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11.
采用边界压力单向条件的流函数旋度方程,对在不可压缩流体中贴近墙面平行移动的圆柱体周围的流场进行了二维数值仿真．计算结果与实验结果有较高的一致性,证明了在边界压力单向条件下流函数旋度方程的有效性． 相似文献
12.
MASAO TOKUNAGA MATSUJIRO ISHIBASHI DAISUKE TATSUDA HIROKO TOKUNAGA 《Yeast (Chichester, England)》1997,13(8):699-706
We constructed two mouse α-amylase secretion vectors for Kluyveromyces lactis using the well-characterized signal sequence of the pGKL 128 kDa killer precursor protein. Both PHO5 and PGK expression cassettes from Saccharomyces cerevisiae directed the expression of mouse α-amylase in YPD medium at a similar level of efficiency. K. lactis transformants secreted glycosylated and non-glycosylated α-amylase into the culture medium and both species were enzymatically active. The K. lactis/S. cerevisiae shuttle secretion vector pMI6 was constructed, and K. lactis MD2/1(pMI6) secreted about four-fold more α-amylase than S. cerevisiae YNN27 harboring the same plasmid, indicating that K. lactis is an efficient host cell for the secretion and production of recombinant proteins. © 1997 John Wiley & Sons, Ltd. 相似文献
13.
采用边界压力单向条件的流函数-旋度方程,对在不可压缩流体中贴近墙面平行移动的圆柱体周围的流场进行了二维数值仿真.计算结果与实验结果有较高的一致性,证明了在边界压力单向条件下流函数-旋度方程的有效性. 相似文献