Computer reconstruction of luminal cross-sectional shape from multiple cineangiographic views

Knowledge of coronary luminal shape, in addition to diameter information as routinely obtained from a cineangiogram, may be useful in assessing lesions which deviate from circular symmetry. We have developed an image analysis system for automated tracking of luminal edges and measurement of diameter form cine frames digitized by a video camera/digitizer interfaced to a Vax 11/780 computer. Between vessel edges, cinedensitometric profiles across the vessel long axis are used to provide a rotationally invariant measure of relative luminal cross-sectional area. A maximum entropy iterative algorithm is used to reconstruct the lumen cross section from a set of projection data consisting of the cinedensitometric profiles from multiple radiographic views. Nonaxisymmetric model coronary lumena, such as a crescent shape and a double lumen simulating a coronary artery dissection, were filmed under cineradiographic conditions similar to clinical exposures. Radiographic views at 10 degrees increments about the model lumen long axis over 360 degrees were available for analysis. Graphic display of reconstructed model lumena indicate that as few as three to five radiographic views may be useful in reconstructing coronary luminal shape.     

Properties of the n-methyl-2-pyrrolidinone-water system

Densities, excess volumes and heats of mixing, heat capacities, refractive indices and viscosities of various N-methyl-2-pyrrolidinone (NMP)-water mixtures are reported. The excess volumes and heat of mixing are negative and show their greatest deviations from ideality at 0.3 - 04 mole fraction NMP. The viscosity of the system passes through a maximum in the same region of solvent composition. These data indicate strong interaction between NMP and water and suggest the existence of transient polymeric species of the type — (NMP · 2H₂O)n?.     

The functionality-based application confinement model

This paper presents the functionality-based application confinement (FBAC) access control model. FBAC is an application-oriented access control model, intended to restrict processes to the behaviour that is authorised by end users, administrators, and processes, in order to limit the damage that can be caused by malicious code, due to software vulnerabilities or malware. FBAC is unique in its ability to limit applications to finely grained access control rules based on high-level easy-to-understand reusable policy abstractions, its ability to simultaneously enforce application-oriented security goals of administrators, programs, and end users, its ability to perform dynamic activation and deactivation of logically grouped portions of a process’s authority, its approach to process invocation history and intersection-based privilege propagation, its suitability to policy automation techniques, and in the resulting usability benefits. Central to the model are ‘functionalities’, hierarchical and parameterised policy abstractions, which can represent features that applications provide; ‘confinements’, which can model simultaneous enforcement of multiple sets of policies to enforce a diverse range of types of application restrictions; and ‘applications’, which represent the processes to be confined. The paper defines the model in terms of structure (which is described in five components) and function, and serves as a culmination of our work thus far, reviewing the evaluation of the model that has been conducted to date.     

Determination and analysis of the electrical components of a PEF treated equivalent circuit of potato tissue

The permeabilized membrane state of a pulsed electric field (PEF) treated biological sample is invisible to the naked eye, but can be deduced as the permeabilization causes loss of the insulating properties of the membrane, which can be determined by measuring the electrical impedance. This paper reports a spectroscopic analysis of a PEF treated and untreated potato sample to determine the electrical components of the biological tissue equivalent circuit model. The measurements concern flat potato samples which were subjected to different values of factors such as electric field, pulse number, pulse width and frequency. Cole–Cole plots are used to calculate the pore resistance and capacitance. This method of impedance measurement is used to establish the appropriate equivalent circuit model of the potato tissue according to the measured data, and thus explanations about the cell model from the impedance spectra are deduced. The PEF parameters had significant effect on the impedance and structural changes of the biological tissue. The treatment frequency does not have significant effect on the impedance when the electrical field, pulse number and width are kept constant.     

