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991.
Giuseppe Schiavone Florian Fallegger Xiaoyang Kang Beatrice Barra Nicolas Vachicouras Evgenia Roussinova Ivan Furfaro Sébastien Jiguet Ismael Seáñez Simon Borgognon Andreas Rowald Qin Li Chuan Qin Erwan Bézard Jocelyne Bloch Grégoire Courtine Marco Capogrosso Stéphanie P. Lacour 《Advanced materials (Deerfield Beach, Fla.)》2020,32(17):1906512
The convergence of materials science, electronics, and biology, namely bioelectronic interfaces, leads novel and precise communication with biological tissue, particularly with the nervous system. However, the translation of lab-based innovation toward clinical use calls for further advances in materials, manufacturing and characterization paradigms, and design rules. Herein, a translational framework engineered to accelerate the deployment of microfabricated interfaces for translational research is proposed and applied to the soft neurotechnology called electronic dura mater, e-dura. Anatomy, implant function, and surgical procedure guide the system design. A high-yield, silicone-on-silicon wafer process is developed to ensure reproducible characteristics of the electrodes. A biomimetic multimodal platform that replicates surgical insertion in an anatomy-based model applies physiological movement, emulates therapeutic use of the electrodes, and enables advanced validation and rapid optimization in vitro of the implants. Functionality of scaled e-dura is confirmed in nonhuman primates, where epidural neuromodulation of the spinal cord activates selective groups of muscles in the upper limbs with unmet precision. Performance stability is controlled over 6 weeks in vivo. The synergistic steps of design, fabrication, and biomimetic in vitro validation and in vivo evaluation in translational animal models are of general applicability and answer needs in multiple bioelectronic designs and medical technologies. 相似文献
992.
Anderson Veronese Odinei Marques Rafael Moreira Anna L. Belli Rafael S. Bisinotto Todd R. Bilby Francisco Peñagaricano Ricardo C. Chebel 《Journal of dairy science》2019,102(7):6624-6638
Genetic selection of dairy cattle in the United States has included reproductive traits (daughter pregnancy rate, DPR; heifer conception rate, HCR), which is believed to have partly contributed to halting the decline in reproductive performance. The objectives of the current study were to evaluate the association among genomic merit for DPR (GDPR) and HCR (GHCR) with estrous characteristics measured by an automated device. Holstein heifers (n = 1,005) were genotyped at 2 mo of age and were classified into quartiles (Q1 = lowest, Q4 = highest) according to the GDPR and GHCR values of the study population. At 10 to 11 mo of age, heifers were fitted with a collar that recorded activity and rumination and determined the occurrence of estrus according to changes in activity and rumination compared with the individual's baseline values. Estrous characteristics of spontaneous estruses (SPE) and PGF2α-synchronized estruses (PGSE) were recorded. Heifers had their estrous cycle synchronized with PGF2α and following detection of estrus received either artificial insemination or embryo transfer according to the herd's genetic selection program. Heifers in Q2 (17.7 ± 0.3 h) of GHCR tended to have longer SPE than heifers in Q4 (16.7 ± 0.3 h). The interaction between GDPR and GHCR was associated with the likelihood of activity peak (0 = no estrus, 100 = maximum activity) ≥80 at SPE because, among heifers in Q3 and Q4 of GHCR, those in Q1 of GDPR were less likely to have an activity peak ≥80. Heifers in Q1 and Q2 of GDPR had reduced hazard of estrus within 7 d of the first PGF2α treatment compared with heifers in Q4 of GDPR. Heifers in Q1 (16.1 ± 0.4 h) of GDPR had shorter PGSE than heifers in Q2 (17.6 ± 0.4 h) and Q4 (17.4 ± 0.4 h) and tended to have shorter PGSE than heifers in Q3 (17.4 ± 0.4 h). Rumination nadir on the day of PGSE was greater for heifers in Q1 (?30.1 ± 0.9 min/d) of GDPR compared with heifers in Q4 (?33.7 ± 0.9 min/d). Among heifers receiving only artificial insemination, those in Q1 of GHCR (adjusted hazard ratio = 0.65; 95% confidence interval = 0.48–0.88) became pregnant at a slower rate than heifers in Q4. Genomic merit for HCR was negatively associated with SPE but tended to be positively associated with hazard of pregnancy, whereas GDPR was positively associated with PGSE and hazard of estrus. Selection of dairy cattle for DPR and HCR may improve reproductive performance through different pathways, namely estrous characteristics and pregnancy establishment. 相似文献
993.
