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排序方式: 共有2610条查询结果,搜索用时 15 毫秒
31.
Romina Romaniello Ludovica Pasca Elena Panzeri Fulvio DAbrusco Lorena Travaglini Valentina Serpieri Sabrina Signorini Chiara Aiello Enrico Bertini Maria Teresa Bassi Enza Maria Valente Ginevra Zanni Renato Borgatti Filippo Arrigoni 《International journal of molecular sciences》2022,23(12)
The inositol 1,4,5-triphosphate receptor type 1 (ITPR1) gene encodes an InsP3-gated calcium channel that modulates intracellular Ca2+ release and is particularly expressed in cerebellar Purkinje cells. Pathogenic variants in the ITPR1 gene are associated with different types of autosomal dominant spinocerebellar ataxia: SCA15 (adult onset), SCA29 (early-onset), and Gillespie syndrome. Cerebellar atrophy/hypoplasia is invariably detected, but a recognizable neuroradiological pattern has not been identified yet. With the aim of describing ITPR1-related neuroimaging findings, the brain MRI of 14 patients with ITPR1 variants (11 SCA29, 1 SCA15, and 2 Gillespie) were reviewed by expert neuroradiologists. To further evaluate the role of superior vermian and hemispheric cerebellar atrophy as a clue for the diagnosis of ITPR1-related conditions, the ITPR1 gene was sequenced in 5 patients with similar MRI pattern, detecting pathogenic variants in 4 of them. Considering the whole cohort, a distinctive neuroradiological pattern consisting in superior vermian and hemispheric cerebellar atrophy was identified in 83% patients with causative ITPR1 variants, suggesting this MRI finding could represent a hallmark for ITPR1-related disorders. 相似文献
32.
David Bravo Katherine Zepeda-Morales Carola J. Maturana Jeffri S. Retamal Alejandro Hernndez Teresa Pelissier Rafael Barra Patricio Sez-Briones Hctor Burgos Luis Constandil 《International journal of molecular sciences》2022,23(12)
Pannexin 1 (Panx1) is involved in the spinal central sensitization process in rats with neuropathic pain, but its interaction with well-known, pain-related, ligand-dependent receptors, such as NMDA receptors (NMDAR) and P2X7 purinoceptors (P2X7R), remains largely unexplored. Here, we studied whether NMDAR- and P2X7R-dependent nociceptive signaling in neuropathic rats require the activation of Panx1 channels to generate spinal central sensitization, as assessed by behavioral (mechanical hyperalgesia) and electrophysiological (C-reflex wind-up potentiation) indexes. Administration of either a selective NMDAR agonist i.t. (NMDA, 2 mM) or a P2X7R agonist (BzATP, 150 μM) significantly increased both the mechanical hyperalgesia and the C-reflex wind-up potentiation, effects that were rapidly reversed (minutes) by i.t. administration of a selective pannexin 1 antagonist (10panx peptide, 300 μM), with the scores even reaching values of rats without neuropathy. Accordingly, 300 μM 10panx completely prevented the effects of NMDA and BzATP administered 1 h later, on mechanical hyperalgesia and C-reflex wind-up potentiation. Confocal immunofluorescence imaging revealed coexpression of Panx1 with NeuN protein in intrinsic dorsal horn neurons of neuropathic rats. The results indicate that both NMDAR- and P2X7R-mediated increases in mechanical hyperalgesia and C-reflex wind-up potentiation require neuronal Panx1 channel activation to initiate and maintain nociceptive signaling in neuropathic rats. 相似文献
33.
Jeremy Rott Eva Teresa Toepfer Maria Bartosova Ana Kolevica Alexander Heuser Michael Rabe Geert Behets Patrick C. DHaese Viktoria Eichwald Manfred Jugold Ivan Damgov Sotirios G. Zarogiannis Rukshana Shroff Anton Eisenhauer Claus Peter Schmitt 《International journal of molecular sciences》2022,23(14)
Serum calcium isotopes (δ44/42Ca) have been suggested as a non-invasive and sensitive Ca balance marker. Quantitative δ44/42Ca changes associated with Ca flux across body compartment barriers relative to the dietary Ca and the correlation of δ44/42CaSerum with bone histology are unknown. We analyzed Ca and δ44/42Ca by mass-spectrometry in rats after two weeks of standard-Ca-diet (0.5%) and after four subsequent weeks of standard- and of low-Ca-diet (0.25%). In animals on a low-Ca-diet net Ca gain was 61 ± 3% and femur Ca content 68 ± 41% of standard-Ca-diet, bone mineralized area per section area was 68 ± 15% compared to standard-Ca-diet. δ44/42Ca was similar in the diets, and decreased in feces and urine and increased in serum in animals on low-Ca-diet. δ44/42CaBone was higher in animals on low-Ca-diet, lower in the diaphysis than the metaphysis and epiphysis, and unaffected by gender. Independent of diet, δ44/42CaBone was similar in the femora and ribs. At the time of sacrifice, δ44/42CaSerum inversely correlated with intestinal Ca uptake and histological bone mineralization markers, but not with Ca content and bone mineral density by µCT. In conclusion, δ44/42CaBone was bone site specific, but mechanical stress and gender independent. Low-Ca-diet induced marked changes in feces, serum and urine δ44/42Ca in growing rats. δ44/42CaSerum inversely correlated with markers of bone mineralization. 相似文献
34.
