Waveguide stacks of GeSiO2-based glass deposited on Si exhibit a permanent second-order nonlinearity following temperature electric-field poling. High temperature hydrogen loading increases the second harmonic signal under certain conditions 相似文献
Temperature-regulated expression of recombinant proteins in the tac promoter (Ptac) system was investigated. Expression levels of fungal xylanase and cellulase from N. patriciarum in E. coli strains containing the natural lacI gene under the control of the Ptac markedly increased with increasing cultivation temperature in the absence of a chemical inducer. The specific activities (units per milligram protein of crude enzyme) of the fungal xylanase and cellulase produced from recombinant E. coli strain pop2136 grown at 42 degrees C were about 4.5 times higher than those of the cells grown at 23 degrees C and were even slightly higher when compared with cells grown in the presence of the inducer isopropyl beta-D-thiogalactopyranoside. The xylanase expression level in the temperature-regulated Ptac system was about 35% of total cellular protein. However, this system can not be applied to E. coli strains containing lacIq, which confers over production of the lac repressor, for high-level expression of recombinant proteins. In comparison with the lambda PL system, the Ptac-based xylanase plasmid in E. coli pop2136 gave a considerably higher specific activity of the xylanase than did the best lambda PL-based construct using the same thermal induction procedure. The high-level expression of the xylanase using the temperature-regulated Ptac system was also obtained in 10-litre fermentation studies using a fed-batch process. These results unambiguously demonstrated that the temperature-modulated Ptac system can be used for overproduction of some non-toxic recombinant proteins. 相似文献
Rapid prototyping has imposed new requirements on the processing of CAD models. To manufacture an object directly, the model must correspond to a real object and thus represent a closed volume. Its boundary must be continuous and closed, and no cracks or improper intersections are allowed. CAD models cannot always meet these requirements, especially when they are constructed by a surface modeler. The algorithm discussed analyzes surface models and generates a topological structure for them that meets rapid prototyping requirements for a closed volume with a continuous, closed boundary 相似文献
An analysis is given of the performance of the standard forgetting factor recursive least squares (RLS) algorithm when used for tracking time-varying linear regression models. Three basic results are obtained: (1) the ‘P-matrix’ in the algorithm remains bounded if and only if the (time-varying) covariance matrix of the regressors is uniformly non-singular; (2) if so, the parameter tracking error covariance matrix is of the order O(μ + γ2/μ), where μ = 1 - λ, λ is the forgetting factor and γ is a quantity reflecting the speed of the parameter variations; (3) this covariance matrix can be arbitrarily well approximated (for small enough μ) by an expression that is easy to compute. 相似文献
Disturbances of signals on a coplanar waveguide (CPW) induced by the presence of LiTaO/sub 3/ and GaAs electrooptic probes in external electrooptic (EO) sampling have been simulated and compared quantitatively. The finite-difference-time-domain method is used to simulate the full wave field around a coplanar waveguide on a GaAs substrate in an external EO sampling configuration. The results indicate that the induced signal disturbance, or invasiveness, of a LiTaO/sub 3/ probe is almost ten times that of a GaAs probe in terms of the magnitude of S/sub 11/, but that LiTaO/sub 3/ yields about two times the EO response for a given S/sub 11/ and optical probing wavelength. The transparency of LiTaO/sub 3/ to shorter wavelengths, however, allows an even higher sensitivity for this material relative to GaAs. The results suggest that these probes do not exhibit significant invasiveness (magnitude of S/sub 11/ smaller than -40 dB), if they are removed from contact by the distance of CPWs center conductor width.<> 相似文献
针对移动无线传感器网络设计一种不依赖于节点地理位置的基于移动汇聚节点(Sink)的数据收集算法(Mobile Sink-based Data Gathering,MSDG)。该算法解决了无线传感器网络中多跳路由通信时出现能量空洞的"热点"问题。Sink沿途以最近的固定节点作为根节点动态构建路由树。簇内移动节点感知的数据经簇头进行数据融合计算,然后将融合后的数据沿路由树反向逐跳转发给Sink。仿真结果表明,MSDG在节点的平均能耗和网络生存时间等方面的性能远超过LEACH、ACE-L等数据收集协议。 相似文献
Silver/superconductor composites containing 0 to 80 vol% silver have been prepared and their properties determined. Optimum heat treatment at sintering temperatures ( 800° C) under low oxygen pressures produces material with high critical current density and improves physical properties. Magnetic susceptibility measurements have been found to be consistent with resistivity results. In order to retain a single high-Tc phase with increasing silver content, decreased oxygen partial pressures are required. Using the normal-state resistivity of these composites, a percolation threshold at a silver volume fraction of 43% was observed, while zero resistivity measurements show that a continuous superconducting network can be obtained with up to 80 vol% silver. The critical current density of 21 vol% silver-doped samples was found to be 1520 A cm–2 at 77.3 K, compared to 260 A cm–2 for an undoped sample. 相似文献
In the context of human-robot and robot-robot interactions, the better cooperation can be achieved by predicting the other party’s subsequent actions based on the current action of the other party. The time duration for adjustment is not sufficient provided by short term forecasting models to robots. A longer duration can by achieved by mid-term forecasting. But the mid-term forecasting models introduce the previous errors into the follow-up forecasting and amplified gradually, eventually invalidating the forecasting. A new mid-term forecasting with error suppression based on restricted Boltzmann machine(RBM) is proposed in this paper. The proposed model can suppress the error amplification by replacing the previous inputs with their features, which are retrieved by a deep belief network(DBN). Furthermore, a new mechanism is proposed to decide whether the forecasting result is accepted or not. The model is evaluated with several datasets. The reported experiments demonstrate the superior performance of the proposed model compared to the state-of-the-art approaches.