Feasibility study on the use of polyallyldiglycol-carbonate cell dishes in TUNEL assay for alpha particle radiobiological experiments

In the present work, we have studied the feasibility of a method based on polyallyldiglycol-carbonate (PADC) films to investigate the effects of alpha particles on HeLa cervix cancer cells. Thin PADC films with thickness of about 20 μm were prepared from commercially available CR-39 films by chemical etching to fabricate custom-made petri dishes for cell culture, which could accurately record alpha particle hit positions. A special method involving “base tracks” for aligning the images of cell nuclei and alpha particle hits has been proposed, so that alpha particle transversals of cell nuclei can be visually counted. Radiobiological experiments were carried out to induce DNA damages, with the TdT-mediated dUTP Nick-End Labeling (TUNEL) fluorescence method employed to detect DNA strand breaks. The staining results were investigated by flow cytometer. The preliminary results showed that more strand breaks occurred in cells hit by alpha particles with lower energies. Moreover, large TUNEL positive signals were obtained even with small percentages of cells irradiated and TUNEL signals were also obtained from non-targeted cells. These provided evidence for the bystander effect.     

新经济增长理论对我国人力资本发展的借鉴

新经济增长理论为人力资本理论注入了新的内容,为各国如何利用人力资本发展经济提供了理论指导。分析了新经济增长理论中有关人力资本的理论,以此对我国经济和人力资本的发展提出了一些借鉴;收益递增模型。     

Integrated Personal Mobility Architecture: A Complete Personal Mobility Solution

The high expectations and demand for users to access the Internet from anywhere at anytime has made user mobility an important part of the design and development of the next generation mobile communications and computing. Traditionally user mobility has been divided into two areas: Terminal Mobility and Personal Mobility. In recent years terminal mobility has focused on the movement of the terminal and developed extensions to IP protocols such as Mobile IP. In contrast, personal mobility has only received limited attention, and is somewhat lagging behind. This research has either focussed on personal mobility in communications or personalisation of operating environments. As a result, to date no framework for providing true personal mobility has emerged. In this paper, we introduce a new personal mobility framework called IPMoA (Integrated Personal Mobility Architecture), which integrates both aspects of personal mobility to provide a complete personal mobility solution, and illustrate the viability of this approach through a proof-of-concept implementation.     

Copolymerization of bromophenylmaleimide with ethyl or butyl methacrylate

N‐p‐Bromophenylmaleimide (BrPMI) does not polymerize in solution by conventional free radical mechanism. However, it readily polymerized in bulk when mixed with a free radical initiator and heated in a microwave oven for 7–8 min. Copolymerization of ethyl methacrylate or butyl methacrylate with BrPMI was conducted in dioxane. The copolymers were characterized by IR and ¹H NMR spectroscopy and gel permeation chromatography. The monomer reactivity ratios were calculated by a non‐linear least‐square analysis. Thermal analysis indicated a great improvement in thermal stability of the copolymers compared with the methacrylate homopolymers. BrPMI was also polymerized in bulk in the DSC pan, which allowed the calculation of the activation energy of its polymerization. Copyright © 2003 Society of Chemical Industry     

A study on the anodic protection of Titanium evaporators in Al2(SO4)3 solution

The feasibility of the anodic protection of titanium evaporators in Al₂(SO₄)₃ solution was studied by the measurement of polarization curves, weight loss, solubility of passive film and AC impedance. The protection parameters and efficiency were determined. In addition, the practical technology of anodic protection was studied by screening reference electrodes and auxiliary cathodes. Throwing power was also measured. Four rows of tube-type evaporators of titanium have been anodically protected in situ. Field tests lasting more than one year show satisfactory results.     

Three‐dimensionally braided carbon fiber–epoxy composites,a new type of material for osteosynthesis devices. I. Mechanical properties and moisture absorption behavior

In recent years, three‐dimensionally (3D) braided composites have attracted a great deal of attention because of their high‐impact damage tolerance and fatigue life, superior fracture toughness, and so forth, and have been used in aeronautics, military, and transportation. These advantages make them strong candidates for osteosynthesis devices. In this study, 3D braided carbon fiber–epoxy (C_3D/EP) composites were produced via a simple vacuum impregnation technique. The load‐deflection curve, mechanical properties, and influence of fiber volume fraction, braiding angle, and axial reinforcing fibers were examined to determine their suitability for internal fixation devices. It is found that the C_3D/EP composites have excellent toughness and do not show brittleness when fractured because of their relatively high void content. The flexural, shear, and impact strengths of the C_3D/EP composites are excellent. It was shown that a C_3D/EP composite with a stiffness similar to load‐bearing bones can be made while maintaining enough strength. It is concluded that a relatively higher void content and braiding angle is more suitable for the C_3D/EP composites from the viewpoint of requirements of fracture fixation materials. The moisture absorption behavior and changes in mechanical properties caused by moisture uptake were evaluated. Results show that absorbed moisture slightly decreases mechanical properties of the C_3D/EP composites. Contrary to the unreinforced epoxy, the moisture absorption behavior of the C_3D/EP composites cannot be described with Fick's law of diffusion, probably because of the presence of voids and/or 3D fiber structure. The exact mechanisms should be proposed in further investigations. © 2002 Wiley Periodicals, Inc. J Appl Polym Sci 85: 1031–1039, 2002     

圆锥形零件冲压变形与成形载荷的分析

由于悬空侧壁部分的变形状态是圆锥形零件成形的关键，而径向拉应力是实现悬空部分成形的必要条件，同时也是该部分冲压成形成败的关键为此，经力学分析得出了侧壁部分径向拉应力的解析式和造成圆锥形零件破裂的最大径向拉应力的计算式。同时分析了圆锥形零件的成形载荷。     

塑钢包装箱材料贮存寿命预测

用热老化箱对4种塑钢包装箱用材进行了为期1个月的热老化试验，以材料的冲击强度作为判据指标，通过回归处理外推得到其常温下的贮存寿命预测评估结果。     

新抗生素B-3543的研究

对新抗生素B-3543进行了系统的研究,关键指标已达到工业化生产水平。确立了产生菌的分类地位,测定了抗生素的抗菌谱,获得效价达10000μg/ml的高产菌株,阐明了B-3543的化学结构,提出3种效价检定方法,制定了产品质量标准,完善了生产工艺,使产品总收率达60％。该项目还存在产品成本过高,产品稳定性不好等问题,有待研究解决。