Predicting the response of Hamilton Harbour to the nutrient loading reductions: A modeling analysis of the “ecological unknowns”

We examine the likelihood of delisting the Hamilton Harbour as an Area of Concern, if the nutrient loading reductions proposed by the Remedial Action Plan are actually implemented. Our analysis suggests that the chl a criterion of 10 μg L^{− 1} is achievable, but the water quality setting process should explicitly accommodate the natural variability by allowing for a realistic percentage of violations, e.g., exceedences of less than 10–15% of the weekly samples during the stratified period should still be considered as compliance of the system. The current epilimnetic total phosphorus criterion of 17 μg L^{− 1} is probably stringent and therefore a somewhat higher value (e.g., 20 μg L^{− 1}) may provide a more realistic goal. Phosphorus dynamics in the sediment–water column interface need to be revisited, as the internal nutrient loading can conceivably be a regulatory factor of the duration of the transient phase and the recovery resilience of the Harbour. We also pinpoint two critical aspects of the system dynamics that invite further investigation and will likely determine our predictive capacity to assess compliance with the chlorophyll a criterion of 10 μg L^{− 1}, i.e., the nutrient recycling mediated by the microbial food web and the structural shifts towards a zooplankton community dominated by large-sized and fast-growing herbivores. The latter prospect highlights the notion that the bottom-up (i.e., nutrient loading reduction) approach historically followed in the Harbour was sufficient to bring the system in its present state, but any further improvements should be viewed in the context of a combined bottom-up and top-down (i.e., alleviation of the zooplanktivorous pressure) control.     

The development of in vitro biocompatibility tests for the evaluation of intraocular biomaterials

Recent developments in ocular implant technology require the in vitro evaluation of ocular compatibility in early stage development programs. This requires an understanding and appreciation of the biological interactions which occur in the ocular environment and their relevance with respect to the clinical complications associated with surgical implantation of devices. This paper describes the development of a series of clinically reflective in vitro assays for assessing the potential ocular compatibility of novel intraocular lens materials. Staphylococcus epidermidis attachment, fibrinogen adsorption, mouse embryo fibroblast 3T3 adhesion and proliferation, primary rabbit lens cell adhesion, human peripheral blood macrophage adhesion and granulocyte activation tests were employed to evaluate two widely used intraocular biomaterials poly(methyl methacrylate) (PMMA) and silicone, and a novel biomimetic phosphorylcholine-based coating (PC). The performance of these materials in the in vitro assays was compared to their ability to reduce postoperative inflammation in vivo in a rabbit model. The results demonstrated that the in vitro assays described here are predictive of in vivo ocular compatibility. These assays offer a more relevant means of assessing the ocular compatibility of biomaterials than those presently required by the authorities for regulatory approval of medical devices and implants. © 1999 Kluwer Academic Publishers     

Biomineralized and chemically synthesized magnetic nanoparticles: A contrast

Magnetic nanoparticles (MNPs) have widely been synthesized through chemical processes for biomedical applications over the past few decades. Recently, a new class of MNPs, known as bacterial magnetosomes, has been isolated from magnetotactic bacteria, a natural source. These magnetosomes are magnetite or greigite nanocrystals which are biomineralized in the bacterial cell and provide magnet-like properties to it. Contrary to MNPs, bacterial magnetosomes are biocompatible, lower in toxicity, and can be easily cleared from the body due to the presence of a phospholipid bilayer around them. They also do not demonstrate aggregation, which makes them highly advantageous. In this review, we have provided an in-depth comparative account of bacterial magnetosomes and chemically synthesized MNPs in terms of their synthesis, properties, and biomedical applications. In addition, we have also provided a contrast on how magnetosomes might have the potential to successfully substitute synthetic MNPs in therapeutic and imaging applications.     

Changes in reflectin protein phosphorylation are associated with dynamic iridescence in squid

Many cephalopods exhibit remarkable dermal iridescence, a component of their complex, dynamic camouflage and communication. In the species Euprymna scolopes, the light-organ iridescence is static and is due to reflectin protein-based platelets assembled into lamellar thin-film reflectors called iridosomes, contained within iridescent cells called iridocytes. Squid in the family Loliginidae appear to be unique in which the dermis possesses a dynamic iridescent component with reflective, coloured structures that are assembled and disassembled under the control of the muscarinic cholinergic system and the associated neurotransmitter acetylcholine (ACh). Here we present the sequences and characterization of three new members of the reflectin family associated with the dynamically changeable iridescence in Loligo and not found in static Euprymna iridophores. In addition, we show that application of genistein, a protein tyrosine kinase inhibitor, suppresses ACh- and calcium-induced iridescence in Loligo. We further demonstrate that two of these novel reflectins are extensively phosphorylated in concert with the activation of iridescence by exogenous ACh. This phosphorylation and the correlated iridescence can be blocked with genistein. Our results suggest that tyrosine phosphorylation of reflectin proteins is involved in the regulation of dynamic iridescence in Loligo.