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51.
Abstract— A new light‐collection optics has been developed that enhances the luminance of projection TV which use lamps as the light source. The conventional optical system consists of an elliptical reflector and a flat‐surface front glass, but these systems cannot sufficiently collect the beams coming from the light source, and they cause loss in the coupling with the light pipe. To solve this problem, we devised a new optical system through a structure of an aspherical reflector and an aspherical front glass. This new optical system concentrates the beams coming from the light source to a smaller point which improves the coupling efficiency. Thus, we have successfully increased the luminance of the projection TV by approximately 10%. This paper reports the design principles of the new optical system and the results of a prototype experiment.  相似文献   
52.
The establishment and maintenance of axonal patterning is crucial for neuronal function. To identify the molecular systems that operate locally to control axonal structure, it is important to manipulate molecular functions in restricted subcellular areas for a long period of time. Microfluidic devices can be powerful tools for such purposes. In this study, we demonstrate the application of a microfluidic device to clarify the function of local Ca2+ signals in axons. Membrane depolarization significantly induced axonal branch-extension in cultured cerebellar granule neurons (CGNs). Local application of nifedipine using a polydimethylsiloxane (PDMS)-based microfluidic device demonstrated that Ca2+ entry from the axonal region via L-type voltage-dependent calcium channels (L-VDCC) is required for branch extension. Furthermore, we developed a method for locally controlling protein levels by combining genetic techniques and use of a microfluidic culture system. A vector for enhanced green fluorescent protein (EGFP) fused to a destabilizing domain derived from E. coli dihydrofolate reductase (ecDHFR) is introduced in neurons by electroporation. By local application of the DHFR ligand, trimethoprim (TMP) using a microfluidic device, we were able to manipulate differentially the level of fusion protein between axons and somatodendrites. The present study revealed the effectiveness of microfluidic devices to address fundamental biological issues at subcellular levels, and the possibility of their development in combination with molecular techniques.  相似文献   
53.
Research on noise robust speech recognition has mainly focused on dealing with relatively stationary noise that may differ from the noise conditions in most living environments. In this paper, we introduce a recognition system that can recognize speech in the presence of multiple rapidly time-varying noise sources as found in a typical family living room. To deal with such severe noise conditions, our recognition system exploits all available information about speech and noise; that is spatial (directional), spectral and temporal information. This is realized with a model-based speech enhancement pre-processor, which consists of two complementary elements, a multi-channel speech–noise separation method that exploits spatial and spectral information, followed by a single channel enhancement algorithm that uses the long-term temporal characteristics of speech obtained from clean speech examples. Moreover, to compensate for any mismatch that may remain between the enhanced speech and the acoustic model, our system employs an adaptation technique that combines conventional maximum likelihood linear regression with the dynamic adaptive compensation of the variance of the Gaussians of the acoustic model. Our proposed system approaches human performance levels by greatly improving the audible quality of speech and substantially improving the keyword recognition accuracy.  相似文献   
54.
Vitamin D3 hydroxylase (Vdh) from Pseudonocardia autotrophica is a cytochrome P450 monooxygenase that catalyzes the two‐step hydroxylation of vitamin D3 (VD3) to produce 25‐hydroxyvitamin D3 (25(OH)VD3) and 1α,25‐dihydroxyvitamin D3 (1α,25(OH)2VD3). These hydroxylated forms of VD3 are useful as pharmaceuticals for the treatment of conditions associated with VD3 deficiency and VD3 metabolic disorder. Herein, we describe the creation of a highly active T107A mutant of Vdh by engineering the putative ferredoxin‐binding site. Crystallographic and kinetic analyses indicate that the T107A mutation results in conformational change from an open to a closed state, thereby increasing the binding affinity with ferredoxin. We also report the efficient biocatalytic synthesis of 25(OH)VD3, a promising intermediate for the synthesis of various hydroxylated VD3 derivatives, by using nisin‐treated Rhodococcus erythropolis cells containing VdhT107A. The gene‐expression cassette encoding Bacillus megaterium glucose dehydrogenase‐IV was inserted into the R. erythropolis chromosome and expressed to avoid exhaustion of NADH in a cytoplasm during bioconversion. As a result, approximately 573 μg mL?1 25(OH)VD3 was successfully produced by a 2 h bioconversion.  相似文献   
55.
56.