Muñoz Jairo Alberto Bolmaro Raúl E. Jorge Alberto Moreira Zhilyaev Alexander Cabrera José María 《Metallurgical and Materials Transactions A》2020,51(9):4674-4684
Metallurgical and Materials Transactions A - It is well known that a balance between the generation of low-angle and high-angle grain boundaries (LABG and HAGB) is achieved in materials undergoing... 相似文献
994.
In this work, we study the capacitive properties of gel-type natural abundant polymers without any dopants, mainly commercial gelatin and agar in deionized water. Here, we propose a facile fabrication of flexible, transparent, and planar electrolytic capacitors using these gel-type and indium tin oxide thin films as electrodes deposited on poly(ethylene terephthalate) substrates. Through cyclic voltammetry and galvanostatic charge–discharge techniques, we found that the devices show a specific capacitance in the order of the millifarads per gram, a specific power of ~10 mW cm−2, which is sufficient to active low-power devices, and a life cycle with nearly 100% efficiency after 1,000 cycles. We found that we do not need to add dopants that improve the ionic conductivity of the natural polyelectrolytes to obtain capacitances within the millifarads per gram. The flexibility of the capacitors was demonstrated by bending them, after which they exhibited the same electrochemical performance as the unbent devices. The optical transparency of the capacitors was measured by UV–V is spectroscopy showing a high transmittance in the visible region. 相似文献
995.
Tania Chacón-Ordóñez Reinhold Carle Ralf Schweiggert 《Journal of the science of food and agriculture》2019,99(7):3220-3239
The frequent consumption of carotenoid-rich foods has been associated with numerous health benefits, such as the supply of provitamin A. To exert these health benefits, carotenoids need to be efficiently liberated from the food matrix, micellized in the small intestine, taken up by the enterocytes and absorbed into the human blood stream. Enormous efforts have been made to better understand these processes. Because human studies are costly, labor-intense and time-consuming, the evaluation of carotenoid liberation and micellization at the laboratory scale using simulated in vitro digestion models has proven to be an important tool for obtaining preliminary results prior to conducting human studies. In particular, the liberation from the food matrix and the intestinal micellization can be mimicked by simulated digestion, yielding an estimate of the so-called bioaccessibility of a carotenoid. In the present review, we provide an overview of the carotenoid digestion process in vivo, the currently used in vitro digestion models and the outcomes of previous bioaccessibility studies, with a special focus on correlations with concomitantly conducted human studies. Furthermore, we advocate for the on-going requirement of better standardized digestion protocols and, in addition, we provide suggestions for the complementation of the acquired knowledge and current nutritional recommendations. © 2018 Society of Chemical Industry 相似文献
996.
Maintenance of proper intracellular concentrations of monovalent cations, mainly sodium and potassium, is a requirement for survival of any cell. In the budding yeast Saccharomyces cerevisiae, monovalent cation homeostasis is determined by the active extrusion of protons through the Pma1 H+-ATPase (reviewed in another chapter of this issue), the influx and efflux of these cations through the plasma membrane transporters (reviewed in this chapter), and the sequestration of toxic cations into the vacuoles. Here, we will describe the structure, function, and regulation of the plasma membrane transporters Trk1, Trk2, Tok1, Nha1, and Ena1, which play a key role in maintaining physiological intracellular concentrations of Na+, K+, and H+, both under normal growth conditions and in response to stress. 相似文献
997.
