Dynamics of control on digital platforms

Digital platforms are supraorganizational entities that use digital technology to facilitate interactions between diverse actors, leading to novel forms of organisation and accompanying forms of control. The current Information Systems (IS) literature, however, struggles to describe control on digital platforms in a way that does justice to the dynamic character of the phenomenon. Taking this as an opportunity, we follow the enactment of control over time and across parties in a hybrid ethnographic study of the social commerce platform Poshmark. Specifically, we conceptualise the dynamics of control as changes in the means of control—formal or informal—and the sources of control—operator or participants—over time. Tracking these conceptual dimensions, we identify the distinct ways control has changed on Poshmark. Synthesising these findings into four dynamics of control, we show that control on digital platforms is rarely static due to aggregate effects arising from the operator and from participant interactions with each other through the digital features deployed on the platform. Based on these insights, our study contributes to the IS literature on control by broadening the conception of control on digital platforms. The theoretical and practical insights generated in this paper thereby lay the foundation for the systematic study of the dynamics of control that are unique to platform environments.     

Tuning Nanobodies’ Bioactivity: Coupling to Ultrasmall Gold Nanoparticles Allows the Intracellular Interference with Survivin

Nanobodies are highly affine binders, often used to track disease-relevant proteins inside cells. However, they often fail to interfere with pathobiological functions, required for their clinical exploitation. Here, a nanobody targeting the disease-relevant apoptosis inhibitor and mitosis regulator Survivin (SuN) is utilized. Survivin's multifaceted functions are regulated by an interplay of dynamic cellular localization, dimerization, and protein–protein interactions. However, as Survivin harbors no classical “druggable” binding pocket, one must aim at blocking extended protein surface areas. Comprehensive experimental evidence demonstrates that intracellular expression of SuN allows to track Survivin at low nanomolar concentrations but failed to inhibit its biological functions. Small angle X-ray scattering of the Survivin-SuN complex locates the proposed interaction interface between the C-terminus and the globular domain, as such not blocking any pivotal interaction. By clicking multiple SuN to ultrasmall (2 nm) gold nanoparticles (SuN-N), not only intracellular uptake is enabled, but additionally, Survivin crosslinking and interference with mitotic progression in living cells are also enabled. In sum, it is demonstrated that coupling of nanobodies to nanosized scaffolds can be universally applicable to improve their function and therapeutic applicability.