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101.
Emna Ouni Sbastien Pyr dit Ruys Marie-Madeleine Dolmans Gaëtan Herinckx Didier Vertommen Christiani A. Amorim 《International journal of molecular sciences》2020,21(23)
Currently, the extracellular matrix (ECM) is considered a pivotal complex meshwork of macromolecules playing a plethora of biomolecular functions in health and disease beyond its commonly known mechanical role. Only by unraveling its composition can we leverage related tissue engineering and pharmacological efforts. Nevertheless, its unbiased proteomic identification still encounters some limitations mainly due to partial ECM enrichment by precipitation, sequential fractionation using unfriendly-mass spectrometry (MS) detergents, and resuspension with harsh reagents that need to be entirely removed prior to analysis. These methods can be technically challenging and labor-intensive, which affects the reproducibility of ECM identification and induces protein loss. Here, we present a simple new method applicable to tissue fragments of 10 mg and more. The technique has been validated on human ovarian tissue and involves a standardized procedure for sample processing with an MS-compatible detergent and combined centrifugation. This two-step protocol eliminates the need for laborious sample clarification and divides our samples into 2 fractions, soluble and insoluble, successively enriched with matrisome-associated (ECM-interacting) and core matrisome (structural ECM) proteins. 相似文献
102.
Hammons Joshua A. Tumey Scott J. Idell Yaakov Jeffries Jason R. 《JOM Journal of the Minerals, Metals and Materials Society》2020,72(1):176-186
JOM - Alpha-radiation damage in metals is a concern for long-term radioactive storage and systems that produce nuclear energy. Accurate prediction of irradiated material properties and failure... 相似文献
103.
Zakaryan D. A. Kartuzov V. V. Khachatryan A. V. 《Powder Metallurgy and Metal Ceramics》2015,54(3-4):210-214
Powder Metallurgy and Metal Ceramics - The theoretical strength of LaB6 and MeB2 borides and a eutectic LaB6–TiB2 system is calculated with quasiharmonic approximation for uniaxial strain in... 相似文献
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High Temperature - The temperature dependences of the viscosity of liquid Fe90B x Si(10 − x) alloys are studied in the mode of heating and subsequent cooling. The temperature dependences of... 相似文献
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V. I. Zubkov O. V. Kucherova I. N. Yakovlev A. V. Solomonov 《Russian Microelectronics》2015,44(3):203-209
An automated system for integrated electrophysical and optical studies of semiconductor nanoheterostructures, which operates in a wide temperature range from 15 to 475 K, is designed. The setup is intended to measure the temperature and frequency admittance and electroluminescence spectra of light-emitting diode and laser chips formed on substrates of diameter up to 50.2 mm, and the distribution of parameters over the wafer. The setup includes the closed-cycle helium cryogenic station, LCR meter, and temperature controller. The characterization results of nanoheterostructures with InGaN/GaN multiple quantum wells, which are used for creating highly efficient white and blue light-emitting diodes, are presented. 相似文献