Assimilative and co-metabolic degradation of chloral hydrate by bacteria and their bioremediation potential

Although the bacterial degradation of chloral hydrate (CH) has been recognized for several decades, its degradation pathway by assimilation has not been demonstrated. In this paper, we report the isolation of the LF54 bacterial strain, which utilizes CH as its sole carbon and energy source. LF54 converted CH into trichloroethanol (TCAol), which was dehalogenated to dichloroethanol (DCAol), and CO(2) was detected as the end product. Another strain that we isolated, RS20, co-metabolized CH into TCAol. Our 16S rRNA gene sequencing and taxonomic analyses revealed that the LF54 and RS20 strains belong to the Pseudomonas and Arthrobacter genera, respectively. When the two strains were inoculated into soil microcosms, both degraded 0.3mM CH to undetectable levels (<0.01mM) within 5days. These results suggest that LF54 and RS20 could be used in the bioremediation of CH-contaminated environments.     

Environmental ecological modeling of human blood lead levels in East Asia

Environmental ecological modeling (EEM), which unifies models simulating transport of chemicals and exposure of humans to chemicals, was used to simulate long-term trends of female adult human blood lead levels (BLLs) and historical exposure to the atmospheric lead in four East Asian countries: Japan, Korea, China, and Vietnam. Anthropogenic lead emissions to the atmosphere in Vietnam were estimated from energy statistics to be 1931 t yr(-1). Calculated BLLs generally agreed with those observed in samples collected in these countries as the error factors were less than 2. The model results revealed that BLLs decreased significantly in Tokyo (by 58%) and Seoul (by 45%) in recent decades and confirmed the effects of efforts to reduce environmental lead in Japan and Korea. The model results also revealed that BLLs in Beijing did not decrease in this decade as much as in Tokyo and Seoul, despite the phasing out of leaded gasoline, and that the contribution from the atmospheric component was increasing (43% in 2009). Finally, we applied EEM to simulate BLLs of children in Hanoi. The probability of children having BLLs greater than 50 μg L(-1) was 7.5%, which was greater than those observed in developed countries.     

Study on the compressible viscous flows through a straight pipe

When sonic nozzles of significantly smaller diameter are used as standard flow meters, the critical backpressure ratio is affected by the boundary layer at the nozzle throat. It is known that, as the flow pathway is complicated in actual valves, the critical pressure ratio differs from the ideal value. However, the effect of the boundary layer thickness on choking criteria is still controversial. Choking phenomenon in a sonic nozzle fitted with a straight circular pipe of variable length is investigated numerically and the results are compared with experimental and theoretical ones. The results show that the actual critical pressure ratio is less than the ideal value and it decreases with increase in pipe length. The effect of boundary layer thickness on main flow Mach number is also made clear in this work. In addition, sonic conductance and pressure loss in actual viscous flows computed are compared with the ISO 6358 standard.     

Experimental study on hysteresis phenomena of shock wave structure in an over-expanded axisymmetric jet

In recent studies on two-dimensional supersonic jets, it is reported that the hysteresis phenomenon for the reflection type of shock waves in the jet flow field is occurred under the quasi-steady flow condition and this phenomenon is affected by the transitional pressure ratio between the regular reflection and Mach reflection. However, so far, there are few researches on the hysteresis phenomenon for the transition of shock waves between regular and Mach reflection in over-expanded supersonic jets and the phenomenon has not been investigated satisfactorily. Therefore, the purpose of this study is to clarify the hysteresis phenomena for the reflection type of shock wave in the over-expanded axi-symmetric supersonic jet experimentally, and to discuss the relationship between hysteresis phenomenon and rate of the change of pressure ratio with time. Furthermore, the effect of Mach number at the nozzle exit on hysteresis loop was investigated for two kinds of nozzle.     

Development of 72‐kV high‐pressure air‐insulated GIS with vacuum circuit breaker

SF₆ gas has excellent dielectric strength and interruption performance. For these reasons, it has been widely used for gas‐insulated switchgear (GIS). However, use of SF₆ gas has become regulated under agreements set at the 1997 COP3. Thus, investigation and development for GIS with a lower amount of SF₆ gas are being carried out worldwide. Presently, SF₆‐free GIS has been commercialized for the 24‐kV class. Air or N₂ gas is used as the insulation gas for this GIS. On the other hand, SF₆‐free GIS has not been commercialized for the 72‐kV‐class GIS. The dielectric strengths of air and N₂ gas are approximately one‐third that of SF₆ gas. To enhance the insulation performance of air and N₂ we have investigated a hybrid gas insulation system which has the combined features of providing an insulation coating and suitable insulation gas. We have developed the world's first 72‐kV SF₆‐free GIS. This paper deals with key technologies for SF₆‐free GIS, such as the hybrid insulation structure, a bellows for the high‐pressure vacuum circuit breaker, a newly designed disconnector and spacer, and prevention of particle levitation. Test results of the 72‐kV high‐pressure air‐insulated GIS with the vacuum circuit breaker are described. © 2006 Wiley Periodicals, Inc. Electr Eng Jpn, 157(4): 13–23, 2006; Published online in Wiley InterScience ( www.interscience.wiley.com ). DOI 10.1002/eej.20451     

