Poly (hydroxyethyl methacrylate-glycidyl methacrylate) films modified with different functional groups: In vitro interactions with platelets and rat stem cells

Copolymerization of 2-hydroxyethylmethacrylate (HEMA) with glycidylmethacrylate (GMA) in the presence of α-α′-azoisobisbutyronitrile (AIBN) resulted in the formation of hydrogel films carrying reactive epoxy groups. Thirteen kinds of different molecules with pendant NH₂ group were used for modifications of the p(HEMA-GMA) films. The NH₂ group served as anchor binding site for immobilization of functional groups on the hydrogel film via direct epoxy ring opening reaction. The modified hydrogel films were characterized by FTIR, and contact angle studies. In addition, mechanical properties of the hydrogel films were studied, and modified hydrogel films showed improved mechanical properties compared with the non-modified film, but they are less elastic than the non-modified film. The biological activities of these films such as platelet adhesion, red blood cells hemolysis, and swelling behavior were studied. The effect of modified hydrogel films, including NH₂, (using different aliphatic CH₂ chain lengths) CH₃, SO₃H, aromatic groups with substituted OH and COOH groups, and amino acids were also investigated on the adhesion, morphology and survival of rat mesenchymal stem cells (MSCs). The MTT colorimetric assay reveals that the p(HEMA-GMA)-GA-AB, p(HEMA-GMA)-GA-Phe, p(HEMA-GMA)-GA-Trp, p(HEMA-GMA)-GA-Glu formulations have an excellent biocompatibility to promote the cell adhesion and growth. We anticipate that the fabricated p(HEMA-GMA) based hydrogel films with controllable surface chemistry and good stable swelling ratio may find extensive applications in future development of tissue engineering scaffold materials, and in various biotechnological areas.     

Lead levels in deciduous teeth of children from urban and suburban regions in Ankara (Turkey)

PURPOSE: To determine whether hyperglycemia affects pancreatic islet microcirculation in vivo and whether nitric oxide is a mediator. METHODS: Islet blood flow was measured before and after infusion of glucose during in vivo microscopy of mouse pancreatic islet. The pancreas of male BALB/c mice was exteriorized and viewed under the microscope utilizing monochromatic transmitted light. The carotid artery and tail vein were cannulated and systemic blood pressure was monitored continuously. Under fluorescent light, a 0.02 mL bolus of 2% fluorescein isothyocyanate (FITC-albumin) was injected intra-arterially and the first pulse of FITC-albumin through an islet capillary was videorecorded. Following equilibration, either glucose or normal saline 300 mg/g of body weight was given intravenously. Five minutes later, a second bolus was given and the second pulse was videorecorded. The study was repeated in the presence of N omega-nitro-L-arginine methyl ester (L-NAME). The FITC-albumin bolus mean transit time (TT) and observed cross time (OCT) through the islet were calculated using slow-motion video analysis of the recorded images. RESULTS: Infusion of glucose resulted in a significant increase in islet blood flow with no change in systemic blood pressure: baseline TT was 20 +/- 1.3 pixel/0.03 sec and baseline OCT was 0.6 +/- 0.04 seconds; during hyperglycemia, TT was 16.1 +/- 1 pixel/0.03 sec, and OCT was 0.48 +/- 0.03 seconds (n = 11, P < 0.05 versus basal via paired t-test). Continuous infusion of L-NAME negated the effect of hyperglycemia on islet blood flow: baseline TT was 20 +/- 1.8 pixel/0.03 sec and OCT was and 0.6 +/- 0.05 seconds; during hyperglycemia, TT was 20 +/- 1.1 pixel/0.03 sec and OCT was 0.6 +/- 0.33 seconds (n = 10; P < 0.05 versus glucose via unpaired t-test).     