Daniel K. Y. Solaiman Richard D. Ashby Alberto Nuñez Nicole Crocker 《Journal of surfactants and detergents》2020,23(3):553-563
Pseudohyphozyma (formerly Rhodotorula) bogoriensis synthesizes long-chain-length (22-carbon chain) sophorolipids (22:0-SL) that are variously acetylated at the 6′- or/and 6″-carbons of the sophorose unit. In this paper, we describe a low-temperature crystallization protocol that preferentially separates the 6′-monoacetylated 22:0-SL (6′-Ac1-22:0-SL) from a parental mixture of 22:0-SL containing a majority (64.7%) of the 6′,6″-diacetylated moiety (6′,6″-Ac2-22:0-SL), as deduced from high-pressure-liquid-chromatography evaporative-light-scattering-detection (HPLC-ELSD) and LC/Q-TOF-MS analyses. Tensiometry measurements using the Wilhelmy plate method yielded minimum-surface-tension (SFTmin) and critical-micelle-concentration (CMC) values of 34.6 ± 1.0 mN m−1 and 0.014 mM, respectively, for 6′-Ac1-22:0-SL (CRYSTAL) fraction and 34.9 ± 1.0 mM m−1 (SFTmin) and 0.018 (CMC) for the hexane precipitate (Hx-PRCP) fraction containing a high concentration (89.2%) of the diacetylated 6′,6″-Ac2-22:0-SL after crystal removal. In contrast, the SFTmin and CMC of the well-studied 16-18 carbon (C16-18)-SL of Starmerella bombicola were 35–37.2 mN m−1 and 0.05-(>0.3) mM, respectively. Individually, the purified CRYSTAL and Hx-PRCP fractions exhibited a similar degree of strong growth-inhibition activity against Cutibacterium (formerly Propionibacterium) acnes as determined by an agar-plate zone of inhibition assay. Study on the growth inhibition of oral health-related bacteria, i.e., Streptococcus mutans and Lactobacillus acidophilus, showed that, depending on the bacteria and strains tested, the CRYSTAL fraction was either slightly better than or equally effective as the Hx-PRCP fraction in inhibiting cell growth. 相似文献
998.
999.
Francisco Campuzano-Bolarín Manuel Díaz-Madroñero Álvar-Ginés Legaz-Aparicio 《国际生产研究杂志》2020,58(12):3800-3820
This paper proposes a rolling horizon (RH) approach to deal with management problems under dynamic demand in planning horizons with variable lead times using system dynamics (SD) simulation. Thus, the nature of dynamic RH solutions entails no inconveniences to contemplate planning horizons with unpredictable demands. This is mainly because information is periodically updated and replanning is done in time. Therefore, inventory and logistic costs may be lower. For the first time, an RH is applied for demand management with variable lead times along with SD simulation models, which allowed the use of lot-sizing techniques to be evaluated (Wagner-Whitin and Silver-Meal). The basic scenario is based on a real-world example from an automotive single-level SC composed of a first-tier supplier and a car assembler that contemplates uncertain demands while planning the RH and 216 subscenarios by modifying constant and variable lead times, holding costs and order costs, combined with lot-sizing techniques. Twenty-eight more replications comprising 504 new subscenarios with variable lead times are generated to represent a relative variation coefficient of the initial demand. We conclude that our RH simulation approach, along with lot-sizing techniques, can generate more sustainable planning results in total costs, fill rates and bullwhip effect terms. 相似文献
1000.
AbstractGrasping and object manipulation is a key element of intelligent behavior. Many innovative cyberphysical systems involve some kind of object grasp and manipulation, to the extent that grasping has been recognized as a critical technology for the next generation industrial systems. In this survey, we aim to draw a broad landscape of applications and current research trends and topics relating to grasping techniques and tools. Applications range from biomedical and surgical to industrial warehouse pick and place tasks, covering a wide range of spatial scales, from micro to macro scales. The resources involved and research lines under development include the latest computational intelligence tools as well as the research on new materials and devices for sensing and actuation. 相似文献