihara Yuji Teramura 《Science and Technology of Advanced Materials》2016,17(1):807-815
Since circulating tumor cells (CTCs) are tumor cells which are found in the blood of cancer patients, CTCs are potential tumor markers, so a rapid isolation of CTCs is desirable for clinical applications. In this paper, a three-dimensional polystyrene (PS) microfiber fabric with vacuum aspiration system was developed for capturing CTCs within a short time. Various microfiber fabrics with different diameters were prepared by the electrospinning method and optimized for contact frequency with cells. Vacuum aspiration utilizing these microfiber fabrics could filter all cells within seconds without mechanical damage. The microfiber fabric with immobilized anti-EpCAM antibodies was able to specifically capture MCF-7 cells that express EpCAM on their surfaces. The specificity of the system was confirmed by monitoring the ability to isolate MCF-7 cells from a mixture containing CCRF-CEM cells that do not express EpCAM. Furthermore, the selective capture ability of the microfiber was retained even when the microfiber was exposed to the whole blood of pigs spiked with MCF-7 cells. The specific cell capture ratio of the vacuum aspiration system utilizing microfiber fabric could be improved by increasing the thickness of the microfiber fabric through electrospinning time. 相似文献
72.
Kimihiro Yamanaka Takafumi Ooyoshi Takayuki Nejime 《Journal of Materials Science: Materials in Electronics》2010,21(1):53-57
Measuring the real temperature of a flip chip solder joint during an electromigration test has been a problem because of its
small size and the local Joule heating due to a large applied current. A unique method that employs the electrical resistance
change in the junction line between two solder joints was introduced to determine the temperature of the solder joint. The
change in resistance was converted into temperature using a thermal coefficient of resistance of the junction line. The method
accurately measured temperatures of the solder joint within 2 K until the solder joint resistance change ratio reached 100%
due to growth of an electromigration void. 相似文献
73.
Takayuki Wada Author Vitae Author Vitae 《Automatica》2010,46(8):1339-1345
Efficient randomized algorithms are developed for solving robust feasibility problems with multiple parameter-dependent convex constraints. Two complementary strategies are presented, both of which exploit the multiplicity to achieve fast convergence. One is the stochastic ellipsoid method with multiple updates. In each iteration of this algorithm, an ellipsoid which describes a candidate of the solution set is updated many times via the multiple constraints with one random sample, while at most one update is allowed in the original method. The other is the stochastic ellipsoid method with multiple cuts. Here, a new update rule is presented to construct a smaller ellipsoid directly via multiple subgradients given by the constraints. A quantitative analysis of the volume of the ellipsoid is also provided, which guarantees the advantage of the proposed algorithm over the original one. The above features lead to a reduction of the total number of random samples necessary for convergence, which is extensively demonstrated through numerical examples. 相似文献
74.
Takahiro Kawashima Tsuyoshi Kimura Takayuki Shibata Akio Kishida Takashi Mineta Eiji Makino 《Microelectronic Engineering》2010,87(5-8):704-707
In order to realize cellular network analysis on a chip-based system, our group has been developing a patterned cell culture microdevice with pillars in an array for tapping cells into space surrounded by the pillars. The pillar structures has advantages to trap both adhesive and non-adhesive cells and to precisely control positions of cells and distances between cells for understanding effects of various cell patterns on functions of a cellular network such as cell proliferation, differentiation, and network formation. In this paper, HeLa cell cultivation with the patterned cell culture microdevice having a pillar array fabricated by dry film of thick negative photoresist SU-8 on a glass substrate was executed as a feasibility study on a cellular network analysis. The results revealed that the device performance was found to be enough to culture HeLa cells for more than 48 h. In addition, relative extensibility of blocks of multiple cells compared with single cells tapped on the device was observed. Thus, the patterned cell culture microdevice proposed here could be applicable to analysis of cellular functions. 相似文献
75.
