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941.
W Klinger A Lupp E Karge H Oelschl?ger D Rothley M Danz W Linss S L?bel T Deufel M Müller M Klinger W Kühnel 《Canadian Metallurgical Quarterly》1998,50(4-6):301-314
Ciprofibrates (racemate and both enantiomers, Raccip, R- and Scip) were administered orally in doses of 1 and 10 mg/kg once daily over 28 days to male inbred Fischer 344 rats, age 90-110 days at the beginning of the experiment. Body mass gain was observed in all groups. The 1 mg groups showed almost no difference to the control group. The 10 mg groups exhibited less body mass gain, most pronounced in the Scip group. Liver masses were increased in a dose dependent manner up to more than 200%, only the 10 mg Scip group was not significantly different from the 1 mg group which exhibited an increase in liver weight to about 175%. Also the kidney weights increased to 130%, whereas thymus and spleen weights were decreased in the high dose groups. Liver microsomal cytochromes P450 (P450) concentrations were not altered in the 1 mg groups and distinctly lowered in the 10 mg groups. Ethoxyresorufin and ethoxycoumarin O-deethylations were lowered in all experimental groups in a dose dependent manner, after administration of the high doses down to 30% of the control levels or less. Pentoxyresorufin O-depentylation, however, was increased in all 1 mg groups. In the high dose groups it was not altered. Ethylmorphine N-demethylation was decreased after administration of the high doses by about 50%, but only Scip decreased this reaction also after administration of the low dose. NADPH/Fe2+-stimulated microsomal luminol and lucigenin amplified chemiluminescence was increased, whereas hydrogen peroxide formation was depressed even by the low doses to 50% of the normal values, to about 25% by the high doses. Microsomal lipid peroxidation, however, was only slightly or not influenced. Glutathion concentrations (in the reduced and the oxidized form) were increased in a dose dependent manner by about 20 to 30%, the concentration of lipid peroxides was not significantly influenced. Thus, the effects of the enantiomers were not different and were similar to those of the racemate. In serum, cholesterol and triglycerides were only moderately lowered. Albumin concentrations were significantly enhanced in all groups, total proteins after 1 mg/kg Raccip only. Serum bilirubins were not altered, and among the indicator enzymes for liver damage only ALAT, alkaline phosphatase and the dehydrogenases were increased, in no case higher than twofold. Histologically distinct effects were seen after administration of both doses, more pronounced after 10 mg/kg, but with no differences between the enantiomers and Raccip: marked hypertrophy of the hepatocytes, reduced staining of the nuclei, strongly acidophilic granulated cytoplama, no basophilia of the cell bodies, loss of glycogen. These changes were most pronounced around the central veins. Hepatocyte apoptoses also were observed. By immunohistochemistry an increased staining was seen for all P450 isoforms tested (1A1, 2B1, 2E1, 3A2 and 4A1), predominantly perivenously and most pronounced after administration of the high doses without differences between Rcip, Scip or Raccip (preliminary results). By electron microscopy a moderate proliferation of peroxisomes after treatment with 1 mg/kg Cips with a ratio between mitochondria and peroxisomes of about 1:1 (controls: 10:1) was observed, and the peroxisomes were a more heterogeneous population. The relative portions of glycogen and both forms of the ER decreased. Treatment with 10 mg/kg Rcip, Scip or Raccip led to a strong increase in the number of peroxisomes, in some hepatocytes the ratio between mitochondria and peroxisomes was 1:3 with an increased heterogeneity among the peroxisomes evidenced by a broad range of electron densities. Most peroxisomes lacked a nucleoid. Thus, the biochemical effects differed only slightly and the morphological effects of the enantiomers were not different and were similar to those of the racemate. 相似文献
942.
The UvrA and UvrB proteins form part of the UvrABc endonuclease, which is responsible for nucleotide excision repair in Escherichia coli. Using a mobility shift gel assay we have studied the binding of UvrA dimer, UvrB monomer and UvA(2)B trimer complexes with 40, 50 and 136 bp (32)P-end-labelled DNA fragments adducted with aflatoxin B(1). UvrA was shown to re-associate with adduct specific UvrB: DNA complexes, a phenomenon which could be reversed by the addition of 500 mM potassium chloride or anti-UvrA anti-sera. Re-association was shown to be UvrA concentration dependent. Re-association of UvrA(2)B to the UvrB:DNA complex was not seen. We have also shown that the UvrB:DNA complex, in the case of aflatoxin B(1), is extremely stable with a half-life excess of 400 min and that fragment termini are not a specific substrate for UvrA binding. 相似文献
943.
ART-EMAP: A neural network architecture for object recognition byevidence accumulation 总被引:1,自引:0,他引:1
A new neural network architecture is introduced for the recognition of pattern classes after supervised and unsupervised learning. Applications include spatio-temporal image understanding and prediction and 3D object recognition from a series of ambiguous 2D views. The architecture, called ART-EMAP, achieves a synthesis of adaptive resonance theory (ART) and spatial and temporal evidence integration for dynamic predictive mapping (EMAP). ART-EMAP extends the capabilities of fuzzy ARTMAP in four incremental stages. Stage 1 introduces distributed pattern representation at a view category field. Stage 2 adds a decision criterion to the mapping between view and object categories, delaying identification of ambiguous objects when faced with a low confidence prediction. Stage 3 augments the system with a field where evidence accumulates in medium-term memory. Stage 4 adds an unsupervised learning process to fine-tune performance after the limited initial period of supervised network training. Each ART-EMAP stage is illustrated with a benchmark simulation example, using both noisy and noise-free data. 相似文献
944.
