Modulation of cytokine release and neutrophil function by granulocyte colony-stimulating factor during endotoxemia in humans

In this double-blind, cross-over, placebo-controlled, randomized study, two groups of eight healthy male volunteers were challenged with endotoxin (4 ng/kg) on two occasions, once in conjunction with placebo and once with granulocyte colony-stimulating factor (G-CSF; 5 microg/kg). In group 1, G-CSF was administered intravenously 2 hours before endotoxin challenge; in group 2, G-CSF was administered subcutaneously 24 hours before endotoxin challenge. In group 1, G-CSF significantly enhanced the release of tumor necrosis factor (TNF), interleukin-6 (IL-6), IL-8, IL-1 receptor antagonist (IL-1ra), and soluble TNF receptors. In group 2, G-CSF significantly reduced IL-8 concentrations and modestly attenuated TNF and IL-6 levels. In this group, IL-1ra and soluble TNF receptors were enhanced by G-CSF pretreatment and lipopolysaccharide (LPS)-induced soluble TNF receptor release was further augmented, whereas LPS-induced IL-1ra concentrations remained unaltered. Both pretreatments with G-CSF increased LPS-induced peripheral neutrophilia; the expression of CD11b, CD18, and CD67; and the release of elastase and lactoferrin. Both pretreatments also down-regulated neutrophil L-selectin expression and prevented the endotoxin-induced pulmonary neutrophil accumulation during the first 2 hours after endotoxin challenge. These data indicate that two different pretreatments with G-CSF result in differential effects on LPS-induced cytokine release but similar effects on LPS-induced neutrophil activation and changes in expression of cell surface molecules. Finally, regardless of the effects of G-CSF on LPS-induced cytokine release, G-CSF blocks LPS-induced pulmonary granulocyte accumulation.     

Measurement of neutron excitation functions using wide energy neutron beams

A technique for measuring neutron excitation functions using wide energy neutron beams is explored. Samples are activated with a set of neutron fields, each covering a relatively wide energy interval and created using an ion accelerator and conventional nuclear reactions. Measured activities are determined using gamma-ray spectrometry and reduced to excitation curves using spectrum unfolding. The technique is demonstrated on the measurement of the excitation function curve up to 5.6 MeV for ¹¹³In(n,n′)¹¹³In^m using the ¹¹⁵In(n,n′)¹¹⁵In^m reaction as an internal standard.     

Design notations for secure software: a systematic literature review

In the past 10 years, the research community has produced a significant number of design notations to represent security properties and concepts in a design artifact. These notations are aimed at documenting and analyzing security in a software design model. The fragmentation of the research space, however, has resulted in a complex tangle of different techniques. Hence, practitioners are confronted with the challenging task of scouting the right approach from a multitude of proposals. Similarly, it is hard for researchers to keep track of the synergies among the existing notations, in order to identify the existing opportunities for original contributions. This paper presents a systematic literature review that inventorizes the existing notations and provides an in-depth, comparative analysis for each.     

The City Trip Planner: An expert system for tourists

Over the last few years, advanced digital applications have become available to tourists. Some of these offer the possibility of creating personalised routes. This paper introduces a tourist expert system, called the City Trip Planner, that allows planning routes for five cities in Belgium. It is implemented as a web application that takes into account the interests and trip constraints of the user and matches these to a database of locations in order to predict personal interests. A fast and effective planning algorithm provides an on-the-fly suggestion of a personal trip for a requested number of days, taking into account opening hours of attractions and time for a (lunch) break. The expert system is discussed in detail. Usage statistics and user feedback demonstrate that it is highly appreciated by tourists.