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11.
The model of complex formation between polypeptide adopted lefthanded conformation of polyproline II type and minor groove of B-DNA presented. The specific recognition is attained due to possibility of hydrogen bond between Gly NH-group and Thymine O2-group and in addition between Pro CO-group and Guanine NH2-group and between Gly CO-group and Guanine NH2-group. Stereochemical satisfactory of the complex is observed. 相似文献
12.
JF McCarthy DJ Cook MG Massad Y Sano KJ O''Malley NR Ratliff RW Stewart NG Smedira RC Starling JB Young PM McCarthy 《Canadian Metallurgical Quarterly》1998,14(2):197-200
28 isolates of canine parvovirus type-2 (CPV-2) were obtained from dogs with hemorrhagic gastroenteritis in Italy. The antigenic structure of CPV-2 isolates was characterized, using four discriminating monoclonal antibodies. In addition, four vaccinal strains were examined. Similar to reports from Australia and the United Kingdom, a much higher prevalence of CPV-2a (25/28 isolates) was observed than the other variant type, CPV-2b (3/28 isolates). DNA fragments (2.2 kbp) of representative strains of CPV-2, CPV-2a and CPV-2b were amplified by the polymerase chain reaction (PCR) and the products were digested by the restriction enzymes (RE) RsaI, HpaII, HindIII and PvuII. The RvaI enzyme allows the differentiation of CPV-2 from CPV-2a and CPV-2b. 相似文献
13.
G Nadali EA de Wynter F Perandin L Tavecchia C Vincenzi A Ambrosetti M Fornalè G Perona G Pizzolo NG Testa 《Canadian Metallurgical Quarterly》1998,83(12):1059-1065
BACKGROUND AND OBJECTIVE: Long-term culture-initiating cells (LTC-IC) are the best available approximation to an in vitro assay of stem cells in humans although they still represent a heterogeneous population in terms of proliferative capacity and sensitivity to different growth factors. Human umbilical cord blood (CB) is rich in hemopoietic progenitor cells, as measured by clonogenic assays and contains stem cells capable of reconstituting the marrow after ablation in clinical transplantation. We evaluated the influence of culture conditions on the in vitro behavior of LTC-IC from CB. DESIGN AND METHODS: LTC-IC were evaluated in long-term cultures, comparing two types of murine stromal cell lines: M2-10B4 and M2-10B4 transfected with cDNAs for human G-CSF and IL-3. RESULTS: Two and five fold higher numbers of terminally differentiated cells were produced during nine weeks of culture of CB mononuclear or CD34+ cells respectively, in cultures containing a M2-10B4 IL-3 G-CSF cell line compared to cultures containing the parental cell line. Likewise, a higher number of colony-forming cells (CFC) were detected in the supernatant of cultures with the transfected cell line. In contrast, the number of CFC generated within the stromal layer, after 5 or 9 weeks of culture, was significantly higher in cultures on M2-10B4 cells than those on M2-10B4 IL-3 G-CSF. INTERPRETATION AND CONCLUSIONS: Our results show that the proliferative capacity of CB LTC-IC can be strongly influenced by culture conditions and that the frequency of LTC-IC estimated using these cell lines as stromal support is not identical. 相似文献
14.
The response people have to vaccination varies because their immune systems differ and vaccine failures occur. Here we consider the effect that a random response, independent for each vaccinee, has on the vaccination coverage required to prevent epidemics in a large community. For a community of uniformly mixing individuals an explicit expression is found for the critical vaccination coverage (CVC) and the effect of the vaccine response is determined entirely by the mean E(AB), where A and B, respectively, reflect the infectivity and susceptibility of a vaccinated individual. This result shows that the usual concept of vaccine efficacy, which focuses on the amount of protection the vaccine provides the vaccinee against infection, is not adequate to describe the requirements for preventing epidemics when vaccination affect infectivity. The estimation of E(AB) poses a problem because A and B refer to the vaccine response of the same individual. Similar results are found when there are different types of individual, but now the mean E(AB) may differ between types. However, for a community made up of households it is shown that the CVC also depends on other characteristics of the vaccine response distribution. In practice this means that estimating a single measure of vaccine effectiveness is generally not enough to determine the CVC. For a specific community of households it is found that the vaccination coverage required to prevent epidemics decreases as the variation in the vaccine response increases. 相似文献
15.
