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91.
M Minami S Kimura T Endo N Hamaue M Hirafuji H Togashi M Matsumoto M Yoshioka H Saito S Watanabe T Kobayashi H Okuyama 《Canadian Metallurgical Quarterly》1997,58(4):1123-1129
We have recently shown that inferior performance in passive avoidance task is accompanied with decreased hippocampal choline (Ch) in stroke-prone spontaneously hypertensive rats (SHRSP) compared with normotensive control Wistar-Kyoto rats (WKY). We also reported that dietary docosahexaenoic acid (DHA) suppresses the development of hypertension and stroke-related behavioral changes, resulting in the prolongation of the life span of SHRSP. In this study, we examined the effect of dietary DHA on the cerebral acetylcholine (ACh) levels and learning performance in passive avoidance tasks in SHRSP. The arachidonic acid decreased and the DHA increased in plasma lipids dose dependently with dietary DHA treatments, which decreased the systolic blood pressure in SHRSP. Dietary DHA significantly restored the significantly inferior learning performance in passive avoidance response observed in control SHRSP (DHA 0%). Furthermore, the hippocampal ACh levels were correlated positively with the total response latency in passive avoidance tasks. These results suggest that cholinergic dysfunction in the brain of control SHRSP is responsible, at least in part, for the impaired learning ability and the dietary DHA ameliorates this performance failure. 相似文献
92.
H Itatani H Itoh T Yoshioka M Namiki T Koide A Okuyama T Sonoda 《Canadian Metallurgical Quarterly》1981,19(2):119-122
In histochemical studies it was shown that sulfated acid glycosaminoglycans (AGAGS) were produced and secreted into the tubular lumen in renal papilla, but not in the renal cortex of muddy stone forming kidney. There was no secretion of sulfated AGAGS in renal papilla histochemically during hydronephrosis before stone formation. On autoradiographic study with the use of 45Ca and 35S for labeling of sulfated AGAGS, we found that 45Ca accumulated in renal papilla of muddy stone forming kidney, but not in the other. 35S apparently accumulated into muddy stones. Measurement of calcium content of the renal papilla and cortex proved the results of autoradiographic studies, and measurement of uronic acid in the urine showed increased secretion of AGAGS in the urine from muddy stone forming kidney. From these results it was proposed that the sulfate AGAGS secreted in the urine could bind calcium crystals to each other amd make crystals aggregate massively. 相似文献
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94.
Y Okuyama M Yamada C Kondo E Satoh S Isomoto T Shindo Y Horio M Kitakaze M Hori Y Kurachi 《Canadian Metallurgical Quarterly》1998,435(5):595-603
The aim of this study was to evaluate whether long-term administration of L-arginine, a physiological substrate for the production of nitric oxide, improved blood pressure, heart rate, cardiac hypertrophy and particularly structural changes in the coronary and carotid artery of spontaneously hypertensive rats (SHR). The experiments started with three groups of 10-week-old animals: control Wistar rats, untreated SHR and SHR treated with L-arginine (SHR + L-arginine). L-Arginine was administered to SHR in a daily dose of 300 mg kg-1 intraperitoneally for 6 weeks. Blood pressure and heart rate were recorded each week. At the end of the experiment in one-half of each group heart weight and body weight were determined and the heart weight/body weight index was calculated. In the other animals, the cardiovascular system was perfused via the left ventricle with a glutaraldehyde fixative at 120 mmHg and the coronary and carotid arteries were processed for transmission electron microscopy. The inner diameter and wall thickness (tunica intima and tunica media) were measured on semithin sections. The reliability of the genetic feature in the SHR group was proved by the increased heart weight, heart weight/body weight index, wall thickness and wall thickness/inner diameter ratio of coronary and carotid arteries in comparison to the group of control Wistar rats. Long-term administration of L-arginine did not significantly influence blood pressure and heart rate in comparison with untreated SHR. Neither were any differences found in cardiac hypertrophy or the geometry of the coronary and carotid arteries (thickness of arterial wall, inner diameter, wall/diameter ratio). In conclusion, the changes in the cardiovascular system in SHR were not reversed, or even alleviated, by chronic treatment with L-arginine. 相似文献
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The applicability of a powerful but still easy to use technique, based on a phenomenological theory of viscoelasticity, for processing and analyzing dynamic mechanical data of some rice gels was investigated. Based on this theory a continuous relaxation spectra was generated by application of Tikhonov regularization procedure on continuous Maxwell model. Interpretation of relaxation spectra in terms of number of peaks, its peak intensity H(λ) and appearance of its main distribution peak and magnitude of equilibrium elasticity modulus (Ge) of continuous Maxwell model was found to appropriately reflect main peculiarities of the viscoelastic behavior of rice starch gels. An increase in number of peaks in the relaxation spectra was observed for starch gels having higher amylose content indicating the creation of more heterogeneous structure. H(λ) and Ge values also increased with increase in amylose content demonstrating a transition of the system to more stable state like a gel. 相似文献
98.
