Development of gated fiber detectors for laser-induced strong electromagnetic pulse environments

With the development of laser technologies,nuclear reactions can happen in high-temperature plasma environments induced by lasers and have attracted a lot of at...     

《精细化工工艺》课程教学改革的研究

文章论述了合理组织教材以突出教材的重点,凸显知识体系的规律性;精讲教材内容,使学生抓住精细化工的实质;课堂教学与实验内容相结合以增强学生对精细化工理论和实验的理解;把科研内容引入教学中以激起学生的学习兴趣及对科学知识探求的热情;充分利用外部教学条件,把教学与精细化工生产实际结合起来,以培养学生的实际技能等方面的内容。     

Genome-Wide Identification of Switchgrass Laccases Involved in Lignin Biosynthesis and Heavy-Metal Responses

Plant laccase genes belong to a multigene family, play key roles in lignin polymerization, and participate in the resistance of plants to biotic and abiotic stresses. Switchgrass is an important resource for forage and bioenergy production, yet information about the switchgrass laccase gene family is scarce. Using bioinformatic approaches, a genome-wide analysis of the laccase multigene family in switchgrass was carried out in this study. In total, 49 laccase genes (PvLac1 to PvLac49) were identified; these can be divided into five subclades, and 20 of them were identified as targets of miR397. The tandem and segmental duplication of laccase genes on Chr05 and Chr08 contributed to the expansion of the laccase family. The laccase proteins shared conserved signature sequences but displayed relatively low sequence similarity, indicating the potential functional diversity of switchgrass laccases. Switchgrass laccases exhibited distinct tissue/organ expression patterns, revealing that some laccases might be involved in the lignification process during stem development. All five of the laccase isoforms selected from different subclades responded to heavy metal. The immediate response of lignin-related laccases, as well as the delayed response of low-abundance laccases, to heavy-metal treatment shed light on the multiple roles of laccase isoforms in response to heavy-metal stress.     

Systematic adsorption process of petroleum hydrocarbon by immobilised petroleum-degradation bacteria system in degradation pathways

In order to effectively control petroleum hydrocarbon pollution by immobilisation technology,it is necessary to understanding the degradation pathways of petrol...     

Role of ERAB/L-3-hydroxyacyl-coenzyme A dehydrogenase type II activity in Abeta-induced cytotoxicity

Endoplasmic reticulum-associated amyloid beta-peptide (Abeta)-binding protein (ERAB)/L-3-hydroxyacyl-CoA dehydrogenase type II (HADH II) is expressed at high levels in Alzheimer's disease (AD)-affected brain, binds Abeta, and contributes to Abeta-induced cytotoxicity. Purified recombinant ERAB/HADH II catalyzed the NADH-dependent reduction of S-acetoacetyl-CoA with a Km of approximately 68 microM and a Vmax of approximately 430 micromol/min/mg. The contribution of ERAB/HADH II enzymatic activity to Abeta-mediated cellular dysfunction was studied by site-directed mutagenesis in the catalytic domain (Y168G/K172G). Although COS cells cotransfected to overexpress wild-type ERAB/HADH II and variant beta-amyloid precursor protein (betaAPP(V717G)) showed DNA fragmentation, cotransfection with Y168G/K172G-altered ERAB and betaAPP(V717G) was without effect. We thus asked whether the enzyme might recognize alcohol substrates of which the aldehyde products could be cytotoxic; ERAB/HADH II catalyzed oxidation of a variety of simple alcohols (C2-C10) to their respective aldehydes in the presence of NAD+ and NAD-dependent oxidation of 17beta-estradiol. Addition of micromolar levels of synthetic Abeta(1-40) to purified ERAB/HADH II inhibited, in parallel, reduction of S-acetoacetyl-CoA (Ki approximately 1.6 microM), as well as oxidation of 17beta-estradiol (Ki approximately 3.2 microM) and (-)-2-octanol (Ki approximately 2.6 microM). Because micromolar levels of Abeta were required to inhibit ERAB/HADH II activity, whereas Abeta binding to ERAB/HADH II occurred at much lower concentrations (Km approximately 40-70 nM), the latter more closely simulating Abeta levels within cells, Abeta perturbation of ERAB/HADH II was likely to result from mechanisms other than the direct modulation of enzymatic activity. Cells cotransfected to overexpress ERAB/HADH II and betaAPP(V717G) generated malondialdehyde-protein and 4-hydroxynonenal-protein epitopes, which were detectable only at the lowest levels in cells overexpressing either ERAB/HADH II or betaAPP(V717G) alone. Generation of such toxic aldehydes was not observed in cells contransfected to overexpress Y168G/K172G-altered ERAB and betaAPP(V717G). We conclude that the generalized alcohol dehydrogenase activity of ERAB/HADH II is central to the cytotoxicity observed in an Abeta-rich environment.     

矿热炉把持器的传热分析

以矿热炉电极把持器的传热计算为核心,研究把持器的结构设计和参数选用。通过对冷却水流量、壁面厚度、水垢厚度、烟气温度等影响因素的研究,结合把持器传热边界条件,分析了在不同参数条件下传热变化趋势,为电极把持器的设计和应用提供了参考依据。     

高铁混凝土简支预应力箱梁施工技术研究

结合我国某高铁预应力混凝土简支箱梁采用后张法施工的过程,简要论述三项关键工序、高性能混凝土耐久性指标的保证措施、减小箱梁后期徐变的相关施工措施及注意要点,以确保满足高速铁路运营的安全性、高速度、高平顺性和舒适度.     

自蔓延高温合成材料中耗散结构及研究意义

在简介自蔓延高温合成技术和耗散结构论的基础上,分析和阐述了自蔓延高温合成材料中的耗散结构,探讨了自蔓延高温合成材料中耗散结构的研究意义。     

电荷束团非均匀密度分布引起的发射度增长

直线加速器中电荷束团的非线性效应是导致束流发射度增长的一个重要原因。文中给出了直线加速器中几种常见的非均匀密度分布的有限长空间电荷束团所具有的非线性自场能，即纵向非均匀电荷密度分布分别为水袋型，抛物线型，而横向密度分布为均匀型，水袋型，抛物线型及高期型等各种组合非均匀密度分布的自场能公式，并得到了由束团非线性的均匀电荷密度分布引起的束流发射度增长。