W Creutzfeldt 《Canadian Metallurgical Quarterly》1996,20(2):126-131
In this communication we report 4 cases of gastric volvulus seen at the Department of Surgery University College Hospital, Ibadan, over a four-year period giving a hospital incidence of one case per year. The often difficult diagnosis and mode of management is presented along with a review of world literature. One of our cases is the fifth case of combined organomesenteroaxial volvulus in a child reported in world literature. 相似文献
945.
Plaice J. Wadge W.W. 《IEEE transactions on pattern analysis and machine intelligence》1993,19(3):268-276
A method for controlling versions of software and other hierarchically structured entities is presented. Using the variant structure principle, a particular version of an entire system is formed by combining the most relevant existing versions of the various components of the system. An algebraic version language that allows histories (numbered series), subversions (or variants), and joins is described. It is shown that the join operation is simply the lattice least upper bound and together with the variant structure principle, provides a systematic framework for recombining divergent variants. The utility of this approach is demonstrated using LEMUR, a programming environment for modular C programs, which was developed using itself. The ways in which this notion of versions is related to the possible world semantics of intensional logic are discussed 相似文献
946.
We study tandem queues with finite intermediate buffers and develop a general blocking scheme that unifies and generalizes various blocking schemes previously studied in the literature, such as manufacturing blocking, communication blocking, kanban blocking and its variations. In the general blocking scheme, job movement and service at each stage are controlled by three parameters, which represent, respectively, the upper limits (at that stage) on work-in-process inventory, finished goods inventory, and buffer space. We derive a set of recursive equations that characterize the dynamics of the system, in terms of the job completion and departure processes, and establish comparison results for these processes in the settings of stochastic ordering, variability ordering and stochastic convexity. Free of distributional assumptions, the results provide characterization of system behavior with respect to the control parameters as well as to the arrival and service processes. We also compare system performance under two different modes of operation: make-to-order versus make-to-stock, and demonstrate the trade-off between improving service and reducing inventory. Numerical studies are also presented to illustrate the diversity of performance trade-off, including composition of inventory, offered by the general model.Supported in part by NSF under grant ECS-89-96201. 相似文献
947.
The finite element method is applied to stress and strain analyses around rigid spherical particles in elastomers at large extensions. The stress and strain distribution computed agree well with the classical theoretical ones at small strain. At large extension, however, the maximum stress concentration factor increases greatly and the maximum strain concentration factor decreases slightly as strain increases. These tendencies are increased more in carbon black-filled elastomers than in unfilled ones, which can be understood reasonably by considering both the geometric and material non-linearity. Reinforcement of elastomers with rigid spherical particles was also analysed through a numerical computation. The computed results agree with the Guth and Mooney equations at low volume fraction of fillers. In carbon black-filled elastomers, on the other hand, where the modulus is much higher than that given by the above equations, the computations give a good agreement with the experiments, considering the 20% increase in effective diameter of the filler. 相似文献
948.
Cooking reduces odor intensity in boar meat but also may induce lipid oxidation unless the meat pH is above approximately 6.0. This research was designed to determine the feasibility of cooking boar meat in the prerigor state to overcome boar odor and lipid oxidation problems. Prerigor and postrigor triceps brachii muscle samples from 40 boars (20 Duroc and 20 Yorkshire) were cooked to 60 degrees C, frozen and stored at -20 degrees C, reheated in a 60 degrees C water bath for 1 h, and then subjected to pH, thiobarbituric acid (TBA), and sensory analyses. Boar odor intensity and skatole concentration in backfat samples were determined by olfactory test and HPLC, respectively. Cooked (initial cooking) prerigor meat was found to have higher (P < .05) pH and lower (P < .05) TBA values than comparable postrigor meat (6.44 vs 6.09 and 2.15 vs 3.23, respectively). Regression analysis indicated an inverse relationship between pH and TBA values (r = -.52; P < .01). No appreciable changes in TBA values were noted after frozen storage for 14 to 98 d, but reheating increased TBA values (P < .05) in both prerigor and postrigor samples (3.45 vs 4.32, respectively). Sensory evaluation scores indicated that prerigor cooked meat was less tender with more pronounced rancid flavor than postrigor cooked meat (P < .05), but panelists may have allowed the toughness of the prerigor samples to adversely affect their flavor scores. No difference in boar odor was detected between rigor states or breeds. Mean skatole concentration in backfat was .12 micrograms/g and no difference was detected between breeds.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
949.
950.
WL Flejter D Finlinson S Root W Nguyen AR Brothman D Viskochil 《Canadian Metallurgical Quarterly》1996,66(3):276-280
Ring (19) chromosomal mosaicism has been identified in a 14-month-old girl referred for cytogenetic evaluation due to microcephaly and developmental delay with autistic-like mannerisms. An analysis of her peripheral blood lymphocytes showed a 46,XX,r(19) cell line in 119/121 of cells examined. Of the two remaining cells, one had a normal female chromosome complement and the other showed loss of one of the chromosome 19 homologs. Further analysis by fluorescence in situ hybridization using an all human telomere probe showed the presence of a single hybridization signal on the r(19) chromosome. Subsequent cytogenetic characterization of cells derived from the patient's phenotypically normal mother also demonstrated the presence of a ring 19 chromosome in 4/100 cells. The remaining cells had a normal female chromosome complement. These findings represent the first reported case of familial ring 19 mosaicism. The cytogenetic and clinical findings in these two individuals are discussed in relation to six previously reported cases of de novo ring chromosome 19 mosaicism. 相似文献