Bioglass as a carrier for reindeer bone protein extract in the healing of rat femur defect

Bioactive glasses have been developed as scaffolds for bone tissue engineering but combination with reindeer bone protein extract has not been evaluated. We investigated the effects of bone protein extract implants (5–40 mg dosages) with bioglass (BG) carrier on the healing of rat femur defects. Bioglass implants and untreated defects served as controls. All doses of extract increased bone formation compared with the control groups, and bone union was enhanced with doses of 10 mg or more. In comparison with untreated defect, mean cross-sectional bone area at the defect site was greater when implants with BG + 15 mg of extract or bioglass alone were used, bone density at the defect site was higher in all bioglass groups with and without bone extract, and the BG + 15 mg extract dosage marginally increased bone torsional stiffness in mechanical testing. Bioglass performed well as a carrier candidate for reindeer bone protein extract.     

Influence of Zn on the oxide layer on AISI 316L(NG) stainless steel in simulated pressurised water reactor coolant

The oxidation of AISI 316L(NG) stainless steel in simulated pressurised water reactor (PWR) coolant with or without addition of 1 ppm Zn at 280 °C for up to 96 h has been characterised in situ by electrochemical impedance spectroscopy (EIS), both at the corrosion potential and under anodic polarisation up to 0.5 V vs. the reversible hydrogen electrode (RHE). Additional tests were performed in simulated PWR coolant with the addition of 0.01 M Na₂B₄O₇ to exclude the effect of pH excursions probably due to Zn hydrolysis reactions. The thickness and in-depth composition of the oxide films formed at open circuit and at 0.5 V vs. RHE in the investigated electrolytes have been estimated from X-ray photoelectron spectroscopy (XPS) depth profiles. The kinetic and transport parameters characterising the oxide layer growth have been estimated using a calculational procedure based on the mixed conduction model for oxide films. Successful simulations of both the EIS and XPS data have been obtained. The parameter estimates are discussed in terms of the effect of Zn on the oxide layers on stainless steel in PWR conditions.     

Study of Interatomic Potentials in ZnS—Crystal-GRID Experiments Versus Ab Initio Calculations

Crystal-GRID measurements have been performed with ZnS single crystals. For the first time, an asymmetric Crystal-GRID line shape could be observed. The preliminary data evaluation indicates that the reported lifetime of the 3221 keV level in ³³S is too short. A value of about 60 fs has been found. Due to this “long” lifetime the line shape is much less structured than calculated with the reported lifetime.     

Reconfiguring flexible machine modules of a PCB assembly line

Assembly of electronic components to Printed Circuit Boards (PCB) is a complicated manufacturing process and therefore its control is usually divided into several subtasks which are handled separately. We consider the combined task of determining a machine configuration and line balancing for a single assembly line of interconnected, reconfigurable machine modules and one PCB type in production. The modules can be tailored to the needs of each PCB type by suitable assignments for placement heads, nozzles and feeders. Out of these, the component-to-machine assignment appears to be most difficult and we propose five different solution methods for it; brute force, random, greedy, local search and genetic algorithm. The genetic algorithm outperformed the other methods in practical test.     

An impedance-based predictive control strategy for the state-of-health of PEM fuel cell stacks

This work presents a control strategy for PEM fuel cell systems based on simultaneous impedance measurements on single cells. This control strategy distinguishes between flooding and drying of the cells in a stack and helps to run the stack at an optimal operating point. In the presented experiments, it has been found that impedance measurements can detect flooding phenomena in single cells minutes before they can be seen in related polarisation curves. It is shown that impedance measurements at two specific frequencies, one high and one low frequency impedance, are sufficient to predict voltage drops caused by flooding and drying. In flooding mode, the imaginary part of the low frequency impedance changes while the high frequency impedance remains stable and vice versa in drying mode. This technique reduces measuring time compared to the measurement of whole impedance spectra, without losing important information for the control of the system.     

Compressional Behaviour of an Agglomerated Cellulose Powder

The ability of an agglomerated cellulose powder to total and plastic deformation was evaluated and compared with those of Avicel PH 101, Emcocel and an experimental depolymerized cellulose powder. The elastic recovery of compressed cellulose tablets was also measured. The effects of deformation of the material during the tableting process and recovery of tablet after maximum compression on the mechanical strength of tablets were also discussed.

The apparent net work done into tablets during compression as well as the yield pressures to total and plastic deformation, determined from the Heckel treatment, showed no great differences between the agglomerated cellulose powder and the other cellulose powders. Thus all the cellulose materials studied had rather similar ability to total, i.e. elastic and plastic, deformation and to permanent, i.e. pure plastic, deformation. The obvious fragmentation of the agglomerated cellulose powder already at low compressional pressure, however, seemed to be advantageous for the formation of strong compacts.

Both rapid and total elastic recovery of compressed cellulose tablets showed clear differences between the cellulose materials and these differences correlated with the previously measured strength of cellulose tablets. The agglomerated cellulose powder had the smallest tendency to both kind of elastic recoveries of tablets. Obviously, due to the large interparticle contact areas, the ability of this material to establish more bonds between adjacent particles during compression was greater than those of other celluloses. The elastic recovery was greatest for depolymerized cellulose tablets indicating the poorest binding ability of the particles of this material.     

Specifying Cash-Point with DisCo

We describe how the cash-point service problem of Formal Methods ’99 is specified using DisCo [Dis, JKS91]. Received January 2000 / Accepted in revised form December 2000     

One Question,Multiple Answers: Biochemical and Biophysical Screening Methods Retrieve Deviating Fragment Hit Lists

Fragment‐based lead discovery is gaining momentum in drug development. Typically, a hierarchical cascade of several screening techniques is consulted to identify fragment hits which are then analyzed by crystallography. Because crystal structures with bound fragments are essential for the subsequent hit‐to‐lead‐to‐drug optimization, the screening process should distinguish reliably between binders and non‐binders. We therefore investigated whether different screening methods would reveal similar collections of putative binders. First we used a biochemical assay to identify fragments that bind to endothiapepsin, a surrogate for disease‐relevant aspartic proteases. In a comprehensive screening approach, we then evaluated our 361‐entry library by using a reporter‐displacement assay, saturation‐transfer difference NMR, native mass spectrometry, thermophoresis, and a thermal shift assay. While the combined results of these screening methods retrieve 10 of the 11 crystal structures originally predicted by the biochemical assay, the mutual overlap of individual hit lists is surprisingly low, highlighting that each technique operates on different biophysical principles and conditions.