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91.
Alexander Haseloer Tamara Lützenburg Joss Pepe Strache Dr. Jörg Neudörfl Prof. Dr. Ines Neundorf Prof. Dr. Axel Klein 《Chembiochem : a European journal of chemical biology》2021,22(4):694-704
Three chiral tridentate N^N^S coordinating pyridine-carbaldehyde (S)-N4-(α-methylbenzyl)thiosemicarbazones (HTSCmB) were synthesised along with lysine-modified derivatives. One of them was selected and covalently conjugated to the cell-penetrating peptide sC18 by solid-phase peptide synthesis. The HTSCmB model ligands, the HTSCLp derivatives and the peptide conjugate rapidly and quantitatively form very stable PtII chlorido complexes [Pt(TSC)Cl] when treated with K2PtCl4 in solution. The Pt(CN) derivatives were obtained from one TSCmB model complex and the peptide conjugate complex through Cl−→CN− exchange. Ligands and complexes were characterised by NMR, IR spectroscopy, HR-ESI-MS and single-crystal XRD. Intriguingly, no decrease in cell viability was observed when testing the biological activity of the lysine-tagged HdpyTSCLp, its sC18 conjugate HdpyTSCL-sC18 or the PtCl and Pt(CN) conjugate complexes in three different cell lines. Thus, given the facile and effective preparation of such Pt-TSC-peptide conjugates, these systems might pave the way for future use in late-stage labelling with Pt radionuclides and application in nuclear medicine. 相似文献
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Understanding how spaces in buildings are being used is vital for optimizing space utilization, for improving resource allocation, and for the design of new facilities. We present a multi-year design study that resulted in Ocupado, a set of visual decision-support tools centered around occupancy data for stakeholders in facilities management and planning. Ocupado uses WiFi devices as a proxy for human presence, capturing location-based counts that preserve privacy without trajectories. We contribute data and task abstractions for studying space utilization for combinations of data granularities in both space and time. In addition, we contribute generalizable design choices for visualizing location-based counts relating to indoor environments. We provide evidence of Ocupado's utility through multiple analysis scenarios with real-world data refined through extensive stakeholder feedback, and discussion of its take-up by our industry partner. 相似文献
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Tamara Schenekar Martin Schletterer Laurne A. Lecaudey Steven J. Weiss 《河流研究与利用》2020,36(7):1004-1013
Biodiversity monitoring via environmental DNA, particularly metabarcoding, is evolving into a powerful assessment tool for riverine systems. However, for metabarcoding to be fully integrated into standardized monitoring programmes, some current challenges concerning sampling design, laboratory workflow, and data analysis need to be overcome. Here, we review some of these major challenges and potential solutions. We further illustrate three potential pitfalls, namely the choice of suitable metabarcoding primers, the necessity of complete reference databases, and varying assay sensitivities, by a reappraisal of our‐own recently carried out metabarcoding study in the Volga headwaters. TaqMan qPCRs had detected catfish (Silurus glanis) and European eel (Anguilla anguilla), whereas metabarcoding had not, in the same samples. Furthermore, after extending the genetic reference database by 12 additional species and re‐analysing the metabarcoding data, we additionally detected the Siberian spiny loach (Cobitis sibirica) and Ukrainian brook lamprey (Eudontomyzon mariae) and reassigned the operational taxonomic units previously assigned to Misgurnus fossilis to Cobitis sibirica. In silico analysis of metabarcoding primer efficiencies revealed considerable variability among primer pairs and among target species, which could lead to strong primer bias and potential false‐negatives in metabarcoding studies if not properly compensated for. These results highlight some of the pitfalls of eDNA‐metabarcoding as a means of monitoring fish biodiversity in large rivers, which need to be considered in order to fully unleash the full potential of these approaches for freshwater biodiversity monitoring. 相似文献
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Tommasi Tatiana Mallya Arun Plummer Bryan Lazebnik Svetlana Berg Alexander C. Berg Tamara L. 《International Journal of Computer Vision》2019,127(1):38-60
International Journal of Computer Vision - This paper presents an approach for answering fill-in-the-blank multiple choice questions from the Visual Madlibs dataset. Instead of generic and commonly... 相似文献
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Eran Ivanir Yonatan Shachaf Iris Mironi‐Harpaz Daniella Yeheskely‐Hayon Lena Hazanov Shlomit Harpaz‐Segev Tamara Birman Limor Minai Sonia Melino Dvir Yelin Dror Seliktar 《Advanced functional materials》2020,30(18)
The ability to precisely control cell‐loaded material systems is essential for in vitro testing of novel therapeutics poised to advance to clinic. In this report, unique patterns of cell migration are devised into an in vitro gel‐in‐gel model for the purpose of obtaining cell response data to potentially therapeutic chemical agonists. The model consists of co‐cultures in a cell‐loaded microgel invading an acellular “sorting” gel. Material properties including biophysical and chemical compositions of the sorting gel are carefully controlled to guide a desired cell‐specific behavior, leading to massive tumor cell invasion by amoeboid migration mechanisms. Optical transparency enables straightforward and high‐throughput measurements of outgrowth response in the presence of either chemical and photoradiation therapy. Important dosing and drug sensitivity information are obtained with the gel‐in‐gel model using no more than a light microscope, without further need for arduous genomic or proteomic screening of the tissue samples. 相似文献