Binding properties of bovine colostral anti-dinitrophenyl (dnp) immunoglobulins g2 (igg2)

The number of combining sites per mole of bovine colostral anti-DNP IgG2 was found to be 2 and the total affinity constant was 0.81 X 10(4) M-1. Unlike bovine colostral IgG1, the nonspecific binding of H3-epsilon-DNP-1-lysine by IgG2 as a function of increased concentrations, did not show a negative, cooperative slope. Spectral measurements made with anti-DNP IgG2 in the reference cell vs. anti-DNP IgG2 plus hapten in the experimental cell revealed a hypochromic, red shift from 363 to 365 nm. If the reference cell contained hapten, a red shift from 280 to 283 nm and an enhancement in the extinction coefficient of IgG2 was observed. These spectral changes were not observed if nonspecific IgG2 was substituted for anti-DNP IgG2 in the experimental cell. The enhancement in the extinction coefficient was interpreted to be due to a possible exposure of previously buried tryosine and tryptophan residues.     

Studies on the antitesticular action of DL-6-(N-2-pipecolinomethyl)-5-hydroxy-indane (PMHI) in the rat

Both prepubertal and adult rats were treated with a single oral dose of either 60 mg or 120 mg of dl-6-(N-pipecolinomethyl)-5-hydroxy indane maleate (PMHI) per kg of body weight. Their testicular weights were drastically reduced compared with those of the controls. A follow-up, beginning on the third day post-treatment and continuing for a period of 50 days, showed that the body weight growth of PMHI-treated rats was not retarded. The hormonal profile indicated that, except for FSH which showed a transitory elevation in PMHI-treated immature rats, the serum levels of LH, estrogen, and testosterone were indistinguishable from those of the controls. Testicular histology revealed that the spermatogenic process in PMHI-treated rats recovered at a dose-related rate. EM sections of testes of adult rats indicated that cytoplasmic vacuolation appeared in the Sertoli cells 5 h post-treatment. The consequent cascade of arrested spermiogenesis included abnormal acrosomal condensation of spermatids and sloughing of polynucleated spermatids. Some spermatocytes also seemed to be affected, but spermatogonia and Leydig cells remained intact. These results indicate the PMHI acts primarily on Sertoli cells and causes arrest in the spermiogenetic stage of the spermatids. At a higher and toxic dose of PMHI, however, the earlier germinal elements might also be affected, due to the extensive damage to the supporting Sertoli cells.     

有界随机噪声参数激励下碰撞系统的矩稳定性

研究了单自由度线性单边碰撞系统在有界随机噪声参数激励下系统的矩稳定性问题.用Zhuravlev变换将碰撞系统转化为连续的非碰撞系统,然后用随机平均法得到了关于慢变量的随机微分方程.利用伊藤法则给出了系统一、二阶矩满足的常微分方程,根据微分方程的稳定性理论得到了系统一阶矩稳定充分必要条件的解析表达式和二阶矩稳定充分必要条件的数值算法,并对理论结果用数值方法进行了仿真计算.理论分析和数值仿真表明,无论是相对于一阶矩还是二阶矩的稳定性,随着随机激励振幅变大,系统的稳定性区域变小从而使得系统变得不稳定.而当调谐参数趋于零系统达到参数主共振情形时,系统的稳定性区域变得最小.当随机噪声强度逐渐变小趋于零时,由二种矩稳定性给出的稳定性区域变得一致.在一定的参数区域内,随机噪声使得系统稳定化.     

火试金重量法测定导电银浆中银量

报导运用火试金重量法测定导电银浆中银含量的方法。考察了称样载体、焙烧温度、铅箔用量、灰吹温度对银含量分析结果的影响,结果表明样品用铅箔作称重载体,250 ℃焙烧,15 g铅箔包裹,900 ℃灰吹是比较理想的方案。实验称取与样品中银量相当的金属银按试样的测定步骤和环境条件分析,银量的回收率作为补正因子k对试样分析结果进行补正。方法回收率为99.6 %～100.2%。对实际样品进行分析,测定值与推荐值相一致,相对标准偏差为0.51%和0.71 %（n=5）。     

新型食品试验箱的研制及其控温性能试验分析

新型食品试验箱是常压下工作的高低温型试验用小型制冷装置。为使箱内空气温度在给定的时间内达到-30℃,试验箱选用了高效顶置式冷风机和新冷媒全封闭低温压缩机和电子膨胀阀。试验箱同时配有全自动的微机数据采集系统能完成各项温控试验的数据记录。     

