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941.
Matsuya T Tashiro S Hoshino N Shibata N Nagasaki Y Kataoka K 《Analytical chemistry》2003,75(22):6124-6132
To increase the sensitivity and to depress the nonspecific binding in biochemical assays, a new core-shell-type fluorescent nanosphere (106.7 nm) covalently conjugated with antibody was prepared. The core-shell-type nanosphere was constructed by dispersion radical polymerization of styrene in the presence of heterotelechelic poly(ethylene glycol) (PEG) macromonomer, which has a polymerizable vinylbenzyl group at one end and a primary amino group at the other chain end and used as well as a surfactant. The resulting nanosphere had PEG tethered chains on the surface, which possesses a primary amino group at the distal end of the PEG chain (NH(2) nanosphere). The fluorescent NH(2) nanosphere was constructed by incorporating fluorescent europium chelates with beta-diketonate ligands in the core of the NH(2) nanosphere by means of a physical entrapment method. The primary amino groups on the fluorescent NH(2) nanosphere were then converted to maleimide groups using a hetero cross-linker. The resulting nanosphere had maleimide groups on the surface (maleimide nanosphere), onto which proteins having SH group in the molecule could be covalently conjugated quantitatively without any denaturation of the proteins under the milder reaction condition. The applicability of the fluorescent nanosphere was tested in a model sandwich immunoassay for alpha-fetoprotein (AFP) determination. Anti-human AFP Fab' fragment was covalently conjugated onto the maleimide nanosphere (Fab' nanosphere), and it was used for the solid-phase time-resolved fluorometric immunoassay of AFP. The detection limit (mean + 2 SD) was 0.040 pg/mL or 57.1 zmol (57.1 x 10(-)(21) mol, M(w,AFP) = 70000) for AFP. The imprecision (concentration CV) over the whole assay range was 1.1% (100 pg/mL) - 17.1% (0.1 pg/mL), even though with this conjugation of antibody to the nanosphere, the nonspecific binding was practically negligible (0.0008%) and even when approximately 1.9 x 10(9) particles of the Fab' nanosphere were applied to the microtitration well. 相似文献
942.
Kuhn A Fox MD French PW Hettrick S Hand DP Shi YW Matsuura Y Miyagi M Watkins KG Ireland CL Jones JD 《Applied optics》2003,42(25):5101-5106
A major advantage of fiber-optic beam delivery in laser materials processing is the ability to guide the laser power to the location where it is needed, leaving the laser itself remote and protected from the process. This is of special importance if the processing is to be performed in a hazardous environment. Particular problems are faced by the nuclear industry where weld repair and surface treatment work are required inside radioactive installations. By use of fiber beam delivery, only part of the delivery system and effector optics become contaminated, but the expensive laser system does not. However, in many cases the region where repair is required is not only radioactive but has only limited physical access, e.g., inside tubes or into corners, which prevents use of standard effector optics. We present a new design to deal with such constraints of a 2-mm outer diameter employing a hollow waveguide and gas shielding. This design is optically characterized and its performance assessed in welding and surface treatment applications. The potential of this compact effector optics in limited physical access situations is clearly demonstrated. 相似文献
943.
A beam homogenizer for a hollow-fiber-based, UV laser delivery system is proposed. A rectangular glass waveguide with an inner aluminum coating that has a 1-mm square cross section is attached at the output end of the circular-core hollow fiber with a 1-mm inner diameter. The rectangular waveguide generates a number of higher-order modes and results in a uniform top-hat profile. The configuration of the waveguide is designed by a ray-tracing technique so that both the low transmission loss and the high uniformity in the output beam are obtained. The fabricated waveguide shows a low loss of 0.4 dB, and the intensity variation coefficient is 7%. The output beam from the rectangular waveguide is expanded by a lens to the size larger than 10-mm square. It is also shown that the profile does not change with the bending condition. 相似文献
944.
Pulse voltammetric and ac impedance spectroscopic studies on lithium ion transfer at an electrolyte/Li4/3Ti5/3O4 electrode interface 总被引:1,自引:0,他引:1
Pulse voltammetry and ac impedance spectroscopy were used to study the lithium ion kinetics at a lithium ion insertion electrode consisting of Li4/3Ti5/3O4 thin films in an organic electrolyte. In the cyclic voltammogram, two redox peaks appeared at around 1.56 V vs Li/Li+ due to the insertion and extraction of lithium ion at the electrode. Differential pulse voltammetry gave a large reduction current at approximately 1.56 V during a cathodic scan due to lithium ion insertion into the electrode. From the peak current and potential, the charge-transfer resistance was evaluated by quantitative analysis using approximate equations for irreversible reactions. In the Nyquist plot, one semicircle was observed at 1.56 V, which was assigned to the charge-transfer resistance due to lithium ion transfer at the electrode/electrolyte interface. The value of the charge-transfer resistance at 1.56 V was almost identical to that evaluated by differential pulse voltammetry with an identical characteristic relaxation time. This result shows that both dc differential pulse voltammetry and ac impedance spectroscopy are useful for elucidating the phase transfer kinetics of lithium ion at insertion electrodes. 相似文献
945.
Sawada H Tomita T Naruse M Honda T Hambridge P Hartel P Haider M Hetherington C Doole R Kirkland A Hutchison J Titchmarsh J Cockayne D 《Journal of electron microscopy》2005,54(2):119-121
We have successfully developed a spherical aberration (Cs)-corrected electron microscope for probe- and image-forming systems using hexapole correctors. The performance of the microscope has been evaluated experimentally. The point resolution attained using the image-forming Cs-corrector is better than 0.12 nm. For scanning transmission electron microscopy, the Ronchigram flat area was >40 mrad in half-angle using the probe-forming Cs-corrector. 相似文献
946.