A new facial expression recognition based on curvelet transform and online sequential extreme learning machine initialized with spherical clustering

In this paper, a novel algorithm is proposed for facial expression recognition by integrating curvelet transform and online sequential extreme learning machine (OSELM) with radial basis function (RBF) hidden node having optimal network architecture. In the proposed algorithm, the curvelet transform is firstly applied to each region of the face image divided into local regions instead of whole face image to reduce the curvelet coefficients too huge to classify. Feature set is then generated by calculating the entropy, the standard deviation and the mean of curvelet coefficients of each region. Finally, spherical clustering (SC) method is employed to the feature set to automatically determine the optimal hidden node number and RBF hidden node parameters of OSELM by aim of increasing classification accuracy and reducing the required time to select the hidden node number. So, the learning machine is called as OSELM-SC. It is constructed two groups of experiments: The aim of the first one is to evaluate the classification performance of OSELM-SC on the benchmark datasets, i.e., image segment, satellite image and DNA. The second one is to test the performance of the proposed facial expression recognition algorithm on the Japanese Female Facial Expression database and the Cohn-Kanade database. The obtained experimental results are compared against the state-of-the-art methods. The results demonstrate that the proposed algorithm can produce effective facial expression features and exhibit good recognition accuracy and robustness.     

In vitro cadmium removal from human serum by Cibacron Blue F3GA–thionein‐complex conjugated affinity membranes

A new membrane affinity biosorbent carrying thionein has been developed for selective removal of cadmium ions from human serum. Microporous poly(2‐hydroxyethyl methacrylate) (pHEMA) membranes were prepared by photopolymerization of HEMA. The pseudo dye ligand Cibacron Blue F3GA (CB) was covalently immobilized on the pHEMA membranes. Then, the cysteine‐rich metallopeptide thionein was conjugated onto the CB‐immobilized membrane. The maximum amounts of CB immobilized and thionein conjugated on the membranes were 1.07 µmol cm⁻² and 0.92 µmol cm⁻², respectively. The hydrophilic pHEMA membrane had a swelling ratio of 58% (w/w) with a contact angle of 45.8 °. CB‐immobilized and CB‐immobilized–thionein‐conjugated membranes were used in the Cd(II) removal studies. Cd(II) ion adsorption appeared to reach equilibrium within 30 min and to follow a typical Langmuir adsorption isotherm. The maximum capacity (q _m) of the CB‐immobilized membranes was 0.203 (µmol Cd(II)) cm⁻² membrane and increased to 1.48 (µmol Cd(II)) cm⁻² upon CB–thionein‐complex conjugation. The pHEMA membranes retained their cadmium adsorption capacity even after 10 cycles of repeated use. © 2000 Society of Chemical Industry     

Environmental flow assessment for energy generation sustainability employing different hydraulic evaluation methods: Çambaşi hydropower plant case study in Turkey

The operating principle of hydroelectric power plants (HPP) is based on utilizing the potential energy of water, which constitutes the basic component of the plant. In other words, water is crucially important in energy production in hydroelectric power plants. The importance of water is not only limited to the energy production, but also can affect directly or indirectly all living things in the water basin. This study deals with the methods of determining the environmental flow, which will not damage the integrity of the rivers and the ecosystem in HPPs, together with enabling the sustainable electricity production as an indirect result. Suggestions are made for determining the environmental flow for Çambas? regulator and Hydropower plant, a run-of-river type power plant in Turkey, after reviewing commonly used methods all over the world. Tennant, Tessmann, and flow duration curve methods were used in determining the environmental flow. Separate calculations were carried out for Cambas? and Ogene regulators, which constitute the hydropower plant. The calculated values were compared with the normal (regime) flow rates and project flow values. As a result, Tessmann method, one of the hydrological based environmental flow determination methods, and good category of Tennant method, 20 % of annual average flow in dry period and 40 % of annual average flow in wet period, are proposed for Çamba?? HPP.     

