Solution conformation of the Pseudomonas syringae MSU 16H phytotoxic lipodepsipeptide Pseudomycin A determined by computer simulations using distance geometry and molecular dynamics from NMR data

Pseudomycin A is a cyclic lipodepsinonapeptide phytotoxin produced by a strain of the plant pathogenic bacterium Pseudomonas syringae. Like other members of this family of bacterial metabolites, it is characterised by a fatty acylated cyclic peptide with mixed chirality and lactonic closure. Several biological activities of Pseudomycin A are lower than those found for some of its congeners, a difference which might depend on the diverse number and distribution of charged residues in the peptide moiety. Hence, it was of interest to investigate its conformation in solution. After the complete interpretation of the two-dimensional NMR spectra, NOE data were obtained and the structure was determined by computer simulations, applying distance geometry and molecular dynamics procedures. The conformation of the large ring of Pseudomycin A in solution includes three rigid structural regions interrupted by three short flexible regions that act as hinges. The overall three-dimensional structure of the cyclic moiety is similar to that of previously studied bioactive lipodepsinonapeptides produced by other pseudomonads.     

In vitro short-term response of human erythrocytes to implant materials used in maxillo-facial rigid internal fixation

A number of retinal proteins are phosphorylated by a variety of kinases, resulting in well-known regulatory effects. The identity and role of corresponding phosphatases is less clear. We simultaneously measured the activity of serine/ threonine protein phosphatases type 1, 2A and 2C in bovine retinae. The enzymes were classified according to substrate specificity, divalent cation requirement and the effect of phosphatase subtype-specific inhibitors. The total- and specific activity of phosphatase type 2A was prevalent. Type 2C was 10-fold less abundant. 80% of type 1 and 50% of type 2A and type 2C, respectively, were soluble. An economic purification scheme was developed. We demonstrated the presence of phosphatase isozymes 2Calpha and 2Cbeta in bovine rod outer segments by enzymatic analysis as well as immunological techniques. The results suggest a yet unknown role of phosphatase type 2C in phototransduction. On the other hand, the immense amount of protein phosphatases found to be soluble - therefore not associated with rod outer segment membranes - points towards participation of these enzymes in the process of visual transduction not considered thus far.     

长输管道建设物流管理新模式——物资配送

长输管道工程是以线路管道与沿途站场为主要内容的建设工程,其物资配送有着不同于一般物流管理的特点,点多线长,且交通不便.文章结合西南成品油管道建设工程的物资配送实践,总结出长输管道物资配送的类型,并从机构设置、计划编制、台账和报表管理等方面介绍了物资配送的管理内容及经验,实践证明,这种物资配送方法不但使业主的物资管理人员大为减少,而且使长输管道工程的物资供应更加及时有序.     

日本年轻建筑师的现状——1960年代的这一群

一人脉对照 1 日本现代建筑师溯流 引领日本现代建筑进人成熟时期的领袖人物非丹下健三莫属。由师从丹下的模文彦（1928年-）、黑川纪章（1934年-）以及大高正人（1923年-）、菊竹清训（1928年-）等4人在1960年代创导的新陈代谢运动，使主流的欧美建筑界开始认真关注一个亚洲的小国——日本的建筑活动。可以说新陈代谢运动是日本现代建筑发展的基石也是随后其建筑发展的原动力。     

下消化道出血DSA诊断及栓塞结合小剂量垂体后叶素灌注治疗

目的探讨不明原因下消化道出血的DSA诊断及介入栓塞治疗的临床应用价值.方法对32例不明原因下消化道出血患者行DSA检查,通过显示造影剂外溢、病理血管和肿瘤染色来确定出血原因及部位,对出血处行超选择性动脉栓塞治疗和留置导管灌注小剂量垂体后叶素维持12 h止血治疗.结果DSA诊断阳性率75%(24/32),其中肠伤寒2例,肠结核1例,血管性病变14例,肿瘤7例.阳性者介入手术成功20例,手术成功率83.3%,无一例严重并发症.结论DSA检查和介入治疗对诊断不明确或保守治疗无效的下消化道出血有着重要的诊断及治疗     

NCP101x系列转换开关在超高亮度Luxeon Star LED驱动电路中的应用

NCP101x系列自供电转换开关集成了固定频率电流模式控制器和900 V的MOSFET.介绍NCP101x系列电路的引脚功能和特点,给出基于NCP101x的Luxeon Star发光二极管(LED)驱动电路.     

新疆重质混合原油研制高等级道路沥青

对利用新疆重质混合原油生产的减压渣油作为基质沥青制备高等级道路沥青进行了研究。考察了低硫石蜡基原油（A）和含硫环烷-中间基原油（B）的掺炼比对基质沥青四组分组成的影响，减压渣油软化点与基质沥青四组分组成的关系，以及添加剂对改进沥青性质的作用。由此得出原油（B）的合适掺炼量，减压渣油软化点的控制范围和优化的生产工艺条件，调合出了符合GB／T18150—2000和JTGF40—2004要求的高等级道路沥青。     

中频反应溅射SiO2膜与直流溅射ITO膜的在线联镀

多数ITO透明导电玻璃生产线在实现SiO2膜与ITO膜在线联镀时，应用SiO2靶射频溅射沉积SiO2膜工艺和ITO靶直流溅射沉积ITO膜工艺，如果SiO2膜应用硅靶反应磁控溅射工艺，存在这种工艺是否可以与ITO靶直流溅射沉积ITO膜工艺在线联用以及如何实现联用的问题。作者对现有的生产线进行了改造设计、加工，做了大量实验、质谱分析和多项测试研究，成功地实现反应溅射SiO2膜与ITO膜在线联镀，做到SiO2镀膜室的工作状态的变化基本上不影响ITO镀膜室的工艺条件。     

根本技术创新产品需求的获取及评价研究

根本技术创新对企业的发展具有决定性的意义。并对工程作为现代产品开发中新发展的系统化方法，在应用其对根本技术创新产品需求搜集及评价时，出现了满足顾客需求和创造顾客需求、资源需求与技术创新、理性决策与非理性决策、企业文化与技术创新等之间的矛盾。本文对产生这些矛盾的原因进行了分析，提出了相应的解决办法。     

关于最远点判定及直径的唯一性

给出了点到有界集最远点的一些必要或充分条件，然后对直径的唯一性也给出了一些判定条件。