用于腺苷检测的基于级联扩增核酸适体电化学传感界面的构建研究

该文报道了一种基于酶级联扩增的电化学核酸适体检测腺苷的新方法。当腺苷存在时,滚环扩增的引物通过E.coli DNA连接酶的作用与通过巯基组装在金电极上的固定探针连接。在pHi29 DNA聚合酶的作用下,滚环扩增反应进行并产生一条与环形探针完全互补的长的单链,然后将大量金纳米粒子标记的核酸探针与滚环扩增的产物杂交。该传感界面电化学行为的结果表明,通过酶级联扩增的方法提高了检测腺苷的阻抗响应灵敏度,且选择性和重现性良好。用于腺苷的检测时,其线性范围为2.0μmol/L~100μmol/L,最低检测浓度为2.0μmol/L,表明该传感界面的设计可作为一种通用方法而有望用于其它目标物的检测。     

独立学院VFP程序设计教学存在的问题及解决方法

根据独立学院的学生特征和VFP课程的教学现状,以提高学生的计算机应用能力为目的,提出摒弃传统教学"重理论,轻实践"的思想,采用案例教学法和任务驱动法,在真实情境中让学生自己动手由浅入深,逐步开发出一个完整的管理信息系统.     

浅析电子商务中的安全问题

随着现今社会互联网、个人终端技术的飞越发展,电子商务越来越多的出现在人们的生活与工作中,这种全新的商务模式具有便捷、高效率、低成本的特点.同时,这种全新的商务模式对管理水平、信息传递技术都提出了更高的要求,其中安全的重要性尤为突出.     

计算机网络安全及防范策略探究

随着计算机科学技术的发展,计算机网络已广泛用于经济、军事、教育等各个领域。因此,计算机的网络安全便成为涉及个人、单位、社会、国家信息安全的重大问题。如何保障网络信息的安全已成为当前人们迫在眉睫需要解决的问题。本文就计算机网络安全技术及相关策略提出了一点粗浅的看法。     

电力监控系统的跨平台解决方案

随着应用的发展,电力监控系统越来越大型化,跨平台的应用越来越多.本文阐述了采用分层归纳的设计思想,归纳电力系统数据处理的逻辑应用层,包装与具体操作系统密切相关的操作系统层,提炼与具体操作系统进程机制有关的程序框架,来实现电力监控软件系统的跨平台应用.并以C语言为主要编程语言进行了电力监控系统跨平台实现,结果表明该方法能够跨越Windows、Linux、Solaris等平台,达到源代码级的跨平台效果.     

Lightweight deep learning methods for panoramic dental X-ray image segmentation

Neural Computing and Applications - Dental X-ray image segmentation is helpful for assisting clinicians to examine tooth conditions and identify dental diseases. Fast and lightweight segmentation...     

Avoiding negative elastic moduli when using Lagrange interpolation for material grading in finite element analysis

Polynomial interpolations, one of the most common interpolations used in finite element methods (FEMs), are a workhorse of many FEM codes. These interpolations are readily available for all kinds of elements, and using them for modeling the variation of elastic moduli in graded elements is thus both convenient and natural. Yet, like all polynomial interpolations, they can be prone to oscillations that can result in regions of negative elastic modulus in the element, even with only positive nodal values of elastic moduli. The result of these negative modulus regions, even if the region is small, can be unexpected singularities in the solution. This defeats the purpose of using polynomial interpolations for capturing material grading in the element. We demonstrate the issue using three-node quadratic Lagrange interpolations of material grading in otherwise isoparametric p-type elements and show how to avoid this problem.     

Array SSL VPN如何保障番禺有线办公网的安全运行

企业办公自动化系统的应用十分广泛,随着互联网的高速发展,WEB应用成为主流,OA系统在互联网上的延伸成为大趋势,本文阐述在WEB应用中如何保障数据和系统安全的手段和方法。     

Structures and spectroscopic properties of nonperipherally and peripherally substituted metal-free phthalocyanines: a substitution effect study based on density functional theory calculations

The molecular structures, molecular orbitals, atomic charges, electronic absorption spectra, and infrared (IR) and Raman spectra of a series of substituted metal-free phthalocyanine compounds with four (1, 3, 5, 7) or eight (2, 4, 6, 8) methoxyl (1, 2, 5, 6) or methylthio groups (3, 4, 7, 8) on the nonperipheral (1-4) or peripheral positions (5-8) of the phthalocyanine ring are studied by density functional theory (DFT) and time-dependent DFT (TD-DFT) calculations. The calculated structural parameters and simulated electronic absorption and IR spectra are compared with the X-ray crystallography structures and the experimentally observed electronic absorption and IR spectra of the similar molecules, and good agreement between the calculated and experimental results is found. The substitution of the methoxyl or methylthio groups at the nonperipheral positions of the phthalocyanine ring has obvious effects on the molecular structure and spectroscopic properties of the metal-free phthalocyanine. Nonperipheral substitution has a more significant influence than peripheral substitution. The substitution effect increases with an increase in the number of substituents. The methylthio group shows more significant influence than the methoxyl group, despite the stronger electron-donating property of the methoxyl group than the methylthio group. The octa-methylthio-substituted metal-free phthalocyanine compounds have nonplanar structures whose low-lying occupied molecular orbitals and electronic absorption spectra are significantly changed by the substituents. The present systematical study will be helpful for understanding the relationship between structures and properties in phthalocyanine compounds and designing phthalocyanines with typical properties.