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排序方式: 共有649条查询结果,搜索用时 15 毫秒
101.
Richard G. Sherman Michael Gay Willem van Ast Kenneth S. Chin 《Tunnelling and Underground Space Technology incorporating Trenchless Technology Research》1994,9(3)
In February 1989, the Massachusetts Water Resource Authority (MWRA) began the design and construction of a 15.3-km subsea tunnel and ocean riser system to discharge effluent in conjunction with the design and construction of a large secondary treatment plant. The court-mandated schedule required the tunnel and risers to be completed in July 1995. The paper discusses the project from the viewpoints of management, concept design and construction methods, including the ocean riser system. Key to th success of the project is the accuracy of the location of the risers. The design calls for a final tunnel alignment to be driven within 10 m of the riser shafts, beginning at a distance of 13.1 km from shore. 相似文献
102.
Schalk Kok Nielen Stander Willem Roux 《International journal for numerical methods in engineering》1998,43(1):1-21
Response surface methodology is used to construct approximations to temperature and stress in transient thermoelastic analysis of non-linear systems. The analysis forms the core component of a heating/cooling rate maximization problem in which the ordinates of the ambient temperature at equally spaced time intervals are chosen as the design variables. Polynomials or cubic splines are fitted through the ordinates to describe the ambient temperature profile required for the convective heat transfer analysis. An experimental design method based on D-optimality and a genetic algorithm was used to select the design points used to create the approximations. Linear response surfaces were found to be sufficiently accurate, thereby minimizing the number of finite element analyses. Two examples of which one is a thick-walled pressure vessel are used to illustrate the methodology. © 1998 John Wiley & Sons, Ltd. 相似文献
103.
Pierre Van Rensburg Willem H. Van Zyl Isak S. Pretorius 《Yeast (Chichester, England)》1998,14(1):67-76
Saccharomyces cerevisiae produces several β-1,3-glucanases, but lacks the multicomponent cellulase complexes that hydrolyse the β-1,4-linked glucose polymers present in cellulose-rich biomass as well as in haze-forming glucans in certain wines and beers. We have introduced into S. cerevisiae a functional cellulase complex for efficient cellulose degradation by cloning the Endomyces fibuliger cellobiase (BGL1) gene and co-expressing it with the Butyrivibrio fibrisolvens endo-β-1,4-glucanase (END1), the Phanerochaete chrysosporium cellobiohydrolase (CBH1) and the Ruminococcus flavefaciens cellodextrinase (CEL1) gene constructs in this yeast. The END1, CBH1 and CEL1 genes were inserted into yeast expression/secretion cassettes. Expression of END1, CBH1 and CEL1 was directed by the promoter sequences derived from the alcohol dehydrogenase II (ADH2), the phosphoglycerate kinase I (PKG1) and the alcohol dehydrogenase I (ADH1) genes, respectively. In contrast, BGL1 was expressed under the control of its native promoter. Secretion of End1p and Cel1p was directed by the signal sequence of the yeast mating pheromone α-factor (MFα1), whereas Cbh1p and Bgl1p were secreted using their authentic leader peptides. The construction of a fur1 ura3 S. cerevisiae strain allowed for the autoselection of this multicopy URA3-based plasmid in rich medium. S. cerevisiae transformants secreting biologically active endo-β-1,4-glucanase, cellobiohydrolase, cellodextrinase and cellobiase were able to degrade various substrates including carboxymethylcellulose, hydroxyethylcellulose, laminarin, barley glucan, cellobiose, polypectate, birchwood xylan and methyl-β-d-glucopyranoside. This study could lead to the development of industrial strains of S. cerevisiae capable of converting cellulose in a one-step process into commercially important commodities. © 1998 John Wiley & Sons, Ltd. 相似文献
104.
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106.
de Jong Peter F.; Koomen Willem; Mellenbergh Gideon J. 《Canadian Metallurgical Quarterly》1988,55(5):718
Investigated whether B. Weiner's (1979) model of causal attributions applies to perceptions of the causes for success and failure. Instead of the usual similarity judgments, preference judgments were used to reveal the dimensions underlying these perceptions. Female subjects, randomly assigned to a success or failure condition, made preference judgments with regard to 12 causes for success or failure. Multidimensional scaling analysis uncovered internality, stability, and excusability dimensions, thus supporting Weiner's model at least partly. In addition, differences in the relative emphasis given to the dimensions were found between the success and failure conditions: Following success, the internality dimension was the most salient, whereas after failure the stability and excusability dimensions were the most important. Furthermore, after success subjects preferred more internal causes and more causes that seem inappropriate as an excuse. After failure, subjects chose more external and more excusable causes. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
107.
