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排序方式： 共有645条查询结果，搜索用时 15 毫秒
611.
Brendon Wolff-Piggott Jesse Coleman Ulrike Rivett 《Information Technology for Development》2018,24(3):532-553
ABSTRACTThis exploratory investigation presents a case study of the deployment of an mHealth service in established public clinics, and assesses the findings using Activity Theory. We contribute to the limited empirical research on mHealth implementation in established public facilities, and build on work on the use of Activity Theory to frame investigations of ICT4D interventions.The study investigates the perspective of clinic staff responsible for registering women for a free maternal health messaging service. Open-ended interviews and observation sessions were used to reveal staff experiences of the implementation, and their work practices. Activity Theory analysis was adopted to help interpret the data, and identify likely dynamics leading to these specific practices.Some themes that emerged were the hierarchical nature of the medical profession and implications for task shifting, the influence of technical design choices on use patterns and issues arising from the developing-country context. 相似文献
612.
Research on dynamic and stochastic vehicle routing problems received increasing interest in the last decade. It considers a novel problem class, aiming at an appropriate handling of dynamic events combined with the incorporation of stochastic information about possible future events. This survey summarises the recent literature in this area. Besides, the classification according to the available stochastic information, a new classification based on the point in time where substantial computational effort for determining decisions or decision policies arises, is introduced. Furthermore, the difference in solution quality is analysed between approaches which consider either purely dynamic or stochastic problems compared to those which consider both, stochastic and dynamic aspects. A graphical representation demonstrates the strength of the reviewed approaches incorporating dynamic and stochastic information. The survey also gives an overview on the intensity of research for the different problem classes and its benefit in recent years. Finally, guidelines and promising directions for further research are presented. 相似文献
613.
Peter Gehrke Philip Hartjen Ralf Smeets Martin Gosau Ulrike Peters Thomas Beikler Carsten Fischer Carolin Stolzer Jürgen Geis-Gerstorfer Paul Weigl Sogand Schfer 《International journal of molecular sciences》2021,22(2)
Encouraging clinical results were reported on a novel cone-in-cone coupling for the fixation of dental implant-supported crowns (Acuris, Dentsply Sirona Implants, Mölndal, Sweden). However, the presence or absence of a microgap and a potential bacterial leakage at the conometric joint has not yet been investigated. A misfit and a resulting gap between the conometric components could potentially serve as a bacterial reservoir that promotes plaque formation, which in turn may lead to inflammation of the peri-implant tissues. Thus, a two-fold study set-up was designed in order to evaluate the bidirectional translocation of bacteria along conometrically seated single crowns. On conometric abutments filled with a culture suspension of anaerobic bacteria, the corresponding titanium nitride-coated (TiN) caps were fixed by friction. Each system was sterilized and immersed in culture medium to provide an optimal environment for microbial growth. Positive and negative controls were prepared. Specimens were stored in an anaerobic workstation, and total and viable bacterial counts were determined. Every 48 h, samples were taken from the reaction tubes to inoculate blood agar plates and to isolate bacterial DNA for quantification using qrt-PCR. In addition, one Acuris test system was subjected to scanning electron microscopy (SEM) to evaluate the precision of fit of the conometric coupling and marginal crown opening. Throughout the observational period of one week, blood agar plates of the specimens showed no viable bacterial growth. qrt-PCR, likewise, yielded a result approaching zero with an amount of about 0.53 × 10−4 µg/mL DNA. While the luting gap/marginal opening between the TiN-cap and the ceramic crown was within the clinically acceptable range, the SEM analysis failed to identify a measurable microgap at the cone-in-cone junction. Within the limits of the in-vitro study it can be concluded that the Acuris conometric interface does not allow for bacterial translocation under non-dynamic loading conditions. 相似文献
614.
