Experimental noise effects on transfer function—phase evaluations via Hilbert transforms: Application to optical fibres

The effect of measurement noise on the phase of a linear network-transfer-function, computed as the Hilbert transform of the modulus, is investigated experimentally. As a typical example, the case of an optical fibre is dealt with. It is shown that, when noise is present, theoretically equivalent methods of performing this transform lead to results affected by uncertainties strongly depending on the chosen algorithm.     

Highly efficient full-vectorial 3-D BPM modeling of fiber to planarwaveguide couplers

The behavior of a fiber-slab coupler used as a polarization and wavelength tunable filter is studied both experimentally and theoretically. The theoretical model is based on a three-dimensional (3-D) fully vectorial beam propagation method (BPM), whose main features concern its high efficiency from the point of view of memory and central processing unit (CPU) requirements. The device characteristics have been measured and the results of the comparisons among experimental and theoretical two-dimensional (2-D) and 3-D numerical modeling are reported and discussed. They show a very good agreement In the 3-D case where field polarization, coupling terms and z-variant structures are taken into account     

Asymptotically stable visual servoing of manipulators via neural networks

In this article we present a class of position control schemes for robot manipulators based on feedback of visual information processed through artificial neural networks. We exploit the approximation capabilities of neural networks to avoid the computation of the robot inverse kinematics as well as the inverse task space–camera mapping which involves tedious calibration procedures. Our main stability result establishes rigorously that in spite of the neural network giving an approximation of these mappings, the closed‐loop system including the robot nonlinear dynamics is locally asymptotically stable provided that the Jacobian of the neural network is nonsingular. The feasibility of the proposed neural controller is illustrated through experiments on a planar robot. © 2000 John Wiley & Sons, Inc.     

A reconfigurable and programmable FPGA-based system for nonstandard ultrasound methods

The availability of programmable and reconfigurable ultrasound (US) research platforms may have a considerable impact on the advancement of ultrasound systems technology; indeed, they allow novel transmission strategies or challenging processing methods to be tested and experimentally refined. In this paper, the ULtrasound Advanced Open Platform (ULA-OP), recently developed in our University laboratory, is shown to be a flexible tool that can be easily adapted to a wide range of applications. Five nonstandard working modalities are illustrated. Vector Doppler and quasi-static elastography applications emphasize the real-time potential and versatility of the system. Flow-mediated dilation, pulse compression, and high-frame-rate imaging highlight the flexibility of data access at different points in the reception chain. For each modality, the role played by the onboard programmable devices is discussed. Experimental results are reported, indicating the relative performance of the system for each application.     

Superior Cerebellar Atrophy: An Imaging Clue to Diagnose ITPR1-Related Disorders

The inositol 1,4,5-triphosphate receptor type 1 (ITPR1) gene encodes an InsP₃-gated calcium channel that modulates intracellular Ca²⁺ release and is particularly expressed in cerebellar Purkinje cells. Pathogenic variants in the ITPR1 gene are associated with different types of autosomal dominant spinocerebellar ataxia: SCA15 (adult onset), SCA29 (early-onset), and Gillespie syndrome. Cerebellar atrophy/hypoplasia is invariably detected, but a recognizable neuroradiological pattern has not been identified yet. With the aim of describing ITPR1-related neuroimaging findings, the brain MRI of 14 patients with ITPR1 variants (11 SCA29, 1 SCA15, and 2 Gillespie) were reviewed by expert neuroradiologists. To further evaluate the role of superior vermian and hemispheric cerebellar atrophy as a clue for the diagnosis of ITPR1-related conditions, the ITPR1 gene was sequenced in 5 patients with similar MRI pattern, detecting pathogenic variants in 4 of them. Considering the whole cohort, a distinctive neuroradiological pattern consisting in superior vermian and hemispheric cerebellar atrophy was identified in 83% patients with causative ITPR1 variants, suggesting this MRI finding could represent a hallmark for ITPR1-related disorders.     

Modeling Lung Carcinoids with Zebrafish Tumor Xenograft

Lung carcinoids are neuroendocrine tumors that comprise well-differentiated typical (TCs) and atypical carcinoids (ACs). Preclinical models are indispensable for cancer drug screening since current therapies for advanced carcinoids are not curative. We aimed to develop a novel in vivo model of lung carcinoids based on the xenograft of lung TC (NCI-H835, UMC-11, and NCI-H727) and AC (NCI-H720) cell lines and patient-derived cell cultures in Tg(fli1a:EGFP)^y1 zebrafish embryos. We exploited this platform to test the anti-tumor activity of sulfatinib. The tumorigenic potential of TC and AC implanted cells was evaluated by the quantification of tumor-induced angiogenesis and tumor cell migration as early as 24 h post-injection (hpi). The characterization of tumor-induced angiogenesis was performed in vivo and in real time, coupling the tumor xenograft with selective plane illumination microscopy on implanted zebrafish embryos. TC-implanted cells displayed a higher pro-angiogenic potential compared to AC cells, which inversely showed a relevant migratory behavior within 48 hpi. Sulfatinib inhibited tumor-induced angiogenesis, without affecting tumor cell spread in both TC and AC implanted embryos. In conclusion, zebrafish embryos implanted with TC and AC cells faithfully recapitulate the tumor behavior of human lung carcinoids and appear to be a promising platform for drug screening.     

Fast silicon p doped low temperature bolometer

The construction of a fast silicon p doped low temperature bolometer is described. It is a 5 × 5 × 0.3 mm Si <100 > n-type chip whose surface has been implanted with p doses of the order of 10¹⁸cm⁻³. The bolometer has a response time better than 1 μs, a responsivity of 10⁴VW⁻¹ and a NEP of .     

Ionic interactions and the global conformations of the hammerhead ribozyme

Here we investigate the global conformation of the hammerhead ribozyme. Electrophoretic studies demonstrate that the structure is folded in response to the concentration and type of ions present. Folding based on colinear alignment of arms II and III is suggested, with a variable angle subtended by the remaining helix I. In the probable active conformation, a small angle is subtended between helices I and II. Using uranyl photocleavage, an ion binding site has been detected in the long single-stranded region. The folded conformation could generate a preactivation of the scissile bond to permit in-line attack of the 2'-hydroxyl group, with a bound metal ion playing an integral role in the chemistry.