Highly unsaturated fatty acid might act as an antioxidant in emulsion system oxidized by azo compound

Now it is recognized that DHA is oxidatively stable fatty acid compared with linoleic acid (LA) in emulsified system, although DHA is oxidatively unstable in a bulk system. In fact, an emulsified mixture of DHA and LA behaves as in a bulk system, namely the oxidative stability of DHA becomes lower than that of LA. Therefore, in this study, tridocosahexaenoate (DDD) and glycerol trilinoleate (LLL) were separately emulsified using TritonX-100 as an emulsifier and DDD emulsion was mixed with the oxidizing LLL emulsion using a water-soluble radical initiator, 2,2'-azobis(2-aminopropane) dihydrochloride. As a result, DHA suppressed the oxidation of LA, while DHA was not significantly oxidized. This suppression ability was examined using glycerol trieicosapentaenoate, glycerol trilinolenate, or glycerol trioleate instead of DDD and it was found that this activity was increased with the increasing number of double bonds in the structure. Furthermore, the same type of experiment was carried out using a lipid-soluble radical initiator, 2,2'-azobisisobutyronitrile and the similar result was obtained. These results indicated that a highly polyunsaturated fatty acid might act as an antioxidant in an emulsion system oxidized by an azo compound.     

Simultaneous determination of five antioxidants in food by HPLC with fluorescence detection

An HPLC method with fluorescence detection was developed for the determination of propyl gallate, nordihydroguaiaretic acid, butylated hydroxyanisole (2- and 3-tert-butyl-4-hydroxyanisole), tert-butylhydroquinone and octyl gallate in edible oils and foods. The antioxidants in edible oil were isolated directly with acetonitrile saturated with n-hexane. The antioxidants in food were extracted with ethyl acetate and the extract was concentrated under vacuum. They were isolated from the residue with acetonitrile saturated with n-hexane. The acetonitrile layer was centrifuged at 5,000 rpm for 10 min. The HPLC separation was performed on a Symmetry C18 column (3.5 microns, 4.6 mm i.d. x 150 mm) using a mixture of 5% acetic acid-acetonitrile-methanol (4:3:3, v/v/v) as the mobile phase and monitored by using a fluorescence detector with time programming. Sample peaks were identified by comparison of the fluorescence spectra with those of antioxidant standards. Average recoveries of fortified antioxidants at 100 micrograms/g were 72.1-99.6%. Coefficients of variation were 0.7-7.2%.     

Current distribution calculation for a superconducting multi-layered power cable

The calculation method for the current distributions within a coaxial multi-layered power cable consisting of helically wound long superconducting tape conductors have been studied. The analytical expressions for the self- and mutual inductances of the coaxial helical long thin arch-shaped tapes are analytically and numerically compared with straight arch-shaped and flat tapes. It is confirmed that these expressions are consistent with each other. The current distributions within a coaxial 3-layered cable are calculated, using the self- and mutual inductances obtained due to the thin approximation. The effect of the non-uniform gaps between tapes in a coaxial multi-layered power cable is studied.     

Solid and thermal properties of ABA‐type triblock copolymers designed using difunctional fluorine‐containing polyimide macroinitiators with methyl methacrylate

BACKGROUND: ABA‐type poly(methyl methacrylate) (PMMA) and fluorine‐containing polyimide triblock copolymers are potentially beneficial for electric materials. In the work reported here, triblock copolymers with various block lengths were prepared from fluorine‐containing difunctional polyimide macroinitiators and methyl methacrylate monomer through atom‐transfer radical polymerization. The effects of structure on their solid and thermal properties were studied. RESULTS: The weight ratios of the triblock copolymers derived using thermogravimetric analysis were shown to be almost identical to the ratios determined using ¹H NMR. The solid properties (film density and maximum d‐spacing value) and thermal properties (glass transition and thermal expansion) were shown to be strongly dependent on the weight ratios of both PMMA and polyimide components. Furthermore, a porous film, which showed a lower dielectric constant of 2.48 at 1 MHz, could be prepared by heating a triblock copolymer film to induce the thermal degradation of the PMMA component. CONCLUSION: The use of the polyimide macroinitiator was useful in the preparation of ABA‐type triblock copolymers to control each block length that influences the solid and thermal properties. Additionally, the triblock copolymers have great potential in preparing porous polyimides in the application of electric materials as interlayer insulation membranes of large‐scale integration. Copyright © 2009 Society of Chemical Industry     