Katharina Scherschel Hanna Bruninger Andrea Mlders Nadine Erlenhardt Ehsan Amin Christiane Jungen Ulrike Pape Diana Lindner Dane M. Chetkovich Nikolaj Klcker Christian Meyer 《International journal of molecular sciences》2021,22(9)
The tetratricopeptide repeat-containing Rab8b-interacting protein (TRIP8b/PEX5R) is an interaction partner and auxiliary subunit of hyperpolarization-activated cyclic nucleotide-gated (HCN) channels, which are key for rhythm generation in the brain and in the heart. Since TRIP8b is expressed in central neurons but not in cardiomyocytes, the TRIP8b-HCN interaction has been studied intensely in the brain, but is deemed irrelevant in the cardiac conduction system. Still, to date, TRIP8b has not been studied in the intrinsic cardiac nervous system (ICNS), a neuronal network located within epicardial fat pads. In vitro electrophysiological studies revealed that TRIP8b-deficient mouse hearts exhibit increased atrial refractory and atrioventricular nodal refractory periods, compared to hearts of wild-type littermates. Meanwhile, heart rate, sino-nodal recovery time, and ventricular refractory period did not differ between genotypes. Trip8b mRNA was detected in the ICNS by quantitative polymerase chain reaction. RNAscope in situ hybridization confirmed Trip8b localization in neuronal somata and nerve fibers. Additionally, we found a very low amount of mRNAs in the sinus node and atrioventricular node, most likely attributable to the delicate fibers innervating the conduction system. In contrast, TRIP8b protein was not detectable. Our data suggest that TRIP8b in the ICNS may play a role in the modulation of atrial electrophysiology beyond HCN-mediated sino-nodal control of the heart. 相似文献
615.
Frederik Braun Andreas Hentschel Albert Sickmann Theodore Marteau Swantje Hertel Fabian Frster Holger Prokisch Matias Wagner Saskia Wortmann Adela Della Marina Heike Klbel Andreas Roos Ulrike Schara-Schmidt 《International journal of molecular sciences》2021,22(15)
Mutations in the SPATA5 gene are associated with epilepsy, hearing loss and mental retardation syndrome (EHLMRS). While SPATA5 is ubiquitously expressed and is attributed a role within mitochondrial morphogenesis during spermatogenesis, there is only limited knowledge about the associated muscular and molecular pathology. This study reports on a comprehensive workup of muscular pathology, including proteomic profiling and microscopic studies, performed on an 8-year-old girl with typical clinical presentation of EHLMRS, where exome analysis revealed two clinically relevant, compound-heterozygous variants in SPATA5. Proteomic profiling of a quadriceps biopsy showed the dysregulation of 82 proteins, out of which 15 were localized in the mitochondrion, while 19 were associated with diseases presenting with phenotypical overlap to EHLMRS. Histological staining of our patient’s muscle biopsy hints towards mitochondrial pathology, while the identification of dysregulated proteins attested to the vulnerability of the cell beyond the mitochondria. Through our study we provide insights into the molecular etiology of EHLMRS and provide further evidence for a muscle pathology associated with SPATA5 deficiency, including a pathological histochemical pattern accompanied by dysregulated protein expression. 相似文献
616.
Katrin Herken Martin Glauner Stefanie C. Robert Matthias Maas Sonja Zippel Ulrike Nowak-Gttl Barbara Zieger Judith Lahav Anke C. Fender Kerstin Jurk Beate E. Kehrel 《International journal of molecular sciences》2021,22(9)
Platelet function is developmentally regulated. Healthy neonates do not spontaneously bleed, but their platelets are hypo-reactive to several agonists. The mechanisms underlying immature platelet function in neonates are incompletely understood. This critical issue remains challenging for the establishment of age-specific reference ranges. In this study, we evaluated platelet reactivity of five pediatric age categories, ranging from healthy full-term neonates up to adolescents (11–18 years) in comparison to healthy adults (>18 years) by flow cytometry. We confirmed that platelet hypo-reactivity detected by fibrinogen binding, P-selectin, and CD63 surface expression was most pronounced in neonates compared to other pediatric age groups. However, maturation of platelet responsiveness varied with age, agonist, and activation marker. In contrast to TRAP and ADP, collagen-induced platelet activation was nearly absent in neonates. Granule secretion markedly remained impaired at least up to 10 years of age compared to adults. We show for the first time that neonatal platelets are deficient in thrombospondin-1, and exogenous platelet-derived thrombospondin-1 allows platelet responsiveness to collagen. Platelets from all pediatric age groups normally responded to the C-terminal thrombospondin-1 peptide RFYVVMWK. Thus, thrombospondin-1 deficiency of neonatal platelets might contribute to the relatively impaired response to collagen, and platelet-derived thrombospondin-1 may control distinct collagen-induced platelet responses. 相似文献
617.