Mano T Kuroda T Sanguinetti S Ochiai T Tateno T Kim J Noda T Kawabe M Sakoda K Kido G Koguchi N 《Nano letters》2005,5(3):425-428
We demonstrate the self-assembled formation of concentric quantum double rings with high uniformity and excellent rotational symmetry using the droplet epitaxy technique. Varying the growth process conditions can control each ring's size. Photoluminescence spectra emitted from an individual quantum ring complex show peculiar quantized levels that are specified by the carriers' orbital trajectories. 相似文献
947.
Removal of B from Si by solidification refining with Si-Al melts 总被引:2,自引:0,他引:2
To discuss the removal of B by solidification refining of Si with a Si-Al melt, the segregation of B between solid Si and
the Si-Al melt was investigated by use of the temperature-gradient-zone melting (TGZM) method. The segregation ratio of B
at its infinite dilution was determined to be 0.49 (1473 K), 0.32 (1373 K), and 0.22 (1273 K), respectively. With the obtained
segregation ratio, the activity coefficient of B in solid Si at its infinite dilution relative to pure solid B was determined
by the following equation:
Calculated results of directional solidification of the Si-Al alloy revealed the removal fraction of B to be as much as 90
pct. The effective removal of B by a solidification refining process with a Si-Al melt is clarified. 相似文献
948.
Effect of sulfur sources on specific desulfurization activity of Rhodococcus erythropolis KA2-5-1 in exponential fed-batch culture 总被引:1,自引:0,他引:1
Konishi M Kishimoto M Omasa T Katakura Y Shioya S Ohtake H 《Journal of Bioscience and Bioengineering》2005,99(3):259-263
The effects of sulfur sources on the desulfurization activity of Rhodococcus erythropolis KA2-5-1 were investigated by using an exponential fed-batch culture technique. The feed rate of a sulfur source was controlled independently of the feed rate of ethanol, which was used as a carbon and energy source. Among the sulfur sources examined were dibenzothiophene (DBT), ammonium sulfate, L-cysteine, L-methionine, and 2-amino-ethanesulfonic acid. When the fed-medium contained DBT as the sole sulfur source, KA2-5-1 cells showed a maximum desulfurization activity of approximately 130 mmol 2-HBP kg-cell(-1) h(-1). Similar levels of enzyme activity were also achieved with inexpensive ammonium sulfate by using the exponential fed-batch culture technique. In addition, higher levels of desulfurization activity were achieved by increasing the dosage of the DBT desulfurization (dsz) operon and dszD gene in R. erythropolis KA2-5-1. The recombinant strain showed a maximum desulfurization activity of approximately 250 mmol 2-HBP kg-cell(-1) h(-1) in the exponential fed-batch cultures. 相似文献
949.
Junichiro?OtomoEmail author Takanori?Tamaki Satoru?Nishida Shuqiang?Wang Masaru?Ogura Takeshi?Kobayashi Ching-ju?Wen Hidetoshi?Nagamoto Hiroshi?Takahashi 《Journal of Applied Electrochemistry》2005,35(9):865-870
The proton conduction and superionic phase transition of cesium dihydrogen phosphate, CsH2PO4 (CDP), were investigated under various humid conditions to evaluate the applicability of a CsH2PO4 solid electrolyte to an intermediate temperature fuel cell operating between 230 °C and 300 °C. The phase stability, superionic phase transition, and reversibility of dehydration of CsH2PO4 were evaluated under different ambient water vapor concentrations, from 0 to 90 mol%, through the measurements of conductivity. The dependence of conductivity on the water vapor concentration and the demonstrated reversibility of dehydration clearly showed the range in which CsH2PO4 is applicable to the intermediate temperature fuel cell. Additionally, we evaluated the protonic transport number of CsH2PO4, which was almost unity, and demonstrated fuel cell operation at 250 °C using a single cell fabricated with the CsH2PO4 electrolyte. 相似文献
950.
Takeshi?TomaEmail author Atsushi?Ishida Paulus?Matius 《Nutrient Cycling in Agroecosystems》2005,71(1):63-72
Between 1988 and 2000, changes in the above-ground biomass (AGB) of trees in an East Kalimantan lowland forest, damaged by fires in 1982–83 and 1998, were estimated using allometric functions and an annual inventory of stem diameter. The original vegetation of the study site was lowland dipterocarp forest which has since been affected by selective logging and wild fires. The 1982–83 fire killed large trees of primary species and the opened sites became dominated by a few pioneer species. Between 1988 and 1997, a few pioneer tree species, namely Macaranga spp., dominated a heavily disturbed stand (HDS). Primary tree species that survived the 1982–83 fire dominated a lightly disturbed stand (LDS). A moderately disturbed stand (MDS) contained vegetation intermediate between the HDS and the LDS. In 1997, there were 553, 499 and 356 trees ha-1 in the HDS, MDS and LDS, respectively. Macaranga trees accounted for 70, 40 and 11 of the total number of trees in the HDS, MDS, and LDS, respectively. In 1997, the AGB of trees in the HDS, MDS, and LDS was 117, 280, and 315 Mg ha-1, respectively. The proportion of biomass accounted for by Macaranga trees for the HDS, MDS, and LDS was 34, 8 and 1, respectively. The pioneer trees did not compensate for the loss of aboveground biomass resulting from the death of large primary trees. The fire in 1998 again decreased AGB of the stands. In 2000, the AGB of trees in the HDS, MDS, and LDS was 27, 106, and 219 Mg ha-1, respectively. The sites opened up by the 1998 fire were covered with the pioneer seedlings and seemingly dominated by the pioneer trees with a larger number of stems per ha, but lower biomass as compared to the original forest (> 400 Mg ha-1). 相似文献