Endoscopic study of the small intestine with push enteroscopy. A prospective study

OBJECTIVE: To evaluate whether push enteroscopy of the small bowel (PES), undertaken after extensive previous investigations in suspected intestinal bleeding from an uncertain site, chronic diarrhoea or lymphoma of the small intestine, contributes to the diagnosis, and to ascertain the results of PES and its clinical significance. PATIENTS AND METHODS: 56 consecutive patients (29 men, 27 women; mean age 63 years) were investigated prospectively. The main indications for PES were the search for the source of intestinal bleeding in 79% of patients (group A), chronic diarrhoea or tropical sprue in 16% (group B) and search for tumour of lymphoma in 5% (group C). PES was always performed in fasting patients under sedation/analgesia using a video PES, which contrary to catheter enteroscopy provides a channel for intervention. RESULTS: In group A 27% of patients were found to have lesions, in particular angiodysplasias, or (in once case) leiomyoma. Half of these patients were successfully treated endoscopically without later surgical intervention being required (mean follow-up of six months). In the other half operation became necessary, either because the disease itself indicated it or the bleeding persisted, the source being in the more distant small intestine and thus not accessible to endoscopic intervention: only 50% of the length of the small intestine proved to be within reach of the instrument. No abnormalities were discovered in patients of groups B and C. CONCLUSION: In cases of gastrointestinal bleeding from an uncertain source PES should be performed first, because in many cases it may obviate surgical intervention. But PES seems to contribute little of diagnostic value in other indications.     

Pathological staging and biochemical recurrence after neoadjuvant androgen deprivation therapy in combination with radical prostatectomy in clinically localized prostate cancer: results of a phase II study

OBJECTIVES: To assess the pathological staging and biochemical progression-free survival (assessed using serum prostate-specific antigen level) of patients with clinically localized prostate cancer using neoadjuvant androgen deprivation therapy (ADT) in combination with radical retropubic prostatectomy (RRP). PATIENTS AND METHODS: A prospective study was carried out on 69 patients with localized prostate cancer who were enrolled in a trial of 3 months of ADT followed by RRP (group 1). These patients were compared with 72 patients matched for age and clinical stage who declined ADT therapy and had RRP concurrently (group 2). Assignment to the individual treatment groups was thus determined by the patient's preference and not the physician's selection. Pathological staging and biochemical progression-free recurrence were compared between the groups. RESULTS: The rate of organ-confined (pT2) tumours was 74% in group 1 and 49% in group 2 (P < 0.01), and the rate of margin-negative tumours was 87% in group 1 and 64% in group 2 (P < 0.01). Within a median follow-up of 35 months, there was no significant difference in biochemical failure between the groups (P = 0.37). Patients with pT2 disease, regardless of treatment, had similar biochemical failure rates. In the patients with margin-positive disease, there was a significantly higher biochemical failure rate in group 1 (P = 0.02). CONCLUSIONS: The rates of organ- and specimen-confined disease were higher among the patients treated with ADT. The preliminary follow-up suggested that patients with pT2 disease after ADT have a biochemical progression-free recurrence rate similar to pT2 patients treated with RRP alone. Additionally, high biochemical failure rates in patients with margin-positive disease after ADT may identify a subset of more biologically aggressive tumours in need of early adjuvant treatment.     

Design and Testing of a Graphite Foam-Based Supercooler for High-Heat-Flux Cooling in Optoelectronic Packages

The feasibility of using graphite foam as a heat sink and heat spreader in optoelectronic packages is assessed. A “supercooler” is designed, fabricated, and tested to verify its cooling capability under high heat flux conditions in a typical optoelectronic package. The supercooler uses graphite foam as a primary heat transfer material. Water is soaked into the graphite foam, and under evacuated pressure, boiling is initiated under the heating region to provide enhanced cooling. Experiments were conducted for a heat flux of up to 400 W/cm² deposited over a heating area of 0.5 mm × 5 mm. Two-dimensional transient temperature distributions were recorded using a high-speed infrared camera. Data were obtained for steady heating, and for periodic heating with frequency up to 8 Hz. Results show that the supercooler is very efficient in dissipating heat away from the heating region. The average cooling rate during the cooling period exceeds 170 K/s.     

Performance of an 8×8 LiNbO3switch matrix as a gigahertz self-routing switching node

The performance of an LiNbO3 integrated-optic crossbar switch as a node in a gigahertz self-routing network is measured. Switch throughput supports 12.5 Gbit/s signals with a measured switching speed of 1.33 GHz. Crosstalk due to RF/ acousto-optic coupling and modulation depth are reported at 1.33 GHz. Optical self-routing of l00 Mbit/s information using the 8×8 switch is demonstrated.     

Crystal structures of a marginally active thymidylate synthase mutant, Arg 126-->Glu

Thymidylate synthase (TS) is a long-standing target for anticancer drugs and is of interest for its rich mechanistic features. The enzyme catalyzes the conversion of dUMP to dTMP using the co-enzyme methylenetetrahydrofolate, and is perhaps the best studied of enzymes that catalyze carbon-carbon bond formation. Arg 126 is found in all TSs but forms only 1 of 13 hydrogen bonds to dUMP during catalysis, and just one of seven to the phosphate group alone. Despite this, when Arg 126 of TS from Escherichia coli was changed to glutamate (R126E), the resulting protein had kcat reduced 2000-fold and Km reduced 600-fold. The crystal structure of R126E was determined under two conditions--in the absence of bound ligand (2.4 A resolution), and with dUMP and the antifolate CB3717 (2.2 A resolution). The first crystals, which did not contain dUMP despite its presence in the crystallization drop, displayed Glu 126 in a position to sterically and electrostatically interfere with binding of the dUMP phosphate. The second crystals contained both dUMP and CB3717 in the active site, but Glu 126 formed three hydrogen bonds to nearby residues (two through water) and was in a position that partially overlapped with the normal phosphate binding site, resulting in a approximately 1 A shift in the phosphate group. Interestingly, the protein displayed the typical ligand-induced conformational change, and the covalent bond to Cys 146 was present in one of the protein's two active sites.     

The λ-scheduler: A multiwavelength scheduling switch

We propose a new multiwavelength almost all-optical switch architecture called the λ-scheduler that uses wavelength division multiplexing (WDM) internally to fold the switch architecture in both the space and time domains to reduce the hardware complexity and to improve the signal characteristics through the switch. The λ-scheduler preserves the packet order for a given input-output pair, is consistent with virtual circuit switching, and when combined with appropriate connection and flow control protocols, provides lossless communication for bursty (or nonconstant rate) traffic, provided the traffic satisfies certain smoothness properties. The λ-scheduler uses novel scheduling and wavelength assignment algorithms, in conjunction with a series of feed-forward delay blocks, to avoid packet collisions within the switch or at the switch outputs. We present two implementations of the λ-scheduler when the number of internal wavelengths k equal the number of inputs (and outputs) N to the switch. In the compressed λ-scheduler, the N internal wavelengths are used to fold the architecture in the time domain, which reduces the total number of delay blocks for the switch by 2N log N. In the collapsed λ-scheduler, the N internal wavelengths are used to fold the architecture in the space domain, which reduces the number of delay blocks and total fiber length used for delays by a factor of N. We examine the insertion loss for both λ-scheduler implementations and discuss the trade-offs between the reduction in overall component count and the improvement in the signal characteristics