Influence of different fat emulsions with 10 or 20% MCT/LCT or LCT on lipoproteins in plasma of patients after abdominal surgery

In patients after elective abdominal surgery, different fat emulsions were used to compare their efficacy in total parenteral nutrition and in normalizing plasma lipoprotein levels. In five different groups with 5 patients each, half of the nonprotein calories were given as medium-chain triglycerides/long-chain triglycerides (1:1) or as long-chain triglycerides alone in 10 or 20% fat emulsions or as glucose alone in a control group for 7 days. After surgery, an initial decrease of all plasma lipoprotein components was followed by a different behavior of glyceride-glycerol, cholesterol, phospholipids, and apolipoproteins. Glyceride-glycerol in very-low-density lipoproteins and high-density lipoproteins is increasing during infusion of fat emulsions and decreasing during overnight interruption of infusions. After the 7-day infusion period, there was no significant difference in very-low-density lipoprotein glyceride-glycerol as compared with the values before different infusions. Low-density lipoprotein cholesterol is reaching and exceeding preoperative concentrations between the 4th and the 7th day, most during infusion of 10% fat emulsion and especially due to an increase of free cholesterol. High-density lipoprotein cholesterol and apolipoprotein A-I reach preoperative levels during infusion of fat emulsions but not with glucose alone. Higher than preoperative values are reached in phospholipids with all fat infusions already on day 4. Abnormal lipoprotein X occurred least with the medium-chain/long-chain triglyceride 20% fat-infusion. This fat emulsion is suggested as having the best normalizing effect on plasma lipoproteins and best tolerance in patients after surgery.     

In situ antigen immobilization for stable organic-phase immunoelectrodes

A new method based on enzymatic single-step in situ synthesis of hapten-carrier conjugates on electrodes is described yielding stable, reproducible, and reusable organic-phase immunoelectrodes (OPIEs). The electrodes developed were tailored for analyte detection in organic solvents and allow for the analysis of soil extracts without further sample processing and cleanup. Catalyzed by transglutaminase from a variant of Streptoverticillium mobaraense, the reaction proceeds in aqueous solution with and without addition of organic media in only 1.5 hours. In this study, the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) was chosen as model compound and chemically amino-functionalized prior to its enzymatic immobilization. The high reproducibility of the immobilization procedure allowed for batch calibration of the immunoelectrodes. Moreover, pure methanol or treatment with diluted sulfuric acid used for regeneration studies did not disturb the hapten layer. The OPIE consists of screen-printed carbon electrodes, monoclonal anti-2,4-D antibodies, and the immunochemical recognition reaction and was optimized with regard to a high stability in organic media. For electrochemical detection, horseradish peroxidase was used as enzyme label together with H2O2 as substrate and hexacyanoferrate (II)/(III) as mediator. The OPIE showed high stability upon storage over 93 days. Response times of 17 s (t95) were found to be advantageous compared to those of other biosensors. Including the immunochemical reactions, the complete assay takes 30 min. A calibration curve for 2,4-D in 30% methanol/buffer obtained with 70 electrodes within 4 weeks revealed a detection limit of 9 mg/L, a sensitivity of 1.3 nA L mg-1 cm-2, and a repeatability of 6.8%. Although we calculated a lowered repeatability for reused electrodes of 13.4% and a slightly decreased sensitivity of 0.9 nA L mg-1 cm-2, multiple-used OPIEs could also be applied for calibration.     

Numerical dissipation and the bottleneck effect in simulations of compressible isotropic turbulence

The piece-wise parabolic method (PPM) is applied to simulations of forced isotropic turbulence with Mach numbers ∼0.1 … 1. The equation of state is dominated by the Fermi pressure of an electron-degenerate fluid. The dissipation in these simulations is of purely numerical origin. For the dimensionless mean rate of dissipation, we find values in agreement with known results from mostly incompressible turbulence simulations. The calculation of a Smagorinsky length corresponding to the rate of numerical dissipation supports the notion of the PPM supplying an implicit subgrid scale model. In the turbulence energy spectra of various flow realisations, we find the so-called bottleneck phenomenon, i.e., a flattening of the spectrum function near the wave number of maximal dissipation. The shape of the bottleneck peak in the compensated spectrum functions is comparable to what is found in turbulence simulations with hyperviscosity. Although the bottleneck effect reduces the range of nearly inertial length scales considerably, we are able to estimate the value of the Kolmogorov constant. For steady turbulence with a balance between energy injection and dissipation, it appears that C ≈ 1.7. However, a smaller value is found in the case of transonic turbulence with a large fraction of compressive components in the driving force. Moreover, we discuss length scales related to the dissipation, in particular, an effective numerical length scale Δ_eff, which can be regarded as the characteristic smoothing length of the implicit filter associated with the PPM.     

Buchbesprechungen

Ohne Zusammenfassung