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1.
Cagnon Christine; Valverde Viviane; Masson Jean-Michel 《Protein engineering, design & selection : PEDS》1991,4(7):843-847
A set of 11 expression vectors was constructed, each of themharbouring a cloning cassette under the control of the promoter.Some of these vectors enable expression of foreign proteinsin the cytoplasm, while others include a synthetic sequencecoding for a very efficient secretion signal sequence. Otherfeatures are an fl origin of replication (in plus or minus orientation)and a promoterup mutation that enhances the already very highlevel of expression from these vectors. With such a versatilevector family, cloning, sequencing and sitedirected mutagenesiscan be performed on the same vector, and the level of expressioncan be defined according to the specific constraints of a givenprotein. 相似文献
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Carina F. Dorneles Marcos Freitas Nunes Carlos A. Heuser Viviane P. Moreira Altigran S. da Silva Edleno S. de Moura 《Information Systems》2009,34(8):673
Approximate data matching aims at assessing whether two distinct instances of data represent the same real-world object. The comparison between data values is usually done by applying a similarity function which returns a similarity score. If this score surpasses a given threshold, both data instances are considered as representing the same real-world object. These score values depend on the algorithm that implements the function and have no meaning to the user. In addition, score values generated by different functions are not comparable. This will potentially lead to problems when the scores returned by different similarity functions need to be combined for computing the similarity between records. In this article, we propose that thresholds should be defined in terms of the precision that is expected from the matching process rather than in terms of the raw scores returned by the similarity function. Precision is a widely known similarity metric and has a clear interpretation from the user's point of view. Our approach defines mappings from score values to precision values, which we call adjusted scores. In order to obtain such mappings, our approach requires training over a small dataset. Experiments show that training can be reused for different datasets on the same domain. Our results also demonstrate that existing methods for combining scores for computing the similarity between records may be enhanced if adjusted scores are used. 相似文献
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Viviane Torres Da Silva Carlos J. P. De Lucena 《Autonomous Agents and Multi-Agent Systems》2004,9(1-2):145-189
In this paper we propose a multi-agent system modeling language (MAS-ML) that extends UML (Unified Modeling Language) based on structural and dynamic properties presented in a conceptual framework (metamodel) called Taming Agents and Objects (TAO). Our focus is the adaptation according to TAO concepts of the static Class diagram and the interaction Sequence diagram proposed by UML. These two diagrams have been chosen because they are the most commonly used and because it is possible to use them to illustrate both the structural and dynamic aspects of TAO metamodel. We propose a conservative extension of the UML metamodel, which includes agent-related notions that are part of the TAO conceptual framework while preserving all object-related concepts, which constitute the UML metamodel. The main difference between our approach and the others presented in the literature is the clear definition and representation of the elements that compose MASs and their corresponding behavior. In order to extend UML with TAO non-object concepts, it is not possible to use only the three basic extensions mechanisms provided by UML and new metaclasses and stereotypes have been created and associated with the UML metamodel. 相似文献
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João P. F. Mota Viviane G. P. Ribeiro Fernando L. F. da Silva Antônio E. C. Junior Davi R. Oliveira Lloyd R. V. Kotzebue 《分离科学与技术》2016,51(14):2473-2483
The present work made a comparative study between two purification methods, column chromatography and recrystallization, for compounds derived from cardanol, a by-product of the cashew industry (Anacardium occidentale L.). The compounds were successfully synthesized and characterized, focusing our attention to results obtained in terms of yield, purity, generation of solid waste and amount of solvent used in each purification process. For all the synthesized compounds, the amount of solvent used was greatly reduced in the recrystallization process, with purities above 80%, when compared to chromatographic column, which still demands high amounts of eluent. 相似文献
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Viviane Muniz da Silva Fragoso Carla Patrícia de Morais Coura Luanda Yanaan Hoppe Marília Amável Gomes Soares Dilson Silva Celia Martins Cortez 《International journal of molecular sciences》2016,17(1)
The aim of this work was to study the interaction of sulpiride with human serum albumin (HSA) and bovine serum albumin (BSA) through the fluorescence quenching technique. As sulpiride molecules emit fluorescence, we have developed a simple mathematical model to discriminate the quencher fluorescence from the albumin fluorescence in the solution where they interact. Sulpiride is an antipsychotic used in the treatment of several psychiatric disorders. We selectively excited the fluorescence of tryptophan residues with 290 nm wavelength and observed the quenching by titrating HSA and BSA solutions with sulpiride. Stern-Volmer graphs were plotted and quenching constants were estimated. Results showed that sulpiride form complexes with both albumins. Estimated association constants for the interaction sulpiride–HSA were 2.20 (±0.08) × 104 M−1, at 37 °C, and 5.46 (±0.20) × 104 M−1, at 25 °C. Those for the interaction sulpiride-BSA are 0.44 (±0.01) × 104 M−1, at 37 °C and 2.17 (±0.04) × 104 M−1, at 25 °C. The quenching intensity of BSA, which contains two tryptophan residues in the peptide chain, was found to be higher than that of HSA, what suggests that the primary binding site for sulpiride in albumin should be located next to the sub domain IB of the protein structure. 相似文献
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de Oliveira Amanda Gerhardt Moreno Janaina Fernandes de Sousa Ana Maria Furtado Esccio Viviane Alves de Oliveira Cavalcanti Guimares Maria Jos da Silva Ana Lucia Nazareth 《Polymer Bulletin》2020,77(6):2889-2904
Polymer Bulletin - Investigating the compatibility mechanism of hybrid composites based on two polymers and one mineral nanofiller is a challenge that needs to be better understood. This study... 相似文献
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El‐Hajjaji Soundous Grard Amaury De Laubier Juliette Di Tanna Sybille Lain Aurlie Patz Viviane Sindic Marianne 《International Journal of Dairy Technology》2019,72(3):466-471
This study aims to describe the procedures and practices used in local production of raw milk butter. The demand for local products is increasing; hence, there is a need to describe the practices used in the artisanal production of raw milk butter. Therefore, a survey of 147 raw milk butter producers was carried out. The results from the survey indicate that there is not one single way to produce butter at artisanal level. In terms of maturation, six temperature sequences were distinguished. Attention is required at every step of production starting from breeding. 相似文献