Microstructural refinement of an As-cast Al-12.6 wt pct Si alloy by repeated thermomechanical treatment to produce a heavily deformable material

A certain degree of cold working is advantageous in developing a fine microstructure with minute silicon crystals for eutectic and/or hypereutectic Al-Si cast alloys. A novel process, repeated thermomechanical treatment (RTMT), was applied to an Al-12.6 wt pct Si cast alloy. The process involves multiple-pass cold working (less than a 20 pct reduction in section area) and heat treatment at 793 K for 3.6 ks. Cold-work annealing was repeated up to about an 80 pct reduction in section from the beginning. The RTMT material showed a refined microstructure with high ductility. Most silicon crystals were fragmented to only a few micrometers and were spheroidized. The RTMT material showed such marked plasticity that it could be wrought up to a 99 pct reduction in section at room temperature. The Cold-worked RTMT materials exhibited an excellent balance between tensile strength and elongation and a higher strain hardening than the cast material.     

Purification and cloning of a thermostable manganese catalase from a thermophilic bacterium

We have purified a heat-stable catalase from a thermophilic bacterium, Thermus species strain YS 8-13. The enzyme was purified 160-fold from crude cellular extracts and possessed a specific activity of 8000 units/mg at 65 degrees C. The purified enzyme displayed the highest activity at pH 7 to 10 and temperatures around 85 degrees C. The catalase was determined to be a manganese catalase, based on results from atomic absorption spectra and inhibition experiments using sodium azide. The enzyme was composed of six identical subunits of molecular weight 36,000. Amino acid sequences determined from the purified protein were used to design oligonucleotide primers, which were in turn used to clone the coding gene. The nucleotide sequence of a 1.4-kb fragment of Thermus sp. YS 8-13 genomic DNA containing a 909-bp open reading frame was determined. The gene encoded a 302-residue polypeptide of deduced molecular weight 33,303. The deduced amino acid sequence displayed a region-specific homology with the sequences of the manganese catalase from a mesophilic organism, Lactobacillus plantarum.     

Carbon Dioxide Sensor Mechanism of Porous Hydroxyapatite Ceramics

Hydroxyapatite and Cl⁻ -containing hydroxyapatite powders are prepared and characterized. Reversible substitution of CO^2-₃ for OH⁻ at the surface is presumed to be responsible for the sensor function. The role of Cl⁻, which is necessary to realize the sensor function and is incorporated during soaking treatment, is considered as follows. It may reduce the strain caused by the incorporation of CO^2-₃ (which is larger than OH⁻), and, hence, promote the reversible substitution reaction. This behavior is presumed because Cl⁻ -containing hydroxyapatite samples exhibit sensor characteristics typical of CO₂ without any treatment.     

Surgical management of late esophageal perforation

A simple and rapid high-performance capillary electrophoresis (HPCE) method for the determination of 2-mercaptopyridine-1-oxide (pyrithione) was developed. After addition of ethylenediaminetetraacetic acid (EDTA), pyrithione was determined in the form of the free anion using 50 mM borate (pH 9.2) as background electrolyte and was detected at 244 nm with a limit of detection (LOD) of 0.636 ppm (S/N = 3). The method was used to check the purity of pyrithione preparations and for the determination of pyrithione in shampoo.     

Immunochemical approaches to AGE-structures: characterization of anti-AGE antibodies

An extractionless method for determining aflatoxin M1 (AFM1), a major metabolite of aflatoxin B1 (AFB1), in human urine was developed. The biological fluid is injected directly into the chromatographic system after simple dilution and centrifugation. A pre-column, packed with a cation-exchange phase and coupled on-line to a column-switching liquid chromatography (LC) system, is used for sample pre-treatment and concentration. The analytes are non-selectively desorbed with the LC eluent and cleaned by means of a column-switching procedure. Pre-treatment and analysis were performed within 40 min. Average AFMI recovery reached 97% in the 10-100 ng/l range of urine. The detection limit of AFM1 in urine and milk was 2.5 ng/l for 1 ml of injected sample. A comparison with an immunoaffinity column clean-up and LC method was performed. The method was applied to determine AFM1 in the urine of AFB1 gavaged rats, and in the urine of both potentially exposed and supposedly unexposed workers. The method was also extended to milk.