R Kneen NG Pham T Solomon TM Tran TT Nguyen BL Tran J Wain NP Day TH Tran CM Parry NJ White 《Canadian Metallurgical Quarterly》1998,27(4):845-850
PURPOSE: To review the University of Florida experience in treating ependymomas, analyze prognostic factors, and provide treatment recommendations. METHODS AND MATERIALS: Forty-one patients with ependymoma and no metastases outside the central nervous system received postoperative radiotherapy with curative intent between 1966 and 1989. Ten patients had supratentorial lesions, 22 had infratentorial lesions, and 9 had spinal cord lesions. All patients had surgery (stereotactic biopsy, subtotal resection, or gross total resection). Most patients with high-grade lesions received radiotherapy to the craniospinal axis. Low-grade intracranial lesions received more limited treatment. Spinal cord lesions were treated using either partial spine or whole spine fields. RESULTS: Of 32 intracranial tumors, 21 recurred, all at the primary site; no spinal cord tumors recurred. Overall 10-year survival rates were 51% (absolute) and 46% (relapse-free); by tumor site: spinal cord, 100%; infratentorial, 45%; supratentorial, 20% (p = 0.002). On multivariate analysis, tumor site was the only factor that influenced absolute survival (p = 0.0004); other factors evaluated included grade, gender, age, duration of symptoms, resection extent, primary tumor dose, treatment field extent, surgery-to-radiotherapy interval, and days under radiotherapy treatment. CONCLUSIONS: Patients with supratentorial or infratentorial tumors receive irradiation, regardless of grade. Craniospinal-axis fields are used when spinal seeding is radiographically or pathologically evident. Spinal cord tumors are treated using localized fields to the primary site if not completely resected. Failure to control disease at the primary site remains the main impediment to cure. 相似文献
16.
LIU Dacheng CAO Chunan WANG Fuhui LOU Hanyi ZHA NG Lixin Institute of Corrosion Protection of Metals Academia Sinica Shenyang ChinaOriginally published in ACTA METALL SIN 《金属学报(英文版)》1989,2(8):143-145
The resistances against localized corrosion of 1Cr18Ni9Ti microcrystals with normal grainsize and bcc structure obtained hy magnetron sputtering have been compared.The two kindsof microcrystals with (110) and (211) textures respectively were obtained under differentsputtering conditions.Both microcrystals were found to have better resistance against local-ized corrosion than the crystal with normal grain.The microcrystal with (110) preferredorientation has larger resistance to localized corrosion than that with (211) preferredorientation. 相似文献
17.
18.
高速公路事件检测是交通信息工程及控制学科中的一项重要研究课题,以高速公路交通流的特点为研究对象,提出了一种基于支持向量机(SVM)的高速公路事件检测算法.根据支持向量机的基本原理,分别设计了基于线性不可分SVM、齐次多项式核函数、高斯径向基核函数、双曲线正切核函数等不同核函数的事件检测算法.仿真结果表明:针对不同的交通流状况,选择合适的SVM模型和核函数,得到的检测结果与经典的加利福尼亚算法相比,检测效率高,性能指标好,具有较高的实际应用价值. 相似文献
19.
为了有效地评估客户的可信度,提高信贷机构经济效益。文中通过统计学中的参数方法Logistic回归和非参数方法分类树这两种方法,建立两种模型对数据进行预测,应用SPSS软件的Binary Logistic Regression方法,利用ROC曲线的性质来对模型的性能进行评价,根据输出结果比较两种模型在应用中都是可行有效的,在实际操作中应因地制宜,把握两种方法的优势,得到更有价值的结果. 相似文献
20.
Subdermal superficial liposuction, first presented by the authors at the ISAPS Congress at Zurich in 1989, is performed with thin three-hole Mercedes cannulas (diameter ranges from 1.8 to 2 mm) to treat small and secondary adiposities and to allow better skin retraction. Suction of the subdermal layer of fat reduces the thickness and consistency of the superficial fat and enhances the possibility of skin retraction. In cases where there is a large adiposity of the abdomen, arms, or inner thighs, there is a conspicuous volume of fat whose weight tends to overstretch and to carry the overlying skin downward. In these cases we need to reduce the large fat volume to permit effective skin retraction. Therefore, we apply the principles of traditional liposuction with those of subdermal superficial liposuction to aspirate large amounts of fat from all the adipose layers. We call this technique Massive All Layer Liposuction (MALL). The amount of skin shrinkage after this "defatting" procedure is remarkable and the clinical results are very good. The MALL technique can be applied to other areas as well. In our experience this new liposuction technique has dramatically reduced the indications of abdominoplasties and dermolipectomies of inner thighs and arms. 相似文献