A fabrication technique of high-purity vertically aligned single-walled carbon nanotubes (VA-SWCNTs) using atmospheric pressure plasma enhanced chemical vapor deposition is presented. Although densely mono-dispersed Fe-Co catalysts of a few nanometers is primarily responsible for VA-SWCNT growth, carbon precipitation was virtually absent in the thermal CVD regime at 700 °C. On the other hand, high-purity VA-SWCNTs without measurable defects were grown at 4 μm min−1 by applying atmospheric pressure radio-frequency discharge (APRFD) which has been previously developed for this purpose. The results proved that cathodic ion sheath adjacent to the substrates, where a large potential drop exists, also plays an essential role for the controlled growth of SWCNTs, while ion damage to the VA-SWCNTs is inherently avoided due to high collision frequency among molecules in atmospheric pressure. Operation regime of APRFD and tentative reaction mechanisms for VA-SWCNT growth are discussed along with optical emission spectroscopy of near substrate region.  相似文献   
57.
Abatement of CO2 emission is one of the most important issues in the 21st century regarding preservation of the earth environment. This paper addresses a utility operations planning problem for distributed energy management systems (DEMSs), where we are to obtain optimal plans that minimize both costs and CO2 emissions. A DEMS consists of multiple entities that seek their own economic profits. In this paper, we give a mathematical formulation of the utility operations planning problem for each entity, and propose an energy trading market, which utlizes a multi‐attribute auction protocol in order to deal with both a price and a CO2 emission rate. Experimental results show that collaboration among entities through the market provides a more profitable plan for each entity and abatement of CO2 emission is also achieved. © 2007 Wiley Periodicals, Inc. Electr Eng Jpn, 162(4): 54–63, 2008; Published online in Wiley InterScience ( www.interscience.wiley. com ). DOI 10.1002/eej.20418  相似文献   
58.
For the purpose of developing liquid crystalline microactuators, the transient behaviors of a nematic liquid crystal between two parallel plates have been computed for various parameters such as applied voltage, the gap between the plates, and the twist and tilt angles at the plates. The Leslie-Ericksen theory has been selected as a constitutive equation. The twist angle has an effect on the induced velocity profiles; for example, the induced flow is planar at the twist angle of 0°, while the flow has an out-of-plane component when the twist angle is not 0°. Transient behaviors of shear stress acting on the plates, the flowrate, and the maximum values of the velocity, and the tilt angle between the plates have been reported. In addition, we have investigated the effects of the applied voltage, the gap between the plates, and the tilt angle at the plates on the above-mentioned values. We can develop microactuators with arbitrary characteristics by suitably controlling the applied voltage, the size of the actuators, and the director anchoring conditions.  相似文献   
59.
We have attempted to develop high‐performance and safe fuel cells by using ammonium formate as a solid (powder) fuel. This solid fuel has the potential of safer transportability than liquid fuels such as methanol from the viewpoint of toxicity and flammability. In order to make use of some of the advantages of ammonium formate, we investigated the oxidation characteristics of ammonium formate with respect to a Pt electrode. Cyclic voltammograms indicate that ammonium formate has high oxidation activity with respect to a Pt electrode. We have also found that the oxidation of ammonium formate can be improved by the addition of Ir to Pt catalysts. It is highly likely that ammonium formate will be useful as a solid (powder) fuel for polymer electrolyte membrane (PEM) fuel cells. This new fuel will promote the development of safe fuel cells for PEM. © 2010 Wiley Periodicals, Inc. Electr Eng Jpn, 174(4): 45–50, 2011; Published online in Wiley Online Library ( wileyonlinelibrary.com ). DOI 10.1002/eej.21043  相似文献   
60.
A method to construct optimal memoryless regulators of large‐scale systems with time delays in the interconnections and the states is proposed. Each feedback gain of decentralized control laws is obtained directly from solutions of simultaneous linear matrix inequalities. First, a sufficient condition for the stability of the overall closed‐ loop system is presented. Then it is extended so that the overall system and each subsystem remain stable, even if all or some of the interconnections between subsystems are cut. Lastly, a simplification of the implementation is discussed in the perspective of feedback loop reduction. © 2011 Wiley Periodicals, Inc. Electr Eng Jpn, 177(4): 42–50, 2011; Published online in Wiley Online Library ( wileyonlinelibrary.com ). DOI 10.1002/eej.21191  相似文献   
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