设计创造价值——首届中国家具原创设计大赛综述

<正>2014年4月18-20日,首届中国家具原创设计大赛作品展与第三届中国·苏州文化创意设计产业交易博览会(简称创博会)同期举行,它也是第三届中国·苏州创博会的重要活动之一。本次大赛由中国家具销售商联合会、江苏省家具协会、江苏蠡口家具城管委会、苏州文化创意产业联合会、苏州文化创意产业     

Effect of relative humidity on the store stability of spray-dried beta-carotene nanoemulsions

In order to overcome the limitations of liquid-base emulsion system, beta-carotene nanoemulsions stabilized by modified starch were spray-dried to powders after the emulsification process. The powders showed a good dissolution in water and the reconstituted emulsions had similar particle sizes with the fresh nanoemulsions. A 30 days storage test was carried out to investigate the effect of relative humility (RH) on the storage stability of beta-carotene powders at 25.0 °C. The beta-carotene degradation profiles over time were found to fit well with a Weibull model and also closely related to the film property of the matrix, moisture sorption property and glass transition temperature of the powder. The results showed that modified starches with lower film oxygen permeability had a higher retention of beta-carotene during storage. The glass transition temperature of powder in different RH also affected the rate of beta-carotene degradation. Overall these results provide useful information for choosing wall materials and storage conditions to protect nutraceuticals in delivery systems.     

2015科隆国际家具展和科隆国际厨房展回眸

<正>来自138个国家的146000名观众·国外观众明显增多·科隆展是全球最大的家具商业展之一2015科隆国际展和国际厨房展已在2015年1月25日的周六顺利闭幕。来自138个国家的146000名观众来到科隆展,在这其中有102000名来自德国国内外的贸易厂商(2013年是99000名厂商),这也意味着本届展会远超2014届展会。"这些数据说明了科隆这两个展会的国     

Selective quantification of trace palladium in road dusts and roadside soils by displacement solid-phase extraction online coupled with electrothermal atomic absorption spectrometry

There is a growing concern about the effect of palladium on human health because of the toxicity and increasing occurrence of palladium as a result of its extensive use in automotive catalytic converters. Development of reliable analytical methodologies for the determination of palladium in environmental materials is of great importance for critical evaluation of the possible risks for human health. In this work, a displacement solid-phase extraction technique was developed and online coupled to electrothermal atomic absorption spectrometry (ETAAS) for selective and sensitive determination of trace palladium in environmental samples without need of any special selective complexing agents, selective sorbents, and masking agents. The developed methodology involved the online formation of copper pyrrolidine dithiocarbamate (Cu-PDC), and the resultant Cu-PDC was extracted onto a microcolumn packed with the sorbent from a cigarette filter. Trace Pd(II) was selectively preconcentrated through loading the sample solution onto the microcolumn by online displacement reaction between Pd(II) and the extracted Cu-PDC on the microcolumn. The retained analyte was subsequently eluted with 40 microL of ethanol for online ETAAS determination. Interferences from coexisting heavy metal ions with lower stability of their PDC complexes relative to Cu-PDC were minimized. The tolerable concentrations of Cd-(II), Fe(III), Co(II), Mn(II), Cr(III), and Zn(II) were up to 2, 6, 40, 2, 1.5, and at least 100 mg L(-1), respectively. Compared with conventional solid-phase extraction methodology, the developed displacement solid-phase extraction protocol gave 2-4 orders of magnitude improvement in the maximum tolerable concentrations of coexisting heavy metal ions. With the consumption of only 2.8 mL of sample solution, an enhancement factor of 52 and a detection limit (3sigma) of 18 ng L(-1) were achieved at a sample throughput of 30 samples h(-1). The precision (RSD, n = 13) was 2.5% at the 1 microg L(-1) level. The present methodology was successfully applied to selective determination of trace palladium in local road dusts and roadside soils.     

超声波法提取板栗壳多糖的工艺条件优化

以板栗壳为原料,通过单因素实验,考察超声波功率、料液比、超声波处理时间对板栗壳多糖提取效果的影响。在单因素实验的基础上选取实验因素和水平,根据中心组合(CCD)实验设计原理,采用三因素三水平的响应面分析法,确定提取板栗壳多糖的最佳工艺条件为超声波功率为165W、料液比为1∶62、超声波处理时间为27min,在该条件下,超声波提取板栗壳多糖的效率最高,得率为11.48%。