Structural and pharmacological characterization of phenylalanine-based AMPA receptor antagonists at kainate receptors

Continued efforts into the discovery of ligands that target ionotropic glutamate receptors (iGluRs) are important for studies of the physiological roles of the various iGluR subtypes as well as for the search for drugs that can be used in the treatment of diseases of the central nervous system. A new series of phenylalanine derivatives that target iGluRs was reported to bind AMPA receptors. Herein we report our studies of these compounds at the kainate receptors GluK1-3. Several compounds bind with micromolar affinity at GluK1 and GluK3, but do not bind GluK2. The crystal structure of the most potent compound in the ligand binding domain of GluK1 revealed different modes of binding to GluK1 and GluA2, due primarily to residues Ser741 (GluK1) and Met729 (GluA2). The compound was shown to be slightly more potent at GluK1 than at AMPA receptors and to induce a domain closure similar to that observed in GluK1 structures with partial agonists.     

Field Investigation of a Sandwich Plate System Bridge Deck

This paper presents the results of a live-load test of the Shenley Bridge, the first bridge application of the sandwich plate system technology in North America. The investigation focused on the evaluation of in-service performance including lateral load distribution behavior and dynamic load allowance. Real-time midspan deflections and strain values were measured under both static and dynamic conditions and under various loading configurations to assess the in-service performance. Distribution factors were determined for interior and exterior girders subjected to single and paired truck loadings. In addition, dynamic load allowance was determined from a comparison of the bridge’s response under static conditions to the response under dynamic conditions. From a comparison of measured results to AASHTO LRFD, AASHTO standard, and CHBDC provisions, it was determined that the current provisions tend to produce conservative predictions for lateral load distribution, but can be unconservative for dynamic load allowance. As a result of the testing program containing a single field test, a finite-element model was also used for determination of lateral load distribution and yielded predictions similar to measured results. The results from the finite-element models were often less conservative than the code provisions.     

铝电解中二氧化锡基（氢气）气体阳极

开发一种在低温电解质（850℃）铝电解中应用的新型二氧化锡基气体阳极以降低铝电解能耗和二氧化碳排放。在此种气体阳极中,氢气通入阳极后参与阳极反应,分别用石墨和铝作阴极和参比电极。采用循环伏安法研究此体系中铂和二氧化锡基电极上氧离子的电化学行为,并确定氧气的析出电势。然后,采用气体电极进行恒电流电解实验,相对于未通入气体和通入氩气,通入氢气后阳极出现明显的去极化现象（阳极电势约下降0.8 V）。实验结果表明,氢气已参与阳极三相界面（气体-电解质-电极）反应,为还原性气体阳极在铝电解上的应用提供了可行性。     

Protocol for evaluating the efficacy of cetylpyridinium chloride as a beef hide intervention

The objective of this study was to establish the necessary protocols and assess the efficacy of cetylpyridinium chloride (CPC) as an antimicrobial intervention on beef cattle hides. Experiments using CPC were conducted to determine (i) the methods of neutralization needed to obtain valid efficacy measurements, (ii) the effect of concentration and dwell time after treatment, (iii) the effect of CPC on hide and carcass microbial populations when cattle were treated at a feedlot and then transported to a processing facility for harvest, and (iv) the effectiveness of spray pressure and two-spray combinations of CPC and water to reduce hide microbial populations. Residual CPC in hide sponge samples prevented bacterial growth. Dey-Engley neutralization media at 7.8% and a centrifugation step were necessary to overcome this problem. All dwell times, ranging from 30 s to 4 h, after 1% CPC application to cattle hides resulted in aerobic plate counts and Enterobacteriaceae counts 1.5 log CFU/100 cm2 lower than controls. The most effective dose of CPC was 1%, which reduced aerobic plate counts and Enterobacteriaceae counts 2 and 1 log CFU/100 cm2, respectively. Low-pressure application of 1% CPC at the feedlot, transport to the processing facility, and harvest within 5 h of application resulted in no effect on Escherichia coli O157 prevalence on hides or preevisceration carcasses. Two high-pressure CPC washes lowered aerobic plate counts and Enterobacteriaceae counts by 4 log CFU/100 cm2, and two medium-pressure CPC washes were only slightly less effective. These results indicate that under the proper conditions, CPC may still be effective for reducing microbial populations on cattle hides. Further study is warranted to determine if this effect will result in reduction of hide-to-carcass contamination during processing.     

Developing a Risk Assessment Model for construction safety

Problems on occupational health and safety are commonly occurred in the construction industry, including falling of materials or person from height, stepping on objects and injured by hand tools. Key of dealing with project safety and risk assessment in construction industry is important, thus, way on doing assessment and liability of assessment are necessary for all professionals. However, it is found that those professionals prone to heavily rely on their own experiences and knowledge on decision making on risk assessment, which lack of a systematic approach and lack of ways to check the reliability of the decisions. This paper investigates the need for people involved in construction to take systematic and effective risk assessments for different trades. 14 common types of trades, accidents, and accident causes are explored, in addition to suitable risk assessment methods and criteria. A Risk Assessment Model (RAM) is then developed for assessing risk levels as various project stages with various work trades. A case study is used to prove the workability and reliability of the developed RAM in the practical industry. The developed RAM is found to be benefit for predicting high-risk construction activities and thus preventing accidents occurred based on a set of historical accident data.     

TIME-CRITICAL COMMUNICATION AND COMPUTATION FOR INTELLIGENT VEHICULAR NETWORKS

Vehicular networks are expected to empower auto V mated driving and intelligent transportation via vehicle-to-e very thing(V2X)communications and edge/cloud-ass...