全文获取类型
收费全文 | 1587篇 |
免费 | 7篇 |
国内免费 | 1篇 |
专业分类
电工技术 | 2篇 |
化学工业 | 91篇 |
金属工艺 | 15篇 |
机械仪表 | 9篇 |
建筑科学 | 7篇 |
矿业工程 | 3篇 |
能源动力 | 23篇 |
轻工业 | 7篇 |
石油天然气 | 7篇 |
无线电 | 32篇 |
一般工业技术 | 105篇 |
冶金工业 | 1263篇 |
自动化技术 | 31篇 |
出版年
2023年 | 5篇 |
2022年 | 5篇 |
2021年 | 13篇 |
2020年 | 9篇 |
2019年 | 5篇 |
2018年 | 6篇 |
2017年 | 5篇 |
2016年 | 6篇 |
2015年 | 4篇 |
2014年 | 2篇 |
2013年 | 15篇 |
2012年 | 7篇 |
2011年 | 7篇 |
2010年 | 16篇 |
2009年 | 11篇 |
2008年 | 9篇 |
2007年 | 7篇 |
2006年 | 5篇 |
2005年 | 12篇 |
2004年 | 7篇 |
2003年 | 3篇 |
2002年 | 8篇 |
2001年 | 4篇 |
2000年 | 5篇 |
1999年 | 35篇 |
1998年 | 341篇 |
1997年 | 246篇 |
1996年 | 175篇 |
1995年 | 124篇 |
1994年 | 65篇 |
1993年 | 81篇 |
1992年 | 17篇 |
1991年 | 26篇 |
1990年 | 15篇 |
1989年 | 14篇 |
1988年 | 14篇 |
1987年 | 9篇 |
1986年 | 6篇 |
1985年 | 10篇 |
1984年 | 9篇 |
1983年 | 7篇 |
1982年 | 10篇 |
1981年 | 16篇 |
1980年 | 16篇 |
1979年 | 7篇 |
1978年 | 12篇 |
1977年 | 50篇 |
1976年 | 100篇 |
1975年 | 6篇 |
1974年 | 4篇 |
排序方式: 共有1595条查询结果,搜索用时 0 毫秒
61.
Helicase II (uvrD gene product) and helicase IV (helD gene product) have been shown previously to be involved in the RecF pathway of recombination. To better understand the role of these two proteins in homologous recombination in the RecF pathway [recBCsbcB(C) background, we investigated the interactions between helD, uvrD and the following RecF pathway genes: recF, recO, recN and ruvAB. We observed synergistic interactions between uvrD ant the recF, recN, recO and recG genes in both conjugational recombination and the repair of methylmethane sulfonate (MMS)-induced DNA damage. No synergistic interactions were detected between helD and the recF, recO and regN genes when conjugational recombination was analyzed. We did, however, detect synergistic interactions between helD and recF/recO in recombinational repair. Surprisingly, the uvrD deletion completely suppressed the phenotype of a ruvB mutation in a recBCsbcB(C) background. Both conjugational recombination efficiency and MMS-damaged DNA repair proficiency returned to wild-type levels in the deltauvrDruvB9 double mutant. Suppression of the effects of the ruvB mutation by a uvrD deletion was dependent on the recG and recN genes and not dependent on the recF/O/R genes. These data are discussed in the context of two "RecF" homologous recombination pathways operating in a recBCsbcB(C) strain background. 相似文献
62.
63.
64.
65.
G Clementi M Amico-Roxas A Caruso VM Catena Cutuli A Prato S Maugeri E de Bernardis U Scapagnini 《Canadian Metallurgical Quarterly》1994,54(8):PL119-PL124
The anti-inflammatory activity of calcitonin gene-related peptide (CGRP) has been studied in cutaneous inflammation induced by croton oil (CO), arachidonic acid (AA), tetradecanoylphorbol acetate (TPA) or cantharidin (CA). Our results show that mouse ear inflammation induced by CO, AA or TPA is decreased by topical administration of CGRP, whereas that induced by CA is not affected. The dose-response and temporal analysis of CGRP effect show that the maximal activity is present at the dose of 30 pmol/ear and when administered 30 min after the irritating agent. Moreover, pretreatment with capsaicin is able to mimic the anti-inflammatory effect of exogenous CGRP, while simultaneous administration of CGRP and capsaicin produces a reduced response. Our results suggest that CGRP released from sensory. 相似文献
66.
