全文获取类型
收费全文 | 102884篇 |
免费 | 1427篇 |
国内免费 | 1228篇 |
专业分类
电工技术 | 1926篇 |
综合类 | 138篇 |
化学工业 | 10502篇 |
金属工艺 | 5456篇 |
机械仪表 | 3193篇 |
建筑科学 | 2034篇 |
矿业工程 | 159篇 |
能源动力 | 2968篇 |
轻工业 | 6067篇 |
水利工程 | 705篇 |
石油天然气 | 668篇 |
武器工业 | 15篇 |
无线电 | 15947篇 |
一般工业技术 | 21763篇 |
冶金工业 | 25265篇 |
原子能技术 | 1385篇 |
自动化技术 | 7348篇 |
出版年
2022年 | 453篇 |
2021年 | 697篇 |
2020年 | 522篇 |
2019年 | 676篇 |
2018年 | 1132篇 |
2017年 | 1094篇 |
2016年 | 1173篇 |
2015年 | 924篇 |
2014年 | 1491篇 |
2013年 | 4675篇 |
2012年 | 2576篇 |
2011年 | 3844篇 |
2010年 | 3099篇 |
2009年 | 3720篇 |
2008年 | 3894篇 |
2007年 | 4083篇 |
2006年 | 3691篇 |
2005年 | 3327篇 |
2004年 | 3182篇 |
2003年 | 3026篇 |
2002年 | 2674篇 |
2001年 | 2970篇 |
2000年 | 2720篇 |
1999年 | 3076篇 |
1998年 | 9443篇 |
1997年 | 6159篇 |
1996年 | 4764篇 |
1995年 | 3163篇 |
1994年 | 2789篇 |
1993年 | 2723篇 |
1992年 | 1631篇 |
1991年 | 1594篇 |
1990年 | 1520篇 |
1989年 | 1324篇 |
1988年 | 1172篇 |
1987年 | 863篇 |
1986年 | 888篇 |
1985年 | 916篇 |
1984年 | 803篇 |
1983年 | 698篇 |
1982年 | 700篇 |
1981年 | 681篇 |
1980年 | 567篇 |
1979年 | 476篇 |
1978年 | 414篇 |
1977年 | 533篇 |
1976年 | 959篇 |
1975年 | 300篇 |
1974年 | 276篇 |
1973年 | 256篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
101.
A stable subunit of Maackia amurensis leukoagglutinin (MMAL) was prepared by the selective reduction of disulfide bridges between the subunits followed by alkylation with 4-vinylpyridine. MMAL failed to precipitate fetuin, whereas it retained its ability to bind to the same glycoprotein coated on a plastic plate, indicating the monovalency of this derivative. This binding to immobilized fetuin was inhibited by a haptenic sugar, Neu5Ac alpha 2-3lactose, with the same inhibitory potency as against the native M. amurensis leukoagglutinin. Microscopic observation as well as flow cytometric analyses showed that Chinese hamster ovary cells were clearly stained with fluorescein isothiocyanate-labeled MMAL without any detectable agglutination. This staining was inhibited by the addition of fetuin or by the sugar chains of fetuin. Differences in the types of sialylated glycoconjugates on the cell surface of several cell lines were detected by the combined use of fluorescein isothiocyanate-labeled MMAL and the monomeric derivative of elderberry bark lectin (specific for the Neu5Ac alpha 2-6Gal/GalNAc sequence) by flow cytometry. These results demonstrate the usefulness of these monovalent derivatives of sialylated oligosaccharide-specific lectins as probes for the analysis of cell surface glycoconjugates containing sialic acid by the technique of flow cytometry. 相似文献
102.
Effects of 5-fluorouracil (5-FU) and UFT on an experimental liver metastasis model were compared at equi-effective dosage levels against subcutaneous tumor of mouse colon 26. 5-FU at the dosage level of 40 mg/kg suppressed the subcutaneous tumor growth by 70.0% and 45.0% on day 13 and day 18, respectively, and UFT at 20 mg/kg provided almost equal suppression (63.0% and 48.0%). In the liver metastasis model, 5-FU at 40 mg/kg showed more potent prevention of the formation of metastatic foci (94.9%) than did UFT (60.4%) at 20 mg/kg. 5-FU at 40 mg/kg produced a much higher peak serum level of 5-FU than did UFT at 20 mg/kg and also showed a much higher AUC (area under the curve) level in the portal blood. These results suggest that oral administration of 5-FU might be useful in prevention of liver metastasis of colorectal cancer. 相似文献
103.