Dephytinization of food stuffs by phytase of Geobacillus sp. TF16 immobilized in chitosan and calcium-alginate

In this work, Geobacillus sp. TF16 phytase was separately immobilized in chitosan and Ca-alginate with the efficiency of 38% and 42%, respectively. These enzymes exhibited broad substrate specificity. Maximal relative phytase activity was measured at pH 5.0 and 95°C and pH 3.0 and 75°C for chitosan and Ca-alginate, respectively. The enzymes were highly stable in a wide pH and temperature range. Values of K_m and V_max were determined as 2.38 mM and 3401.36 U/mg protein for chitosan, and 7.5 mM and 5011.12 U/mg protein for Ca-alginate. The immobilized enzymes showed higher resistance to proteolysis. After 4 h incubation, hydrolysis capacities of chitosan- and Ca-alginate immobilized enzymes for soymilk phytate were calculated as 24% and 33%, respectively. The chitosan- and Ca-alginate immobilized phytases conserved its original activity after 8 and 6 cycles of reuse, respectively. The features of the enzymes were very attractive and they might be useful for some industrial applications.     

Performance characteristics of a low heat rejection diesel engine operating with biodiesel

Vegetable oils are a promising alternative among the different diesel fuel alternatives. However, the high viscosity, poor volatility and cold flow characteristics of vegetable oils can cause some problems such as injector coking, severe engine deposits, filter gumming, piston ring sticking and thickening of lubrication oil from long-term use in diesel engines. These problems can be eliminated or minimized by transesterification of the vegetable oils to form monoesters. These monoesters are known as biodiesel. The important advantages of biodiesel are lower exhaust gas emissions and its biodegradability and renewability compared with petroleum-based diesel fuel. Although the transesterification improves the fuel properties of vegetable oil, the viscosity and volatility of biodiesel are still worse than that of petroleum diesel fuel. The energy of the biodiesel can be released more efficiently with the concept of low heat rejection (LHR) engine. The aim of this study is to apply LHR engine for improving engine performance when biodiesel is used as an alternative fuel. For this purpose, a turbocharged direct injection (DI) diesel engine was converted to a LHR engine and the effects of biodiesel (produced from sunflower oil) usage in the LHR engine on its performance characteristics have been investigated experimentally. The results showed that specific fuel consumption and the brake thermal efficiency were improved and exhaust gas temperature before the turbine inlet was increased for both fuels in the LHR engine.     

Purification and characterization of a novel thermostable phytase from the thermophilic Geobacillus sp. TF16

A novel phytase from thermophilic Geobacillus sp. TF16 was puri?ed approximately 5-fold using ammonium sulfate precipitation and ion exchange chromatography, and determined as a single band 106.04 kDa on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Optimum temperature and optimum pH were found to be 85°C and 4.0, respectively. The enzyme is highly thermostable and V_max and K_m values were calculated as 526.28 U/mg and 1.31 mM, respectively. It was also found that the enzyme exhibited a broad substrate selectivity and resistance toward proteases and effectively hydrolyzed soymilk phytate. These results suggest that this study provides an alternative phytase enzyme with enhanced properties.     

CdO/Cu2O solar cells by chemical deposition

CdO and Cu₂O thin films have been grown on glass substrates by chemical deposition method. Optical transmittances of the CdO and Cu₂O thin films have been measured as 60–70% and 3–8%, respectively in 400–900 nm range at room temperature. Bandgaps of the CdO and Cu₂O thin films were calculated as 2.3 and 2.1 eV respectively from the optical transmission curves. The X-ray diffraction spectra showed that films are polycrystalline. Their resistivity, as measured by Van der Pauw method yielded 10⁻²–10⁻³ Ω cm for CdO and approximately 10³ Ω cm for Cu₂O. CdO/Cu₂O solar cells were made by using CdO and Cu₂O thin films. Open circuit voltages and short circuit currents of these solar cells were measured by silver paste contacts and were found to be between 1–8 mV and 1–4 μA.