Thermal link for cartridge-type cryostat

We have developed a simple and small thermal link for cooling a cylindrical cartridge, on which device under test is mounted. It consists of a crown-like ring with an inner diameter of 170 or 140 mm and a clamping belt, which is a metal spring or nylon. A thermal conduction of the thermal link is achieved as the crown-like ring clamps the disk-like stage of the cartridge with external force of the clamping belt. This link can be applied at various temperature ranges from 2 to 100 K. The measured thermal conductance of the 170 mm link is 1.7, 5.6 and 3.3 W K⁻¹ for 4, 12, and 80 K stages, respectively. These values are consistent with a empirical calculation within 10% errors. This link is also effective to reduce mechanical vibration to be 6 μm (peak-to-peak) in the horizontal direction of the cartridge. This link is easily fabricated and is useful to various detectors which require to be cooled down.     

Evaluation of serum KL-6 levels in summer-type hypersensitivity pneumonitis

A high level of serum KL-6 is a known feature of active pulmonary fibrosis. Some researchers have suggested that KL-6 is produced and secreted by type II pneumocytes. The present study evaluated serum KL-6 levels in patients with summer-type hypersensitivity pneumonitis (summer-type HP) (n = 6, 7 episodes), Mycoplasma pneumoniae pneumonia (n = 16), Chlamydia psittaci pneumonia (n = 3), Chlamydia pneumoniae pneumonia (n = 9), and bacterial pneumonia (n = 12). In addition, transbronchial lung biopsy (TBLB) specimens were examined pathologically in order to identify the site of production and secretion of KL-6. In patients with summer-type HP, the serum KL-6 levels exceeded 500 U/ml (2.996 +/- 2.016 U/ml), but was below 500 U/ml (302 +/- 126 U/ml, p < 0.001) in the patients with other infectious pneumonias, with the exception of two. One of these two patients with a high serum KL-6 level had adult respiratory distress syndrome due to Mycoplasma pneumoniae. The other had organizing pneumonia due to Chlamydia pneumoniae. TBLB specimens showed proliferative type II pneumocytes in all summer-type HP cases. We believe that the high serum KL-6 levels were produced by type II pneumocytes, and may provide a useful indicating serum marker for HP. Although serum LDH, serum CRP and PaO2 are known as monitoring markers in summer-type HP, our findings demonstrated no manifest correlations among these markers. However, serum KL-6 levels showed a strong positive correlation with serum LDH levels and an inverse correlation with serum CRP levels. These results suggest that serum KL-6 may be a better marker of the degree of disease activity than serum LDH, CRP, or PaO2 in summer-type HP.     

Effects of Hub-To-Tip Ratio and Tip Clearance on Hysteretic Characteristics of Wells Turbine for Wave Power Conversion

A Wells turbine for wave power conversion has hysteretic characteristics in a reciprocating flow. The hysteretic loop is opposite to the well-known dynamic stall of an airfoil. In this paper, the mechanism of the hysteretic behavior was elucidated by an unsteady 3-dimensional Navier-Stokes numerical simulation. It was found that the hysteretic behavior was associated with a streamwise vortical flow appearing near the blade suction surface. The effects of hub-to-tip ratio and tip clearance on the hysteretic characteristics of the Wells turbine have also been discussed in this paper.     

Influence of Carbon Forms and Doped Elements in the Electrodes for the Production of Fullerenes

Production of fullereries using electrodes made of four kinds of pure carbon bearing materials and artificial graphite doped with seven kinds of elements such as B, Y, La, Gd, Ce, Pr and Nd have been examined. The yields of fullerenes were in a range between 12-20% of the soot produced for all pure carbon electrodes, even though the vaporization rate of amorphous carbon electrodes was about ten times higher than that of the natural graphite electrodes. However, for the doped graphite electrodes, the yield of fullerenes was less than 5%. From the above results it is concluded that fullerenes can be effectively produced with the pure carbon electrodes made of any form of carbon bearing materials used in this study and there is little material dependence on the yield of fullerences. Furthermore, the experiments using the artificaial graphite electrodes doped with metal elements suggests that the purity of the carbon electrodes affects the yeld of fullerenes greatly.     

Catalytic synthesis of N-alkylacrylamide from acrylonitrile and alcohol with solid acids

Catalytic synthesis of N-alkylacrylamides, which are useful monomers for functional polymers, from acrylonitrile and alcohols, such as 1-adamantanol, tert-butyl alcohol, and isopropyl alcohol has been studied using various solid acids including heteropoly compounds and zeolites. For the reaction with 1-adamantanol or tert-butyl alcohol, a heteropoly compound, Cs_2.5H_0.5PW₁₂O₄₀, exhibited a high catalytic performance and water-tolerance. On the other hand, for the synthesis of N-isopropylacrylamide from isopropyl alcohol, a high-silica zeolite, H-ZSM-5 with Si/Al ratio of 37, showed an exceptionally high activity, while it was less active in the reaction with tert-butyl alcohol or 1-adamantanol. This marked effect of reactant alcohol observed for H-ZSM-5 is probably due to shape selectivity. The prominent performance for N-isopropylacrylamide synthesis is attributable to a steric effect of the constrained pores; two reactants might be arranged adequately to form an intermediate in the pores.