Cell Identification Codes for Tracking Mobile Users

The minimization of the wireless cost of tracking mobile users is a crucial issue in wireless networks. Some of the previous strategies addressing this issue leave an open gap, by requiring the use of information that is not generally available to the user (for example, the distance traveled by the user). For this reason, both the implementation of some of these strategies and the performance comparison to existing strategies is not clear. In this work we propose to close this gap by the use of Cell Identification Codes (CIC) for tracking mobile users. Each cell periodically broadcasts a short message which identifies the cell and its orientation relatively to other cells in the network. This information is used by the users to efficiently update their location. We propose several cell identification encoding schemes, which are used to implement different tracking strategies, and analyze the amount of information required by each tracking strategy. One of our major results is that there is no need to transmit a code which is unique for each cell. For example, a 3 bits CIC is sufficient to implement a distance-based tracking strategy in a two-dimensional system. In addition, we propose a combination of timer and movement tracking strategy, based on either a one-bit or a two-bit CIC, depending on system topology and user mobility. An important property of our framework is that the overall performance cost, and hence its comparison to existing methods, is evaluated for each tracking strategy. The CIC-based strategies are shown to outperform the geographic-based method currently used in existing networks, and the timer-based method, over a wide range of parameters. Moreover, this superiority increases as the number of users per cell increases.     

Shared-Memory Parallel Vector Implementation of the Immersed Boundary Method for the Computation of Blood Flow in the Beating Mammalian Heart

This paper describes the parallel implementation of the immersed boundary method on a shared-memory machine such as the Cray C-90 computer. In this implementation, outer loops are parallelized and inner loops are vectorized. The sustained computation rates achieved are 0.258 Gflops with a single processor, 1.89 Gflops with 8 processors, and 2.50 Gflops with 16 processors. An application to the computer simulation of blood flow in the heart is presented.     

Long Residence Times Revealed by Aurora A Kinase‐Targeting Fluorescent Probes Derived from Inhibitors MLN8237 and VX‐689

We report the development of three fluorescent probes for protein kinase Aurora A that are derived from the well‐known inhibitors MLN8237 and VX‐689 (MK‐5108). Two of these probes target the ATP site of Aurora A, and one targets simultaneously the ATP and substrate sites of the kinase. The probes were tested in an assay with fluorescence polarisation/anisotropy readout, and we demonstrated slow association kinetics and long residence time of the probes (k_on 10⁵–10⁷ M ^?1 s^?1, k_off 10^?3–10^?4 s^?1; residence time 500–3000 s). The presence of the Aurora A activator TPX2 caused a significant reduction in the on‐rate and increase in the off‐rate of fluorescent probes targeting ATP site. These observations were supported by Aurora A inhibition assays with MLN8237 and VX‐689. Overall, our results emphasise the importance of rational design of experiments with these compounds and correct interpretation of the obtained data.     

Perpendicular Orientation of Anisotropic Au‐Tipped CdS Nanorods at the Air/Water Interface

Anisotropic CdS nanorods tipped by Au nanoparticles on one edge (Au‐CdS‐NRs) are perpendicularly oriented at the air/water interface, whereby all the Au tips are located in the subphase, using the Langmuir–Blodgett technique. Since these nano‐objects reveal light‐induced charge separation at the semiconductor/metal interface, it is of high interest to control their organization. The orientation of these assemblies is studied in situ while compressing the Langmuir–Blodgett trough using the π‐A isotherm, Brewster angle microscopy, and horizontal touch voltammetry. All these analyses clearly confirm the induced organization of the amphiphilic Au‐CdS‐NRs by compression of the Langmuir layer. The compressed layers are successfully transferred by the Langmuir–Schaefer method onto transmission electron microscopy grids while maintaining the preferential orientation as analyzed by transmission, scanning and scanning trasmission electron microscopy, and X‐ray photoelectron spectroscopy. As far as can be determined, the Langmuir–Blodgett technique has not been used so far for perpendicularly orienting anisotropic nano‐objects. Moreover, these findings clearly demonstrate that anisotropic amphiphilic nano‐objects can be treated with some similarity to the traditional amphiphilic molecular building blocks.     

