Impact of virus aggregation on inactivation by peracetic acid and implications for other disinfectants

Viruses in wastewater and natural environments are often present as aggregates. The disinfectant dose required for their inactivation, however, is typically determined with dispersed viruses. This study investigates how aggregation affects virus inactivation by chemical disinfectants. Bacteriophage MS2 was aggregated by lowering the solution pH, and aggregates were inactivated by peracetic acid (PAA). Aggregates were redispersed before enumeration to obtain the residual number of individual infectious viruses. In contrast to enumerating whole aggregates, this approach allowed an assessment of disinfection efficiency which remains applicable even if the aggregates disperse in post-treatment environments. Inactivation kinetics were determined as a function of aggregate size (dispersed, 0.55 and 0.90 μm radius) and PAA concentration (5-103 mg/L). Aggregation reduced the apparent inactivation rate constants 2-6 fold. The larger the aggregate and the higher the PAA concentration, the more pronounced the inhibitory effect of aggregation on disinfection. A reaction-diffusion based model was developed to interpret the experimental results, and to predict inactivation rates for additional aggregate sizes and disinfectants. The model showed that the inhibitory effect of aggregation arises from consumption of the disinfectant within the aggregate, but that diffusion of the disinfectant into the aggregates is not a rate-limiting factor. Aggregation therefore has a large inhibitory effect if highly reactive disinfectants are used, whereas inactivation by mild disinfectants is less affected. Our results suggest that mild disinfectants should be used for the treatment of water containing viral aggregates.     

Effective time step restrictions for explicit MPM simulation

Time steps for explicit MPM simulation in computer graphics are often selected by trial and error due to the challenges in automatically selecting stable time step sizes. Our time integration scheme uses time step restrictions that take into account forces, collisions, and even grid-to-particle transfers calculated near the end of the time step. We propose a novel set of time step restrictions that allow a time step to be selected that is stable, efficient to compute, and not too far from optimal. We derive the general solution for the sound speed in nonlinear isotropic hyperelastic materials, which we use to enforce the classical CFL time step restriction. We identify a single-particle instability in explicit MPM integration and propose a corresponding time step restriction in the fluid case. We also propose a reflection-based boundary condition for domain walls that supports separation and accurate Coulomb friction while preventing particles from penetrating the domain walls.     

Co-Doped MoSe2 Nanoflowers as Efficient Catalysts for Electrochemical Hydrogen Evolution Reaction (HER) in Acidic and Alkaline Media

Transition metal dichalcogenides (TMDCs) based materials are considered as highly active alternatives to the precious Pt-based catalysts for the hydrogen evolution reaction (HER). In particular, MoSe₂ emerges as a promising catalyst due to its abundance and electrochemical stability, but further modifications are still required to enhance its performance, specifically in alkaline conditions. Here, we developed a method to obtain MoSe₂ with two cobalt doping patterns: homogeneously doped and edge doped nanoflowers, with abundant edge sites and extended surface area. The results show that low concentration of doping enhances the catalytic activity toward HER. Incorporation of cobalt as a substituent dopant within the layered structure of MoSe₂ appears to have two major contributions: it changes the chemical environment providing more active sites with favored hydrogen adsorption properties, and improves the charge transfer resistance and thus facilitates the HER kinetics. Moreover, the homogeneous and edge-doped nanoflowers show different pH-dependence of HER activity. Edge-doped samples exhibit significantly improved performance in acidic medium, while the overpotential increases in alkaline environment upon doping. A mechanistic explanation of the observed effect is proposed. This work opens up an additional path for improving the catalytic activity of TMDCs in acidic or alkaline medium using a simple and facile method with only small quantities of dopants.     

Frequency drives lexical access in reading but not in speaking: The frequency-lag hypothesis.

To contrast mechanisms of lexical access in production versus comprehension we compared the effects of word frequency (high, low), context (none, low constraint, high constraint), and level of English proficiency (monolingual, Spanish–English bilingual, Dutch–English bilingual) on picture naming, lexical decision, and eye fixation times. Semantic constraint effects were larger in production than in reading. Frequency effects were larger in production than in reading without constraining context but larger in reading than in production with constraining context. Bilingual disadvantages were modulated by frequency in production but not in eye fixation times, were not smaller in low-constraint contexts, and were reduced by high-constraint contexts only in production and only at the lowest level of English proficiency. These results challenge existing accounts of bilingual disadvantages and reveal fundamentally different processes during lexical access across modalities, entailing a primarily semantically driven search in production but a frequency-driven search in comprehension. The apparently more interactive process in production than comprehension could simply reflect a greater number of frequency-sensitive processing stages in production. (PsycINFO Database Record (c) 2011 APA, all rights reserved)     

