The effect of red pigment on the amyloidization of yeast proteins

The intensity of amyloid-bound thioflavine T fluorescence was studied in crude lysates of yeast strains carrying mutations in the ADE1 or ADE2 genes and accumulating the red pigment (a result of polymerization of aminoimidazoleribotide), and in white isogenic strains--either adenine prototrophs or carrying mutations at the first stages of purine biosynthesis. We found that the red pigment leads to a drop of amyloid content. This result, along with the data on separation of protein polymers of white and red strains in PAGE, suggests that the red pigment inhibits amyloid fibril formation. The differences in transmission of the thioflavine T fluorescence pattern by cytoduction and in blot-hybridization of pellet proteins of red and white [PSI(+) ] strains with Sup35p antibodies confirmed this conclusion. Purified red pigment treatment also led to a decrease of fluorescence intensity of thioflavine T bound to insulin fibrils and to yeast pellet protein aggregates from [PSI(+) ] strains. This suggests red pigment interaction with amyloid fibrils. Comparison of pellet proteins from red and white isogenic strains separated by 2D-electrophoresis followed by MALDI analysis has allowed us to identify 48 pigment-dependent proteins. These proteins mostly belong to functional classes of chaperones and proteins involved in glucose metabolism, closely corresponding to prion-dependent proteins that we characterized previously. Also present were some proteins involved in stress response and proteolysis. We suppose that the red pigment acts by blocking certain sites on amyloid fibrils that, in some cases, can lead in vivo to interfere with their contacts with chaperones and the generation of prion seeds.     

Small RNA F6 Provides Mycobacterium smegmatis Entry into Dormancy

Regulatory small non-coding RNAs play a significant role in bacterial adaptation to changing environmental conditions. Various stresses such as hypoxia and nutrient starvation cause a reduction in the metabolic activity of Mycobacterium smegmatis, leading to entry into dormancy. We investigated the functional role of F6, a small RNA of M. smegmatis, and constructed an F6 deletion strain of M. smegmatis. Using the RNA-seq approach, we demonstrated that gene expression changes that accompany F6 deletion contributed to bacterial resistance against oxidative stress. We also found that F6 directly interacted with 5′-UTR of MSMEG_4640 mRNA encoding RpfE2, a resuscitation-promoting factor, which led to the downregulation of RpfE2 expression. The F6 deletion strain was characterized by the reduced ability to enter into dormancy (non-culturability) in the potassium deficiency model compared to the wild-type strain, indicating that F6 significantly contributes to bacterial adaptation to non-optimal growth conditions.     

Genomic Regions Associated with Fusarium Wilt Resistance in Flax

Modern flax cultivars are susceptible to many diseases; arguably, the most economically damaging of these is the Fusarium wilt fungal disease. Over the past decades international flax breeding initiatives resulted in the development of resistant cultivars. However, much remains to be learned about the mechanisms of resistance to Fusarium infection in flax. As a first step to uncover the genetic factors associated with resistance to Fusarium wilt disease, we performed a genome-wide association study (GWAS) using 297 accessions from the collection of the Federal Research Centre of the Bast Fiber Crops, Torzhok, Russia. These genotypes were infected with a highly pathogenic Fusarium oxysporum f.sp. lini MI39 strain; the wilt symptoms were documented in the course of three successive years. Six different single-locus models implemented in GAPIT3 R package were applied to a selected subset of 72,526 SNPs. A total of 15 QTNs (Quantitative Trait Nucleotides) were detected during at least two years of observation, while eight QTNs were found during all three years of the experiment. Of these, ten QTNs occupied a region of 640 Kb at the start of chromosome 1, while the remaining QTNs mapped to chromosomes 8, 11 and 13. All stable QTNs demonstrate a statistically significant allelic effect across 3 years of the experiment. Importantly, several QTNs spanned regions that harbored genes involved in the pathogen recognition and plant immunity response, including the KIP1-like protein (Lus10025717) and NBS-LRR protein (Lus10025852). Our results provide novel insights into the genetic architecture of flax resistance to Fusarium wilt and pinpoint potential candidate genes for further in-depth studies.     

Planar defect of the nano-structured zinc oxide as the site for stabilization of the copper active species in Cu/ZnO catalysts

The catalytic activity of CuZn catalysts in the synthesis of methanol is related to those reduced Cu species, which originate from the Cu_xZn_1−xO solid solution of wurtzite-like structure. Copper cations in the Cu_xZn_1−xO solid solution are localized in the extended stacking faults of the ZnO lattice. Copper sites could be supposedly described as the product of introducing (OH)Cu(OH) to the planar defects of zinc oxide structure. Hydroxyl groups stabilize the planar defects of ZnO. The process of the samples reduction leads to the formation of flat Cu⁰ particles over the surface of zinc oxide. The planar defects of ZnO structure are preserved in the reduced state. During the reoxidation, copper atoms return back to the extended stacking faults of ZnO as the tape-like clusters of flat-square coordinated copper cations.     

Surface periodic domain structures for waveguide applications

We report the results of fabrication and investigations of surface periodic domain structures created by a set of quasi-point e-beam irradiations both on the Y- and X-cuts of LiNbO(3), and on Ti:LiNbO(3) and Zn:LiNbO(3) planar waveguides. Domain gratings with spatial periods from 4.75 to 7.25 μm were formed by a 25-keV e-beam. Doses from 500 to 2000 μC/cm(2) were used for different structures to estimate optimal fabrication conditions. The investigations allowed the visualization of the formed surface domain structures, estimation of their uniformity, and determination of waveguide generation of the second optical harmonic. The surface structures can be used in optical devices for the realization of quasi-phase-matched frequency conversion, which includes the creation of compact radiation sources based on waveguides.     

Computer Simulation of Decomposition Mechanisms for CL‐20, Hydrazine,and Their Binary System

An approach to simulate thermal destruction processes of energetic materials has been developed. It is based on the classification of structural features for nitro compounds and the experimental data regarding their decomposition mechanisms. This approach consists of the mathematical simulation of thermal decomposition mechanisms to predict the likely reactions that may occur during the destruction of organic compounds. On the basis of contemporary experimental data on the decomposition of energetic materials from various chemical classes, a set of semi‐empirical rules for modeling possible reaction pathways has been formulated. These rules allow the generation of a whole set of possible decomposition mechanisms for substances at different steps of their destruction. In this study, the suggested methodology is applied to 2,4,6,8,10,12‐hexanitro‐2,4,6,8,10,12‐hexaazaisowurtzitane (CL‐20) and hydrazine and to their binary energetic mixture. It has been shown that thermal decomposition of the binary system consists in decompositions of the separate compounds at the initial stage with subsequent collisions and interactions of the resultant intermediates and decomposition products at different stages. In addition to some experimentally found decomposition products, our simulation showed some other possible compounds among the final products.     

Cyanides,Arsenic, and Noble Metals in Abandoned Gold Ore Cyanidation Tailings and Surface Waters in a Permafrost Region (Transbaikal Territory,Russia)

Mine Water and the Environment - Geochemical and geophysical investigations were performed in an area of the Darasun ore cyanidation tailings impoundment, which is located in a permafrost region....