用于腺苷检测的基于级联扩增核酸适体电化学传感界面的构建研究

该文报道了一种基于酶级联扩增的电化学核酸适体检测腺苷的新方法。当腺苷存在时,滚环扩增的引物通过E.coli DNA连接酶的作用与通过巯基组装在金电极上的固定探针连接。在pHi29 DNA聚合酶的作用下,滚环扩增反应进行并产生一条与环形探针完全互补的长的单链,然后将大量金纳米粒子标记的核酸探针与滚环扩增的产物杂交。该传感界面电化学行为的结果表明,通过酶级联扩增的方法提高了检测腺苷的阻抗响应灵敏度,且选择性和重现性良好。用于腺苷的检测时,其线性范围为2.0μmol/L~100μmol/L,最低检测浓度为2.0μmol/L,表明该传感界面的设计可作为一种通用方法而有望用于其它目标物的检测。     

Treatment of linear alkylbenzene sulfonate (LAS) wastewater by internal electrolysis--biological contact oxidation process

Surfactant wastewater is usually difficult to treat due to its toxicity and poor biodegradability. A separate physico-chemical or biochemical treatment method achieves a satisfactory effect with difficulty. In this study, treatment of the wastewater collected from a daily chemical plant by the combination processes of Fe/C internal electrolysis and biological contact oxidation was investigated. For the internal electrolysis process, the optimal conditions were: pH = 4-5, Fe/C = (10-15):1, air-water ratio = (10-20):1 and hydraulic retention time (HRT)= 2 h. For the biological contact oxidation process, the optimal conditions were: HRT = 12 h, DO = 4.0-5.0 mg/L. Treated by the above combined processes, the effluent could meet the I-grade criteria specified in Integrated Wastewater Discharge Standard of China (GB 8978-1996). The results provide valuable information for full-scale linear alkylbenzene sulfonate wastewater treatment.     

A new image fusion performance metric based on visual information fidelity

Because subjective evaluation is not adequate for assessing work in an automatic system, using an objective image fusion performance metric is a common approach to evaluate the quality of different fusion schemes. In this paper, a multi-resolution image fusion metric using visual information fidelity (VIF) is presented to assess fusion performance objectively. This method has four stages: (1) Source and fused images are filtered and divided into blocks. (2) Visual information is evaluated with and without distortion information in each block. (3) The visual information fidelity for fusion (VIFF) of each sub-band is calculated. (4) The overall quality measure is determined by weighting the VIFF of each sub-band. In our experiment, the proposed fusion assessment method is compared with several existing fusion metrics using the subjective test dataset provided by Petrovic. We found that VIFF performs better in terms of both human perception matching and computational complexity.     

交互式白板在教学中的应用

交互式白板实现了与计算机的交互控制,是一种集软硬件技术于一体的媒体技术。不同于其他媒体,其对教学的影响是多方面的,内容的呈现、互动环境的营造、课堂的生成、教学设计等等。随着交互式白板在教学中的应用,广大师生将对其进行更加深入的研究。     

光学薄膜元器件外观特性机器视觉检测系统

本系统利用工业级摄像头及开发包的机器视觉功能与计算软件编程控制技术相结合,通过与输入控制系统中的标准膜片模板外观品质进行对比,实现高速高效、准确识别检测光学薄膜器件中滤光片的外观缺陷。可在生产线上根据外观品质标准模板中设定的类别进行自动分类装盒。     

安钢SAP预留系统的开发与应用

本文简述了安钢信息化建设概况,介绍了安钢信息化建设中开发SAP预留系统的设计流程,阐述了预留系统实现的功能.     

采用动态邻域扩展算法的非一致CA低功耗确定TPG

针对确定内建自测试向量发生器设计中常存在着对冗余向量依赖,导致测试应用时间增长,并产生额外的测试功耗等问题,提出一种新的低功耗确定测试向量发生器的综合算法.该向量发生器采用非一致细胞自动机的结构实现,利用基于模拟退火的动态邻域扩展算法寻找优化的细胞自动机的拓扑连接关系.对标准组合电路仿真实验的结果表明,所综合出的向量发生器可有效地产生给定的低功耗确定向量集,并且不影响原有的故障覆盖率和测试时间.     

Delphi环境下目标规划图解法算法程序的开发

作为目标规划算法平台的一个重要组成部分,研究了多目标线性规划中的目标规划图解法算法程序的开发,以方便该方法在各领域的应用。首先,介绍了目标规划的原理、建立目标规划模型的步骤。然后,介绍目标规划图解法的计算步骤以及在Del-phi环境下开发目标规划图解法的流程和具体方法。最后,通过实例对目标规划图解法算法程序的正确性进行了验证。所开发的算法程序直观简捷,方便工程人员的使用。     

基于移动Agent的电子商务交易平台研究

针对传统电子商务平台存在的问题,提出了一个以移动Agent为中介服务的电子商务交易平台,阐述了该平台各组成部分的功能.实验采用价格优先和数量优先的购买策略,分析了该平台中移动Agent的性能,实验仿真结果表明移动Agent的引入提高了平台处理客户信息的速度,缩短了达成交易的时间并降低了网络的通信量.