Phase and polarization control as a route to plasmonic nanodevices

We extend the concepts of phase, polarization, and feedback control of matter to develop a general approach for guiding light in the nanoscale via nanoparticle arrays. The phase and polarization of the excitation source are first introduced as tools for control over the pathway of light at array intersections. Genetic algorithms are next applied as a systematic design tool, wherein both the excitation field parameters and the structural parameters of the nanoparticle array are optimized to make devices with desired functionality. Implications to research fields such as single molecule spectroscopy, spatially confined chemistry, optical logic, and nanoscale sensing are envisioned.     

640 Gb/s Timing Jitter-Tolerant Data Processing Using a Long-Period Fiber-Grating-Based Flat-Top Pulse Shaper

We report on the use of a novel all-fiber flat-top pulse shaping technique for improving performance and timing jitter tolerance of a switch made for 640-10 Gb/s signal demultiplexing. The jitter tolerance is increased to almost 30% of the one-bit time window, and an increase of the receiver sensitivity by 13 dB compared to a nonflat-top pulse is reported.     

The role of cue salience in the development of time concepts: Duration comparisons in young children.

It has been claimed that in the course of developing time concepts, young children go through a stage in which they conceptualize duration as being dependent on endings of events rather than on beginnings and endings. This conceptual deficit was proposed to account for children's greater success in comparing durations that differ in their endings than durations that differ in their beginnings. The present study analyzes the possibility that this phenomenon may be attributed mainly to perceptual salience rather than to conceptual deficit. 360 children from nursery school, kindergarten, and 1st grade were asked to compare the burning times of 2 lights that started and/or ended simultaneously. The role of attention to beginning vs end points in duration judgment was investigated by manipulating beginning- vs end-point salience via systematic variations of sequence of the problems presented. Correct comparisons of duration problems were found to be a function of the relative salience of beginning vs end points. Results support the salience attention model rather than the conceptual deficit account of duration judgments of young children. (17 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Intramuscular Exposure to a Lethal Dose of Ricin Toxin Leads to Endothelial Glycocalyx Shedding and Microvascular Flow Abnormality in Mice and Swine

Ricin toxin isolated from the castor bean (Ricinus communis) is one of the most potent and lethal molecules known. While the pathophysiology and clinical consequences of ricin poisoning by the parenteral route, i.e., intramuscular penetration, have been described recently in various animal models, the preceding mechanism underlying the clinical manifestations of systemic ricin poisoning has not been completely defined. Here, we show that following intramuscular administration, ricin bound preferentially to the vasculature in both mice and swine, leading to coagulopathy and widespread hemorrhages. Increased levels of circulating VEGF and decreased expression of vascular VE-cadherin caused blood vessel impairment, thereby promoting hyperpermeability in various organs. Elevated levels of soluble heparan sulfate, hyaluronic acid and syndecan-1 were measured in blood samples following ricin intoxication, indicating that the vascular glycocalyx of both mice and swine underwent extensive damage. Finally, by using side-stream dark field intravital microscopy imaging, we determined that ricin poisoning leads to microvasculature malfunctioning, as manifested by aberrant blood flow and a significant decrease in the number of diffused microvessels. These findings, which suggest that glycocalyx shedding and microcirculation dysfunction play a major role in the pathology of systemic ricin poisoning, may serve for the formulation of specifically tailored therapies for treating parenteral ricin intoxication.     

Quality of informal care is multidimensional.

Purpose: To demonstrate that assessing quality of informal care involves more than merely determining whether care recipient needs for assistance with activities of daily living (ADLs) are routinely satisfied. We investigated the extent to which potentially harmful behavior (PHB), adequate care, and exemplary care (EC) are empirically distinct dimensions of quality of care. Design: 237 care recipients completed the quality of care measures, and their caregivers completed psychosocial measures of their own depression, life events, cognitive status, and perceptions of pre-illness relationship quality. Results: Although PHB was moderately related to EC, adequate care was not associated with PHB and was only slightly related to EC. Psychosocial variables were not related to adequate care but were differentially associated with PHB and EC, providing additional evidence for the distinction between these measures of quality of care. Conclusions: ADL assistance can be adequate in the presence of PHB and/or the absence of EC. Declines in EC may signal increases in PHB, independent of adequacy of care. These findings produce a brief, portable, and more comprehensive instrument for assessing quality of informal care. (PsycINFO Database Record (c) 2010 APA, all rights reserved)