A high sucrose diet decreases the mechanical strength of bones in growing rats

High sucrose diets alter mineral metabolism in humans and animals. We examined the effect of a high sucrose diet on bone growth, composition and mechanical strength in growing rats. Weanling Wistar rats received a high sucrose (43 g/100 g) diet (9 males, 11 females). In the control diet (8 males, 8 females), sucrose was replaced with potato starch, providing an equal energy value. At the onset of the experiment, bones were marked by tetracycline. After 5 wk, the tibias and femurs were weighed, and maximum breaking strengths were determined. Tibias were cut at the tibia-fibular junction; the widths of the bone at the start of the experiment, the periosteal bone formation during the experiment, the widths of the medullary cavity and the final bone width were determined from tetracycline lines. Bone ash weight, Ca and P contents were determined. The breaking strengths of both bones were significantly lower in the sucrose-fed groups of both sexes. In females, the weight of both bones and the final width of the tibias were significantly lower in the sucrose-fed group. The Ca concentration in both bones and the P concentration in tibias were significantly lower in the sucrose-fed group. It was concluded that the metabolic interference induced by sucrose was the reason for the differences. The alterations were more pronounced in females, but independent of body weight.     

Group learning in medical education]

The implementation of group learning in medical education puts special demands on the participants, but also offers pedagogical benefits, which may be conductive to the efficient learning of facts, skills and attitudes. The group makes it possible to verify or modify the learned material, which is an important part of preparing for a career as a doctor. Students develop a dependence on others when solving problems in groups, whereas as clinicians they are often alone when confronted with problems demanding quick decisions. In this article we discuss some of the pedagogical principles involved in group learning and make suggestions on how to apply them to learning based on problem solving and other forms of group learning in the medical curriculum.     

The medullary dorsal reticular nucleus facilitates pain behaviour induced by formalin in the rat

The influence of the dorsal reticular nucleus (DRt) on pain behaviour during the formalin test was studied in the rat by lesioning the nucleus through local application of electrical current or quinolinic acid. Animals in which the DRt was lesioned ipsilaterally to the paw injected with formalin spent less time in focused (licking, biting or scratching the injected paw) and total (focused pain behaviour plus protection of the injected paw during movements) pain behaviour, and showed paw-jerks less frequently than non-lesioned animals in both phases 1 and 2 of the test. Animals in which the DRt was lesioned contralaterally to the injected paw presented a decrease in total pain behaviour and number of paw-jerks only during phase 2. The number of superficial (laminae I-II) and deep (laminae III-VI) spinal dorsal horn cells expressing the c-fos proto-oncogene 2 h after subcutaneous injection of formalin was reduced by 34% and 50%, respectively, in animals with an ipsilateral DRt lesion as compared to non-lesioned rats. No differences in c-fos expression were observed after lesioning the DRt contralateral to the formalin injection. The results indicate that the DRt is involved in the facilitation of nociception during the formalin test by enhancing the response capacity of dorsal horn neurons to noxious stimulation. It is suggested that the pronociceptive action of the DRt is mediated by the reciprocal connections it establishes with the spinal dorsal horn.     

The activation and function of host matrix metalloproteinases in dentin matrix breakdown in caries lesions

Matrix metalloproteinases (MMPs) are a family of enzymes which, in concert, are capable of degrading collagen. We investigated whether human MMPs could participate in the degradation of dentin organic matrix after demineralization. We performed Western blot analyses using MMP-specific antibodies to identify MMPs in human dental caries lesions. Enzymography and functional activity assays, with 125I-labeled gelatin as substrate or quantitating the degradation of type I collagen, were used to determine the activity of purified and salivary gelatinolytic (MMP-2 and MMP-9) and collagenolytic (MMP-8) enzymes with and without acid-activation in pHs relevant to caries. Respective analyses were done with caries-related bacteria. We performed electron microscope analyses to assess the degradative activity of sterilized salivary host MMPs on demineralized human dentin. Human MMP-2, MMP-8, and MMP-9 were identified in demineralized dentinal lesions. The latent purified forms of these enzymes were activated at low pH (4.5), followed by neutralization, mimicking the conditions during caries progression. Incubation of human saliva at low pH followed by neutralization resulted in a four-fold increase in the gelatinolytic activity. No gelatinolytic or collagenolytic activity was observed in bacterial samples. The activated enzymes in saliva degraded demineralized dentin organic matrix in vitro. These results demonstrate the pH-dependent activation mechanism of MMPs, which may have a distinct role in different physiological and pathological conditions. They further demonstrate that host MMPs, activated by bacterial acids, have a crucial role in the destruction of dentin by caries.     

Free vibrations of fluid-filled cylindrical shells on elastic foundations

The free vibration characteristics of fluid-filled cylindrical shells on elastic foundations are presented by a semi-analytical finite element method. A shell is discretized into cylindrical finite elements where shell governing equations based shape functions in the longitudinal direction are used instead of the usual simple polynomials. Non-uniformities of the foundations in the circumferential and longitudinal directions are handled by the Fourier series and an element mesh strategy, respectively. The fluid domain is described by the potential flow theory. The hydrodynamic pressure acting on shells is derived from the condition for dynamic coupling of the fluid-structure. The effect of fluid in a shell, shell geometries, and foundation parameters on the dynamic behavior of fluid-containing shells is investigated. Numerical results based on the present method converge more rapidly than those obtained by the simple polynomial formulation. The method is suitable for the problem considered due to its generality, simplicity, and potential for further development.     

K5公寓

这座公寓大楼位于GrünerlФkka,是最丰富多彩和引人注目的区域,被称之为奥斯陆城中的纽约。这块地是至今尚存的19世纪住房与小规模工业亲密交融的罕见典型,遍布着规则排列的窗户和阁楼。为此,新建筑也模仿了这一做法,只是在墙面上加以变化,从而与周围环境既形成了对比,又十分融洽。     

Drivers for retrofit: a sociocultural approach to houses and inhabitants

ABSTRACT

Private households constitute approximately 30% of the total energy consumption in Denmark. The greatest potential for reducing energy consumption in private households is through retrofits of existing houses. One of the most common ways to promote retrofits is the act of sending an energy advisor to visit homeowners in order to explain the technological and economic advantages that arise from undertaking a retrofit. However, little is known about how homeowners make sense of retrofits. Based on an extensive anthropological fieldwork study of 12 Danish homeowners who had received energy advice, retrofits were investigated from the perspective of the everyday life of homeowners. Three social dimensions that played an important role when the people in the study discussed house renovation were identified and discussed: houses as part of homeowners’ life cycles and personal events; houses as frameworks for social relations; and houses as markers of social values and status. These results indicate a need to develop new strategies to promote retrofits more effectively, which focus less on the techno-economic aspects and more on the social dimensions.