Nanoscale assembly in biological systems: from neuronal cytoskeletal proteins to curvature stabilizing lipids

The review will describe experiments inspired by the rich variety of bundles and networks of interacting microtubules (MT), neurofilaments, and filamentous-actin in neurons where the nature of the interactions, structures, and structure-function correlations remain poorly understood. We describe how three-dimensional (3D) MT bundles and 2D MT bundles may assemble, in cell free systems in the presence of counter-ions, revealing structures not predicted by polyelectrolyte theories. Interestingly, experiments reveal that the neuronal protein tau, an abundant MT-associated-protein in axons, modulates the MT diameter providing insight for the control of geometric parameters in bio- nanotechnology. In another set of experiments we describe lipid-protein-nanotubes, and lipid nano-tubes and rods, resulting from membrane shape evolution processes involving protein templates and curvature stabilizing lipids. Similar membrane shape changes, occurring in cells for the purpose of specific functions, are induced by interactions between membranes and proteins. The biological materials systems described have applications in bio-nanotechnology.     

Moving Particles Through a Finite Element Mesh

We present a new numerical technique for modeling the flow around multiple objects moving in a fluid. The method tracks the dynamic interaction between each particle and the fluid. The movements of the fluid and the object are directly coupled. A background mesh is designed to fit the geometry of the overall domain. The mesh is designed independently of the presence of the particles except in terms of how fine it must be to track particles of a given size. Each particle is represented by a geometric figure that describes its boundary. This figure overlies the mesh. Nodes are added to the mesh where the particle boundaries intersect the background mesh, increasing the number of nodes contained in each element whose boundary is intersected. These additional nodes are then used to describe and track the particle in the numerical scheme. Appropriate element shape functions are defined to approximate the solution on the elements with extra nodes. The particles are moved through the mesh by moving only the overlying nodes defining the particles. The regular finite element grid remains unchanged. In this method, the mesh does not distort as the particles move. Instead, only the placement of particle-defining nodes changes as the particles move. Element shape functions are updated as the nodes move through the elements. This method is especially suited for models of moderate numbers of moderate-size particles, where the details of the fluid-particle coupling are important. Both the complications of creating finite element meshes around appreciable numbers of particles, and extensive remeshing upon movement of the particles are simplified in this method.     

Synchronized External Pulsation for Improved Tolerance to Acceleration Stress: Model Studies and Preliminary Experiments

Synchronized external pulsation is proposed as a method to improve tolerance to acceleration stress. This technique uses a modified anti-G suit which is pressurized and depressurized synchronously with the heart cycle. The feasibility of the procedure has been studied using a computer model of the cardiovascular system which includes the effects of Gz stress, and contains simulations of baroreceptor control of heart rate and venous tone. Model predictions indicate that for unprotected subjects, carotid pressure at eye level (ophthalmic artery pressure) decreases to 20 mmHg (beginning of central light loss) at approximately +3.6 Gz. Applying standard anti-G suit pressure to the model increases this level to 5.3 Gz. When synchronized external pulsation of 2 psi is superimposed on the standard anti-G suit pressure, the tolerance to acceleration stress is further augmented by at least 0.9 G above the protection afforded by the standard anti-G suit alone. A set of preliminary experiments on human subjects to test the feasibility of using the technique in the high-G environment has also been carried out. The results under various protection modes compare favorably to the model predictions. Our results suggest that the computer model presented here is a useful tool for studying cardiovascular responses under +GZ stress. It also indicates that using synchronized external pulsation pressure superimposed on the standard anti-G suit pressure may offer extra protection to acceleration stress.     

Efficient non‐linear reanalysis of skeletal structures using combined approximations

Non‐linear reanalysis of large‐scale structures usually involves much computational effort, because the set of non‐linear equations must be solved repeatedly during the solution process. Various approximations that are often used for linear reanalysis are not sufficiently accurate for non‐linear problems. In this study, solution procedures based on the combined approximations approach are developed and compared in terms of efficiency and accuracy. Various path‐independent non‐linear analysis and reanalysis problems are considered, including material non‐linearity, geometric non‐linearity and buckling analysis. Numerical examples demonstrate the effectiveness of the procedures presented. It is shown that in various cases accurate results can be achieved efficiently. Copyright © 2007 John Wiley & Sons, Ltd.