Y Furuya Y Yoshida T Katayama S Yamamoto A Kawamura 《Canadian Metallurgical Quarterly》1993,31(6):1637-1640
Polymerase chain reaction (PCR) with nested primer pairs was used to diagnose scrub typhus and identify the Rickettsia tsutsugamushi serotype. The primer pairs used for PCR were designed on the basis of the nucleotide sequence of the gene that encodes the 56-kDa antigen. Serotype-specific primers were used in the second PCR amplification. Five serovariants, the Gilliam, Karp, Kato, Kawasaki, and Kuroki strains of R. tsutsugamushi, were identified by nested PCR. In addition, the serotype identified by PCR with DNA from blood clots was the same as that of the strain isolated from five patients with scrub typhus. These findings indicate that this method is useful for diagnosis and identification of the rickettsial serotype in infected patients. 相似文献
104.
The preembedding double immunoreaction method was used to study interrelations of enkephalinergic and GABAergic neuronal elements in the dorsal raphe nucleus of the Wistar albino rat. The enkephalin-like neuronal elements were immunoreacted by the peroxidase-antiperoxidase method and silver-gold intensified, which showed strongly and was specific. The GABA-like immunoreactive neurons were immunoreacted by the peroxidase-antiperoxidase method only. GABA-like neural somata were postsynaptic to both the enkephalin-like immunoreactive and the non-immunoreactive axon terminals. The enkephalin-like immunoreactive axon terminals were also found to synapse GABA-like immunoreactive dendrites. The GABA-like immunoreactive neuronal elements were also found to receive synapses from other non-immunoreactive as well as GABA-like immunoreactive axon terminals. Almost all of the synapses appeared to be asymmetrical. Possible functional activity of interactions among the enkephalinergic, GABAergic, and serotonergic neuronal elements in the dorsal raphe nucleus are discussed. 相似文献
105.
Various wireless systems and the research preceding their practical use in China are described. The topics discussed include research establishments, research and development projects underway, challenges inherent to digital radio, and expansion opportunities afforded by satellite communications 相似文献
106.
Catastrophic convolutional codes (CC) cause an infinite number of decoded data bit errors when decoding a finite number of code symbols. A CC displays a catastrophic error propagation if the generating polynomials have a common factor. An efficient algorithm for polynomial factorization in GF(2m) is used for detecting catastrophic CC for any rate n/m and constraint length k. A general formula is derived to calculate the number of catastrophic codes in any (m, n, k) CC. 相似文献
107.
The magnetization of a melt-texture growth (MTG) HTSC ring has been studied. It is shown that the magnetic field inside the ring is larger than the external field under a certain range of external magnetic fields. We have also investigated the magnetic field dependence of the response of a detective coil near a rotating superconducting ring. The responses of the MTG sample are different for different cooling methods. 相似文献
108.
Tajiri A. Minakuchi K. Komeda K. Bessho Y. Inoue Y. Yodoshi K. Yamaguchi T. 《Electronics letters》1993,29(4):369-370
A highly reliable 200 mW AlGaAs laser diode with a fundamental transverse mode has been developed, by optimising its structure with a 0.8 mu m thick p-cladding layer, a 1200 mu m long cavity length, and a front facet coating with a low reflectivity of 2%. The maximum output power was 500 mW, and stable fundamental transverse mode operation was obtained up to 350 mW. Stable operation under 200 mW and 50 degrees C was confirmed for more than 1200 h. Optical feedback noise was below 3*10/sup -14/ Hz/sup -1/.<> 相似文献
109.
K. Nomoto H. Saitoh A. Chida H. Sannomiya M. Itoh Y. Yamamoto 《Solar Energy Materials & Solar Cells》1994,34(1-4)
a-Si alloy three-stacked solar cells have been studied to improve the stabilized efficiency of a-Si: H based solar cells. Based on the analysis by the individual characterization method of the component cells in stacked type cells, the a-Si :H middle cell was replaced with an a-SiGe :H cell. Furthermore, the optical confinement technology was improved to obtain a high-output current with thin i-layer thickness in the a-SiGe :H bottom cell. By this device design, the initial conversion efficiency was improved up to 12.4% and more than a 10% stabilized efficiency was obtained in a-SiC :H/a-SiGe :H/a-SiGe :H three-stacked cells. These cell characteristics were confirmed by measurements at the JQA Organization (the former JMI Institute). 相似文献
110.
C Llanes P Gabant M Couturier Y Michel-Briand 《Canadian Metallurgical Quarterly》1994,176(11):3403-3407
The Inc A/C plasmids, like Inc P and Inc Q plasmids, have a broad host range. However, their maintenance functions remain to be studied. An autoreplicative region of 2.79 kb named RepA/C, able to replicate both in the family Enterobacteriaceae and in Pseudomonas spp., was isolated and sequenced. The stability, copy number, and incompatibility expression of this replicon were determined. RepA/C and a nonautoreplicative fragment of 16 kb of this replicon were used as probes and showed specific hybridizations with the Inc P3-A/C plasmids from Pseudomonas spp. and members of the Enterobacteriaceae. These probes could be used as tools for identification of the plasmids of this epidemiologically important Inc group. 相似文献