Carolina Sgarbi Ismael Malbrn Luciana Saldúa Gladys Albina Lori Ulrike Lohwasser Mian Abdur Rehman Arif Andreas Brner Marcos Yanniccari Ana Maria Castro 《International journal of molecular sciences》2021,22(24)
Fusarium head blight (FHB) of wheat, caused by Fusarium graminearum (Schwabe), is a destructive disease worldwide, reducing wheat yield and quality. To accelerate the improvement of scab tolerance in wheat, we assessed the International Triticeae Mapping Initiative mapping population (ITMI/MP) for Type I and II resistance against a wide population of Argentinean isolates of F. graminearum. We discovered a total of 27 additive QTLs on ten different (2A, 2D, 3B, 3D, 4B, 4D, 5A, 5B, 5D and 6D) wheat chromosomes for Type I and Type II resistances explaining a maximum of 15.99% variation. Another four and two QTLs for thousand kernel weight in control and for Type II resistance, respectively, involved five different chromosomes (1B, 2D, 6A, 6D and 7D). Furthermore, three, three and five QTLs for kernel weight per spike in control, for Type I resistance and for Type II resistance, correspondingly, involved ten chromosomes (2A, 2D, 3B, 4A, 5A, 5B, 6B, 7A, 7B, 7D). We were also able to detect five and two epistasis pairs of QTLs for Type I and Type II resistance, respectively, in addition to additive QTLs that evidenced that FHB resistance in wheat is controlled by a complex network of additive and epistasis QTLs. 相似文献
618.
For the modeling of the mechanical behavior of a two phase alloy with the rule of mixture (RM), the flow stress of both phases is needed. In order to obtain these information for the α′‐martensite in high alloyed TRIP‐steels, compression tests at cryogenic temperatures were performed to create a fully deformation‐induced martensitic microstructure. This martensitic material condition was subsequently tested under compressive loading at ?60, 20, and 100°C and at strain rates of 10?3, 100, and 103 s?1 to determine the mechanical properties. The α′‐martensite possesses high strength and surprisingly good ductility up to 60% of compressive strain. Using the flow stress behavior of the α′‐martensite and that of the stable austenitic steel AISI 316L, the flow stress behavior of the high alloyed CrMnNi TRIP‐steel is modeled successfully using a special RM proposed by Narutani et al. 相似文献
619.
Hans-Joachim Klaar Klaus Braag Radko Kaspar Josef Siegfried Distl Ulrike Zeislmair 《国际钢铁研究》1987,58(12):559-564
The influence of various reheating temperatures and times on austenite grain growth and dissolution behaviour of precipitates was investigated in three different microalloyed steels. The characteristics of these steels were compared with those of a non-microalloyed steel of similar basic composition. Then, the interrelationships between precipitate distribution, precipitate dissolution and austenite grain growth were discussed. 相似文献
620.
A total of 164 lactic acid bacteria (LAB) isolated from spoiled maatjes herring stored in air and under modified atmosphere at 4 or 10 degrees C were characterised and identified using an rRNA gene restriction pattern (ribotype) database. The isolates were initially grouped according to their HindIII restriction endonuclease profiles and further identified to species level using numerical analysis. Lactobacillus sakei, Lactobacillus curvatus and strains of the L. curvatus spp./Lactobacillus fuchuensis group were the main species detected. Of all the isolates, six were identified as Lactococcus spp. 相似文献