Júlia Faura Laura Ramiro Alba Simats Feifei Ma Anna Penalba Teresa Gasull Anna Rosell Joan Montaner Alejandro Bustamante 《International journal of molecular sciences》2022,23(15)
After stroke and other brain injuries, there is a high incidence of respiratory complications such as pneumonia or acute lung injury. The molecular mechanisms that drive the brain-lung interaction post-stroke have not yet been elucidated. We performed transient middle cerebral artery occlusion (MCAO) and sham surgery on C57BL/6J mice and collected bronchoalveolar lavage fluid (BALF), serum, brain, and lung homogenate samples 24 h after surgery. A 92 proteins-panel developed by Olink Proteomics® was used to analyze the content in BALF and lung homogenates. MCAO animals had higher protein concentration levels in BALF than sham-controls, but these levels did not correlate with the infarct volume. No alteration in alveolar-capillary barrier permeability was observed. A total of 12 and 14 proteins were differentially expressed between the groups (FDR < 0.1) in BALF and lung tissue homogenates, respectively. Of those, HGF, TGF-α, and CCL2 were identified as the most relevant to this study. Their protein expression patterns were verified by ELISA. This study confirmed that post-stroke lung damage was not associated with increased lung permeability or cerebral ischemia severity. Furthermore, the dysregulation of HGF, TGF-α, and CCL2 in BALF and lung tissue after ischemia could play an important role in the molecular mechanisms underlying stroke-induced lung damage. 相似文献
35.
36.
Nicolette Pegels Isabel González Irene Martín María Rojas Teresa García Rosario Martín 《Food Control》2011,22(8):1189-1196
A TaqMan real-time polymerase chain reaction (PCR) method was developed for specific detection of bovine, ovine and caprine processed animal protein (PAP) in industrial feedstuffs. The method uses species-specific primers and probes targeting short mitochondrial D-loop sequences, and a positive amplification control based on 18S rRNA gene. The applicability of the real-time PCR protocol was assessed through analysis of 126 industrial feed samples that were manufactured to reproduce rendering processes of commercial feeds destined for farmed animals. The assay successfully classified samples as positive or negative according to the ruminant composition, enabling qualitative detection of banned material in feeds at levels as low as 0.1%. Although the method provides quantitative potential, results suggest that the real quantitative capability of the assay is limited by the existing variability in terms of composition and processing treatments of the feeds, which affect the amount and quality of amplifiable DNA. 相似文献
37.
公共和私营部门在标准化领域的合作是非常有效且有益的。当此类合作跨越国界时,知识的获得就会更加广泛,得到的回报也就更多样了。ASTM国际标准组织目前举办了民用核电强化培训课程,最值得一提的当属韩国标准协会及其业界的培训。培训资金主要来源于韩国政府,还有一部分支持来自业界。此次培训项目是一个成功的典范,ASTM负责后勤保障和所培训的内容。 相似文献
38.
Electric arc furnace dusts are among the most environment polluting wastes. Numerous utilization technologies have been developed for dusts containing up to 4% of Zn and more than 20% of Zn. However, the remaining part of steelmaking dusts are the most problematic ones, as they are mostly dumped generating costs and posing serious environmental threats. This paper provides an analysis of the potential utilization options for dusts containing ca. 10% of zinc generated in a single electric steelmaking shop. Physical and chemical properties of dusts have been determined and examined, and furthermore, results of the studies on the EAFD utilization in production of cement clinker and industrial glass have been discussed. It has been found that a particularly beneficial feature of the production technology proposed is that the iron content exceeds 30%. The influence of the dusts used on the functional properties of the products obtained has been established as well as the environmental impact of the processes and products in question and the dust mass possible to be utilized in the production technology proposed have been determined. 相似文献
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