In the straightness profile measurement of a mechanical workpiece, hardware datums have been the traditional standard. However, error separation techniques of the surface profile from parasitic motions have been developed. These are known as software datums, which separate the surface profile from the parasitic motions using multiple sensors and/or multiple orientations and realize higher accuracy than that of the hardware datum. However, the conventional software datum cannot measure a large-scale workpiece because the large sampling number causes random error amplification. Furthermore, the conventional software datum assumes that sensor's random noise is small enough in comparison with the parasitic motions. But, the accuracy of the hardware datum has become high. Then, the accuracy of the sensor's random noise is not so small, relatively. In this paper, a next-generation software datum, the two-point method based on the least uncertainty propagation, is proposed. The proposed two-point method consists of weighting and inverse filtering, resulting in the least uncertainty of the estimated surface profile by choosing suitable weighting. 相似文献
99.
Komada S. Hashimoto Y. Okuyama N. Hisada T. Hirai J. 《Industrial Electronics, IEEE Transactions on》2009,56(10):3914-3920
Although much equipment for physical therapy has been developed, equipment to improve the quality of physical therapy is scarce. We propose a robotic biofeedback exercise device that can display human joint torque and muscle force during training without a problematic electromyogram (EMG). The purpose is to increase the therapeutic value by understanding a person's condition during exercise and to provide an incentive to improve performance. The manipulator supports lower limb rehabilitation in the sagittal plane. With its ability to adjust the maximum speed and the time constant, the manipulator provides simultaneous and safe isokinetic exercise for the knee and hip joints. This paper describes the estimation of the human joint torque and muscle force. The display of the joint torque and the muscle force is realized during exercise of the knee joint using the developed manipulator. The estimation of the muscle force from Crowninshield's method and Hase's method generally agrees with the EMG. 相似文献
100.
Yusuke Sakai Tomoko TanakaJunji Fukuda Kohji Nakazawa 《Journal of Bioscience and Bioengineering》2010,109(4):395-399
Hepatocyte multicellular aggregates (spheroids), which maintain high expression of liver functions, have been advocated as a useful culture technique for various cell-based assays. In this study, we investigated the drug metabolic function of a hepatocyte spheroid microarray (HSM) chip, which contained an array of 672 spheroids of primary rat hepatocytes within a 100-mm2 region in the center of a poly(methylmethacrylate) plate (24 × 24 mm) and used an alkoxyresorufin (ethoxy-, methoxy-, pentoxy- and benzyloxyresorufin) O-dealkylase assay system. Ethoxyresorufin O-dealkylase (EROD) activity of the HSM chip initiated by 3-methylcholanthrene (3-MC), an inducer of cytochrome P450 enzymes, was 5- to 10-fold higher than that of monolayer hepatocytes, with activity being maintained for at least 2 weeks. We also demonstrated that 3-MC induced EROD, methoxyresorufin O-dealkylase (MROD) and benzyloxyresorufin O-dealkylase (BROD) activities in the HSM chip, while sodium phenobarbital (P450 inducer) induced pentoxyresorufin O-dealkylase (PROD), BROD, EROD and MROD activities. Induction of these activities was confirmed by increased gene expression of the related P450 enzymes. These results showed that the HSM chip had a good response to P450 inducers and that function was maintained for long periods of time. The HSM chip therefore may be a promising cellular platform for drug metabolic assays using hepatocytes. 相似文献