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21.
22.
Jie-Long He An-Te Chen Jyong-Huei Lee Shih-Kang Fan 《International journal of molecular sciences》2015,16(9):22319-22332
The basic structural and functional unit of a living organism is a single cell. To understand the variability and to improve the biomedical requirement of a single cell, its analysis has become a key technique in biological and biomedical research. With a physical boundary of microchannels and microstructures, single cells are efficiently captured and analyzed, whereas electric forces sort and position single cells. Various microfluidic techniques have been exploited to manipulate single cells through hydrodynamic and electric forces. Digital microfluidics (DMF), the manipulation of individual droplets holding minute reagents and cells of interest by electric forces, has received more attention recently. Because of ease of fabrication, compactness and prospective automation, DMF has become a powerful approach for biological application. We review recent developments of various microfluidic chips for analysis of a single cell and for efficient genetic screening. In addition, perspectives to develop analysis of single cells based on DMF and emerging functionality with high throughput are discussed. 相似文献
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24.
Chenxu Liu Shuguang Zhang Ruonan Ji Peng Wang Jin Zhang Yu Tian Yonggang Meng Yedong He 《Ceramics International》2019,45(4):4747-4755
Semiconductor particles doped Al2O3 coatings were prepared by cathode plasma electrolytic deposition in Al(NO3)3 electrolyte dispersed with SiC micro- and nano-particles (average particle sizes of 0.5–1.7?µm and 40?nm respectively). The effects of the concentrations and particle sizes of the SiC on the microstructures and tribological performances of the composite coatings were studied. In comparison with the case of dispersing with SiC microparticles, the dispersion of SiC nanoparticles in the coatings was more uniform. When the concentration of SiC nanoparticles was 5?g/L, the surface roughness of the composite coating was reduced by 63%, compared with that of the unmodified coating. Friction results demonstrated that the addition of 5?g/L SiC nanoparticles reduced the friction coefficient from 0.60 to 0.38 and decreased the wear volume under dry friction. The current density and bath voltage were measured to analyze the effects of SiC particles on the deposition process. The results showed that the SiC particles could alter the electrical behavior of the coatings during the deposition process, weaken the bombardment of the plasma, and improve the structures of the coatings. 相似文献
25.
This calibration device of radio comprehensive tester is studied and established in this paper. It solves the verification and
calibration of a large number of general-purpose radio comprehensive testers. The calibration device consists of four calibration sub-
systems and calibration software system, including RF generator, audio generator, audio analyzer and RF analyzer. It also researches
on the configuration of standard equipment and analyzes the uncertainty of the calibration devices, ensuring accurate and reliable val-
ue transfer. 相似文献
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随着FPGA技术和电力电机技术的发展,FPGA+ARM的集成方式已经成为FPGA的发展方向.针对一体化多轴运动控制与驱动的特点,选用了集成双核ARM CPU与FPGA结合的Xilinx Zynq-7020全可编程System-on-chip(SoC)作为硬件平台,一个ARM CPU完成多轴的位置环、速度环和电流环的算法以及多轴轨迹生成,能同时完成伺服高级算法如谐振等,另一个ARM CPU完成交互功能,发挥FPGA高速运算的功能,完成6轴电流环流水线控制以及双采样双更新电流环算法.提高了系统整体带宽,实现多轴ns级同步精度以实现更精确的位置轨迹,驱控一体内部数据通过共享内存以及高速内部总线的方式进行交换,其传输速度更快、传输信息更加丰富. 相似文献
28.
在模具加工刀路编制时,很多用户会将C reo设计模型导入M aster C A M、C im atron等软件中进行加工刀路编制,往往引起很多数据转换的问题。文中采用C reo软件的一个加工模块N C A ssem bly进行无缝衔接,完成刀路编制。并结合一个实例进行了工艺分析,进而实现粗加工和精加工。 相似文献
29.
Kai He Zhe Li Longfei Liu Mengxi Zheng Prof. Chengde Mao 《Chembiochem : a European journal of chemical biology》2020,21(15):2132-2136
As a giant leap in DNA self-assembly, DNA origami has exhibited an unprecedented ability to construct nanostructures with arbitrary shapes and sizes. In typical DNA origami, hundreds of short DNA staple strands fold a long, single-stranded (ss) DNA scaffold cooperatively into designed nanostructures. However, large numbers of DNA strands are expensive and would hinder applications such as pharmaceutical investigations because of the complicated components. Therefore, one challenge is how to reduce the number of staple strands needed to construct DNA origami. For a DNA origami structure, the scale-free folding pattern of the scaffold strand is determined by staple strands at the branching vertexes. Simple duplex regions help to define the size-related features of the origami geometry. In this study, we hypothesized that a scaffold strand can be correctly folded into a designed topology by using only staple strands involved in branching vertexes. After assembly, any remaining, flexible, single-stranded regions of the scaffold could be converted into rigid duplexes by DNA polymerase to achieve the designed geometric structures. To demonstrate the concept, we used only 18 staple strands (covering 15 % of the scaffold strand) to assemble a porous DNA nanostructure, which was visualized by atomic force microscopy (AFM). This study helps understanding of the role of cooperativity in origami folding, and provides a cost-effective approach for small-scale prototyping DNA origami. 相似文献
30.
Baofeng Hou Hong He Ruixiu Jia Meilong Fu Yanbin Cao Shaoqun He Liwen Luo Youqing Huang 《应用聚合物科学杂志》2019,136(10):47161
The synthesis and performance of a novel temperature-tolerant foamed resin for enhanced oil recovery were investigated using various methods, including infrared, NMR, scanning electron microscopy (SEM), and displacement experiments. Polycondensation of furfuryl alcohol prepolymers was confirmed by the infrared and NMR results. The poor temperature tolerance of furfuryl alcohol prepolymers after gelation at high temperatures is mainly due to the fracture of furan rings. The addition of ester additives is an effective method of increasing the temperature tolerance of the prepared foamed resins and can effectively reduce the weight-loss rate of the polycondensation products. The SEM results show that the skeleton structure of the foamed resin remains intact after high-temperature treatment. Thus, the novel plugging agent system has excellent thermal stability and still has a high strength (>0.8 MPa) after high-temperature aging treatment for 40 days, giving the prepared foamed resin a good plugging performance (plugging rate > 91%) at 250 °C. © 2018 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019 , 136, 47161. 相似文献