Mitsutoshi Okada Tohru Hisamatsu Takayuki Kitamura 《Journal of Thermal Spray Technology》2009,18(1):90-95
A superalloy with a thermal barrier coating (TBC) simulating a gas turbine blade is exposed to a high-temperature environment
to develop a method for predicting the local temperature and Al content in a bond coat (BC). The Al content decreases with
an increase in the test time due to the Al transport induced by the oxidation of the BC and the interdiffusion between the
BC and the substrate. This brings about Al-decreased layer (ADL) at the boundary between the BC and the top coat. The thickness
of the ADL increases in proportion to the square root of the test time, and the temperature dependence of the growth rate
shows an Arrhenius-type behavior. Based on this relation, the local temperature of an in-service blade can be estimated by
measuring the ADL thickness when the operation time is known. The Al content decreases in proportion to the ADL thickness.
The prediction method of the Al content based on the relation is also presented. 相似文献
76.
77.
Rare-earth (RE: Lu, Gd, Nd, 0.10 mol%)-doped alumina nanopowders were prepared by a new sol-gel route using polyhydroxoaluminum (PHA) and RECl3 solutions under α-alumina (∼ 75 nm) seeding. Among the rare-earth dopants studied, Lu yields the most suitable nanopowders for low-temperature densification. The 0.10 mol% Lu-doped nanopowders, which were obtained at a calcination temperature of 900 °C under 5 mass% α-alumina seeding, consisted of ∼ 80-nm α-alumina particles and γ-alumina nanoparticles. Using these Lu-doped alumina nanopowders, fully densified alumina ceramics with a uniform microstructure composed of fine grains with an average size of 0.61 μm could be obtained at 1400 °C by pressureless sintering. Clearly, the Lu-doped nanopowders obtained here represent a viable option for fabricating dense, finer-grained alumina ceramics because an undoped sample with 5 mass% seeds gave a microstructure with an average grain size of 1.78 μm at 1400 °C. 相似文献
78.
To identify the differences in the knowledge production between disciplines, we analyzed the relation between the average
paper length and impact factor of 100 journals from 5 disciplines. We found negative correlation between the average length
and the impact factor in the natural sciences, but not in the social sciences. We also analyzed the structures of paper and
the citation patterns. These analyses are expanded to the comparison between Mode 1 and Mode 2. All results showed the natural
sciences articles could emphasize the differences from previous studies and be diffused effectively by the short standardized
style of paper.
This research is partly funded by International Program in Special Coordinating Funds for Promoting S&T from Science and Technology
Agency of Japan. 相似文献
79.
Satoru Hiroshige Hirotoshi Asano Masafumi Uchida Hideto Ide 《Artificial Life and Robotics》2009,14(1):67-70
Recently, there has been much interest in the study of the formation of groups of agents that cause interactions between agents and invent new functions. We gave some agents an action rule based on the interactions of human feelings by using a circumplex model. It had been decided that the parameters of feelings in this model should have only two axes. In this report, eight basic action dimensions and pure feelings on four corresponding axes were given to agents as a model of feelings and actions based on the multiple factor analysis theory of R. Plutchik, and the behavioral characteristics of the group of agents were examined. 相似文献
80.
Increase of stress intensity near interface edge of elastic-creep Bi-material under a sustained load
The transition from small-scale creep to large-scale creep ahead of a crack tip or an interface edge with strong elastic stress singularity at the loading instant causes stress relaxation and the decrease of stress intensity in general. However, this study shows that the stress near the interface edge of bi-material with no or weak elastic stress singularity increases after the loading instant and brings about the stress concentration during the transition. In addition, the creep strain distribution of this bi-material after the loading instant is different from that occurred in the transition of an interface edge with strong elastic stress singularity or a crack tip (notch root). The criterion for the increase or decrease of stress intensity near the interface edge proved by the finite element method is proposed in this study. The stress intensity near the interface edge increases when the elastic stress singularity is lower than the creep stress singularity (λel < λcr) and vice versa. 相似文献