RNA splicing of bacterial genes in eukaryotes

The presence of intervening sequences or introns in eukaryotic genes has been known for more than 20 years, and the mechanisms underlying RNA splicing have been studied in depth both genetically and biochemically. In recent years, however, an increasing number of bacterial genes have been introduced into higher eukaryotes as important tools for genetic studies. Their gene products are frequently used as an indirect measure for cell type-specific promoter activity, as, for example, in the case of chloramphenicol acetyl transferase (CAT assay) or beta-galactosidase. Here we show that RNA splicing of two prokaryotic genes encoding site-specific DNA recombinases occurs in eukaryotic cells. In one case, splicing is only observed after treatment of cells with the cytokine alpha interferon. We further demonstrate that mutating an intragenic donor splice site in a bacterial gene apparently activates a second, alternative splicing pathway. In conjunction with previous reports, our findings should also be regarded as a warning that splicing of bacterial genes in higher eukaryotes is a more common phenomenon than presently recognized, which may be difficult to overcome and may cause problems in the interpretation of experimental results.     

高码率DQPSK遥测信号解调方法研究

本文根据高码速率 DQPSK遥测信号的特点 ,介绍了一种通过数字瞬时测频来完成对高码速率 DQPSK信号解调的方法。该方法算法简单 ,处理速度快 ,误码率低 ,能够有效地抑制多普勒频率对解调的影响 ,便于数字信号处理芯片硬件实现。同时给出了解调的仿真结果     

测量光纤放大器小信号增益谱的一种简便方法

提出了一种用小功率宽带光源较精确测量光纤放大器小信号增益谱的方法。数值模拟表明 ,该方法是完全可行的 ,并且实验结果与数值模拟结果一致。该方法具有简便、易操作的特点。     

Does precontemplation represent a homogeneous stage category? A latent class analysis on German smokers.

The authors examined the subtype structure of smokers classified in the precontemplation stage of change within the transtheoretical model. From a general practice-based sample of 1,499 daily smoking patients from Germany (participation rate 80%), they used a subgroup of 929 smokers who were classified in the precontemplation stage and applied latent class analysis, using the pros and cons of nonsmoking and smoking cessation self-efficacy as the defining variables. Cross-sectional validation of the emerging classes was based on smoking behavior and processes of change variables. For longitudinal validation, generalized estimation equation analyses were used on motivational and abstinence criteria from 6-, 12-, 18-, and 24-month follow-ups. A 4-class model best represented the data. Three subtypes (labeled progressive, immotive, and disengaged pessimistic) were similar to clusters identified in U.S. studies. The 4th (disengaged optimistic), by contrast, was reminiscent of a type that had previously only emerged in a Dutch study. Cross-sectional and longitudinal validation results confirmed the distinctiveness and predictive power of the classes. The findings highlight the importance of tailoring interventions for smoking behavior change to the needs of different subgroups of precontemplating smokers. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

二相与四相无限折叠混沌扩频序列的相关特性分析

本文利用无限折叠迭代混沌映射产生二相与四相混沌扩频序列。分析了序列轨迹点具有的分布律与相关特性,模拟结果表明它们分别能获得比同等长度的二相与四相Logistic序列、四相Chebyshev序列、m序列、Gold序列更好的自相关与互相关性能,有利于解决CDMA移动通信中对相关性要求很严格的抗多址（多径）干扰、多用户检测等关键问题。     

Risk-based rules for crane safety systems

The International Maritime Organisation (IMO) has recommended a method called formal safety assessment (FSA) for future development of rules and regulations. The FSA method has been applied in a pilot research project for development of risk-based rules and functional requirements for systems and components for offshore crane systems. This paper reports some developments in the project. A method for estimating target reliability for the risk-control options (safety functions) by means of the cost/benefit decision criterion has been developed in the project and is presented in this paper. Finally, a structure for risk-based rules is proposed and presented.     

一台高强化柴油机排气噪声有源控制实验研究

柴油机噪声使操作人员工作环境恶化,长期还危害操作人员的身体健康。排气噪声对柴油机整机噪声贡献很大,本文设计了一套自适应有源噪声控制（Adaptive Active Noise Control）系统用于控制排气噪声,实验结果表明该系统效果良好。     

金刚石膜的计算机虚拟制备技术中的分子动力学模拟

综述了近年来金刚石薄膜形成过程的分子动力学（Molecular Dynamics,简称MD）模拟研究,详细地阐述了原子间相互作用势的选取,总结了不同沉积条件下MD的计算模型和几种典型情况下的模拟结果。研究表明：在原子尺度上,MD方法能较全面地提供有关膜生长的信息,对进一步了解金刚石膜形成的微观机制以及为细观层次仿真提供基本信息均具有重要意义。     

液体低温热导率的测试

介绍了非稳态法测试液体在低温下（－１００～５０℃）的热导率的测试原理、方法，测试了全氟三乙胺的低温热导率。为了提高测试的重复性，采用了相对比较法，并给出了测试装置及测试结果和误差分析。