Profile of polyunsaturated fatty acids produced by Thraustochytrium sp. KK17-3

More than 300 strains of microorganisms producing polyunsaturated fatty acids (PUFA) were newly isolated from coastal seawater in the Seto Inland Sea and around Iriomote Island, Japan, by the baiting method. The profiles of PUFA from docosahexaenoic acid (DHA)-producing strains could be classified into four types. A strain, named KK17-3, was chosen for further study owing to its high DHA content (52.1% of total fatty acid) and wide range of PUFA (76.1%) including arachidonic, eicosapentaenoic, and docosapentaenoic acids as well as DHA. Glucose and tryptone were the optimal carbon and nitrogen sources, respectively, in a medium with salinity at 75% that of seawater. The PUFA contents in polar lipids (22.1% of total lipid), in which the DHA content was 39.3%, were higher than those in neutral lipids and glycolipids. Molecular phylogenetic analysis of 18S rRNA gene sequences showed KK17-3 to be a thraustochytrid. It also was observed to possess a life cycle composed of vegetative cells without successive bipartition, zoosporangium, and zoospore stage. Classification by the chemotaxonomic criterion based on PUFA compositions also supported this assignment.     

Optical guiding of intense laser pulses by fast Z-pinch discharge

We have proposed the optical guiding of intense laser pulses by fast Z-pinch for channel-guided laser wakefield acceleration (LWFA). The method has been developed based on capillary discharge-pumped X-ray laser technique. A discharge through preionized helium gas driven by a current of 4.8 kA with a rise time of 15 ns proved able to produce a uniform guiding channel with good reproducibility, less than the time jitter of 1.8 ns. The observed guiding channel formation process was corroborated by 1D-MHD simulation. With this new guiding method, an intense Ti-sapphire laser pulse (λ = 790 nm, 2.2 TW, 90 fs, 1 × 10¹⁷ W/cm²) was transported through the channel over a distance of 2 cm, corresponding to 12.5 times the Rayleigh length. © 2001 Scripta Technica, Electr Eng Jpn, 136(3): 19–27, 2001     

Reactive Infiltration of Aluminum into Molybdenum Disilicide Preform

Aluminum has been reactively infiltrated into a molybdenum disilicide preform with relative densities of 40% and 85%. The resultant compact is found to contain Mo(Al,Si)₂ particles surrounded by aluminum metal; 14 to 18 at.% of aluminum reacted with MoSi₂ irrespective of the infiltration temperature and time.     

Determination of unsaturated fatty acid composition by high-resolution nuclear magnetic resonance spectroscopy

High-resolution nuclear magnetic resonance (NMR) spectroscopy provides useful data for analyzing fatty acid compositions of edible vegetable oils. Quantitation of each fatty acid was carried out by evaluation of particular peaks. According to the ¹H NMR method, terminal methyl protons, divinyl protons, and allyl protons are useful to calculate linolenic acid, linoleic acid, and oleic acid, respectively. The ω-2 carbon, divinyl carbon, and allylic carbons were used for calculation of these acids by the ¹³C NMR method. Compositional results obtained by NMR coincided well with those of the conventional gas chromatography (GC) method. Results from ¹³C NMR were in better agreement with those from GC than were the results obtained by the ¹H NMR method.     

The High-Resolution X-Ray Microcalorimeter Spectrometer, SXS, on Astro-H

The science and an overview of the Soft X-ray Spectrometer onboard the STRO-H mission are presented. The SXS consists of X-ray focusing mirrors and a microcalorimeter array and is developed by international collaboration lead by JAXA and NASA with European participation. The detector is a 6×6 format microcalorimeter array operated at a cryogenic temperature of 50 mK and covers a 3′×3′ field of view of the X-ray telescope of 5.6 m focal length. We expect an energy resolution better than 7 eV (FWHM, requirement) with a goal of 4 eV. The effective area of the instrument will be 225 cm² at 7 keV; by a factor of about two larger than that of the X-ray microcalorimeter on board Suzaku. One of the main scientific objectives of the SXS is to investigate turbulent and/or macroscopic motions of hot gas in clusters of galaxies.     

Innovative collagen nano-hydroxyapatite scaffolds offer a highly efficient non-viral gene delivery platform for stem cell-mediated bone formation

The ability of nano-hydroxyapatite (nHA) particles developed in-house to act as non-viral delivery vectors is assessed. These nHA particles are combined with collagen to yield bioactive, biodegradable collagen nano-hydroxyapatite (coll-nHA) scaffolds. Their ability to act as gene-activated matrices for BMP2 delivery is demonstrated with successful transfection of mesenchymal stem cells (MSCs) resulting in high calcium production.     

Utilization of Shochu distillery wastewater for production of polyunsaturated fatty acids and xanthophylls using thraustochytrid

The industrial production of barley shochu, a distilled alcoholic beverage, results in distillery waste that is currently incinerated or disposed of in landfills, causing environmental pollution. The supernatant of distillery waste contains organic matter such as proteins ( approximately 2.5%) and amino acids ( approximately 0.2%). This study demonstrates that the utilization of distillery wastewater as a sole nitrogen source enables a marine thraustochytrid, Schizochytrium sp. strain KH105, to propagate and accumulate valuable lipids including docosahexaenoic acid (DHA) and astaxanthin. Under optimized culture conditions, the highest DHA and astaxanthin yields were obtained at 3.4 g/l and 7.7 mg/l, respectively, after 4 or 5 d of cultivation in a 3-l jar fermentor. The chemical oxygen demand of the wastewater was reduced by 35%. About 67% of crude protein content and 85% of total free amino acid content also decreased in the culture supernatant. The thraustochytrid therefore serves to upgrade the distillery by-product to one containing value-added lipids for functional foods as well as to regulate the environmental contamination.     

The interior coil inference method with the logic parts attaching algorithm for automatic regeneration of sequential control programs

For the replacement of old sequential controllers for plants by modern programmable controllers (PCs), we have developed the sequential control program automatic inductive regeneration (SPAIR) method and system from input–output signals and action specifications of a target controller. However, this method cannot regenerate the logic included in the interior coils. Therefore, we propose an efficient interior coil logic inference method for SPAIR using the Logic Parts Attaching Algorithm. In this method, the target logic of the interior coil is acquired by the selective attachment of logic parts. We confirmed its effectiveness through the application of the method to some original controllers including interior logic. © 2001 Scripta Technica, Electr Eng Jpn, 137(1): 42–47, 2001