Programmed cell death, or apoptosis, is a process of fundamental importance to cellular homeostasis in metazoan organisms (Ellis, R. E., Yuan, J., and Horvitz, H. R. (1991) Annu. Rev. Cell Biol. 7, 663-698). The caspase family of mammalian proteases, related to the nematode death protein CED-3, plays a crucial role in apoptosis and inflammation. We report here the isolation and characterization of a new caspase, tentatively termed ERICE (Evolutionarily Related Interleukin-1beta Converting Enzyme). Based on phylogenetic analysis, ERICE (caspase-13) is a member of the ICE subfamily of caspases which includes caspase-1 (ICE), caspase-4 (ICErel-II, TX, ICH-2), and caspase-5 (ICErel-III, TY). Overexpression of ERICE induces apoptosis of 293 human embryonic kidney cells and MCF7 breast carcinoma cells. Like other members of the subfamily, ERICE is not activated by the serine protease granzyme B, a caspase-activating component of cytotoxic T cell granules. Therefore, ERICE most likely does not play a role in granzyme B-induced cell death. ERICE, however, was activated by caspase-8 (FLICE, MACH, Mch-5), the apical caspase activated upon engagement of death receptors belonging to the tumor necrosis factor family. This is consistent with a potential role for ERICE in this receptor-initiated death pathway. 相似文献
67.
VM Borderie M Lopez A Lombet S Carvajal-Gonzalez C Cywiner L Laroche 《Canadian Metallurgical Quarterly》1998,39(8):1511-1519
PURPOSE: To assess the effects of two different concentrations of albumin in a cryoprotective solution and two freezing methods on human corneal keratocyte ctyopreservation. METHODS: Isolated keratocytes were used for cryopreservation. Solutions of 10% dimethylsulfoxide with either 2% or 10% human albumin were used as cryoprotective agents. Cells either were transferred directly into a -80 degrees C freezer (freezing rate, 2 degrees C/min) or were cooled in a programmed freezer (1 degrees C/min until -40 degrees C and then 10 degrees C/min), which resulted in four different cryopreservation protocols. Cells were stored at -80 degrees C, then were thawed at 37 degrees C, and subsequently were cultured. Keratocytes were studied by means of trypan blue staining, growth assay, apoptosis assays, transmission electron microscopy, and immunochemistry. RESULTS: The percentage of cells that were alive after thawing ranged from 80% to 99% by trypan blue staining and from 45% to 60% by flow cytometry. The ratio of the number of living cells at the end of primary culture after cryopreservation to that before cryopreservation was significantly (P=0.04) higher after direct transfer into the -80 degrees C freezer than after controlled-rate freezing, whereas the albumin concentration had no significant influence on this ratio (P=0.45). The percentage of apoptotic cells was significantly higher after cryopreservation than in the control group of noncryopreserved cells; more than 5% 24 hours after thawing. Cryopreservation did not modify the keratocyte ultrastructure. Fibroblast growth factor dramatically decreased the serum-induced cell expression of alpha smooth muscle actin, whereas cryopreservation had no influence on this cell expression. CONCLUSIONS: A freeze-thaw trauma, which was related to cryopreservation-induced cell apoptosis, was revealed during primary culture after thawing. Direct transfer into the -80 degrees C freezer resulted in better postcryopreservation growth in the culture than controlled-rate freezing. A change in albumin concentration from 2% to 10% did not affect the results. 相似文献
68.
AP Kuznetsov R Dando IP Shmelev AM Demidov BV Efimov VM Shubko 《Canadian Metallurgical Quarterly》1998,(4):520-528
The contents and distribution of natural (214Pb, 214Bi, 228Ac, and 208Tl) and technogenic (90Sr, 137Cs, and 60Co) radionuclides in the Kara Sea bottom sediments was analyzed by using the materials collected by R/V "Pomor" (Murmansk Marine Biological Institute). In 1994, no high (critical) concentrations of technogenic radioisotopes were found in the sea sediments, while the spots (regions) of elevated 137Cs content found in 1984 were not confirmed in 1994. It was proposed that the main sources of entry of technogenic radionuclides in the sea sediments in the last years are the flow of the Ob'-Yenisei waters and container burials of radioactive waste in the sea, which appeared to have been markedly corroded. The latter is confirmed by detection in some places of 60Co, which was not previously found in the sediments. 相似文献
69.
NM Nasybullina VP Zherdev VI Gunar VM Kopelevich GS Kozlova 《Canadian Metallurgical Quarterly》1997,60(6):52-54
The authors studied the permeability of the blood-brain barrier (CEB) to two nootropics: calcium ketogomopantothenate (KRA-Ca), a GABA derivative, and and calcium salt of oxybutyrate (OB-Ca), a derivative of GOBA. It was established that both preparations penetrate the CEB easily and are found in the brain at different intervals after their administration. However, essential differences in the distribution constant of these drugs were disclosed: KPA-Ca permeated the CEB more intensively and was accumulated in larger amounts in the late-term intervals. 相似文献
70.