108.
Measuring free metal ion concentrations in situ in natural waters using the Donnan Membrane Technique 总被引:1,自引:0,他引:1
Kalis EJ Weng L Dousma F Temminghoff EJ Van Riemsdijk WH 《Environmental science & technology》2006,40(3):955-961
Metal toxicity is not related to the total but rather to the free or labile metal ion concentration. One of the techniques that can be used to measure several free metal ion concentrations simultaneously is the Donnan Membrane Technique (DMT) in combination with the inductively coupled plasma-mass spectrometer (ICP-MS). However, free metal ion concentrations in natural waters are commonly below the detection limit of ICP-MS. We decreased the detection limit by making use of a ligand, and we developed a field DMT cell that can be applied in situ in natural waters. A kinetic approach can be used to calculate free metal ion concentrations when the equilibrium time becomes too large. The field DMT measured in situ in natural waters a free metal ion concentration ranging from 0.015% (Cu) to 13% (Zn) of a total metal concentration ranging from 0.06 nM (Cd) to 237 nM (Zn). The free metal ion concentrations were difficult to predict using an equilibrium speciation model, probably due to the uncertainty in the nature of the dissolved organic matter or the presence of other reactive colloids. It is shown that DMT can follow changes in the free metal ion concentration on times scales less than a day under certain conditions. 相似文献
109.
Mulder WJ Koole R Brandwijk RJ Storm G Chin PT Strijkers GJ de Mello Donegá C Nicolay K Griffioen AW 《Nano letters》2006,6(1):1-6
MRI detectable and targeted quantum dots were developed. To that aim, quantum dots were coated with paramagnetic and pegylated lipids, which resulted in a relaxivity, r(1), of nearly 2000 mM(-1)s(-1) per quantum dot. The quantum dots were functionalized by covalently linking alphavbeta3-specific RGD peptides, and the specificity was assessed and confirmed on cultured endothelial cells. The bimodal character, the high relaxivity, and the specificity of this nanoparticulate probe make it an excellent contrast agent for molecular imaging purposes. 相似文献
110.
For the analysis of thyroid transporter ligands, a triple bioaffinity mass spectrometry (BioMS) concept was developed, with the aim at three different analytical objectives: rapid screening of any ligand, confirmation of known ligands in accordance with legislative requirements, and identification of emerging yet unknown ligands. These three purposes share the same biorecognition element, recombinant thyroid transport protein transthyretin (rTTR), and dedicated modes of liquid chromatography-mass spectrometry (LC-MS). For screening, a rapid and radiolabel-free competitive inhibition MS binding assay was developed with fast ultrahigh performance-liquid chromatography-electrospray ionization-triple-quadrupole-MS (UPLC-QqQ-MS) as the readout system. It uses the nonradioactive stable isotopic thyroid hormone (13)C(6)-l-thyroxine as the label of which the binding to rTTR is inhibited by any ligand such as thyroid drugs and thyroid endocrine disrupting chemicals (EDCs). To this end, rTTR is either used in solution or immobilized on paramagnetic microbeads. The concentration-dependent inhibition of the label by the natural thyroid hormone l-thyroxine (T4), as a model analyte, is demonstrated in water at part-per-trillion and in urine at part-per-billion level. For confirmation of identity of known ligands, rTTR was used for bioaffinity purification for confirmation of naturally present free T4 in urine. As a demonstrator for identification of unknown ligands, the same rTTR was used again but in combination with nano-UPLC-quadrupole time-of-flight-MS (nLC-Q-TOF-MS) and urine samples spiked with the model "unknown" EDCs triclosan and tetrabromobisphenol-A. This study highlights the potential of BioMS using one affinity system, both for rapid screening and for confirmation and identification of known and unknown emerging thyroid EDCs. 相似文献