Biomedical applications of mRNA nanomedicine

As an attractive alternative to plasmid DNA, messenger RNA (mRNA) has recently emerged as a promising class of nucleic acid therapeutics for biomedical applications. Advances in addressing the inherent shortcomings of mRNA and in the development of nanoparticle-based delivery systems have prompted the development and clinical translation of mRNA-based medicines. In this review, we discuss the chemical modification strategies of mRNA to improve its stability, minimize immune responses, and enhance translational efficacy. We also highlight recent progress in nanoparticle-based mRNA delivery. Considerable attention is given to the increasingly widespread applications of mRNA nanomedicine in the biomedical fields of vaccination, protein-replacement therapy, gene editing, and cellular reprogramming and engineering.     

Large capacitance and fast polarization response of thin electrolyte dielectrics by spin coating for two-dimensional MoS2 devices

Nano Research - A spin-coating method was applied to obtain thinner and smoother PEO/LiClO4 polymer electrolyte films (EFs) with a lower level of crystallization than those obtained using a...     

Entrapping multifunctional dendritic nanoparticles into a hydrogel for local therapeutic delivery and synergetic immunochemotherapy

Nano Research - Developing multifunctional nanoparticles to support new therapy models is a promising and challenging task to address the current dilemma on antitumor treatment. Herein, we...     

Aggregation-induced emission luminogen-assisted stimulated emission depletion nanoscopy for super-resolution mitochondrial visualization in live cells

Nano Research - Aggregation-induced emission luminogens (AIEgens) are fluorescent agents that are ideal for bioimaging and have been widely used for organelle targeting, cellular mapping, and...     

Escherichia coli prlC gene encodes a trypsin-like proteinase regulating the cell cycle

BACKGROUND AND PURPOSE: Mood disorders are common after stroke and may impede physical, functional, and cognitive recovery, making early identification and treatment of potential importance. We aimed to compare the accuracy of the General Health Questionnaire (GHQ-30) and the Hospital Anxiety and Depression (HAD) Scale in detecting psychiatric morbidity after stroke and to determine the most suitable cutoff points for different purposes. METHODS: One hundred five hospital-referred stroke patients completed both the GHQ-30 and HAD Scale 6 months after onset before a blinded psychiatric assessment in which the Schedule for Affective Disorders and Schizophrenia with some supplementary questions was used to determine a DSM-IV (Diagnostic and Statistical Manual of Mental Disorders, Fourth Edition) diagnosis. Measures were compared in terms of sensitivity, specificity, and receiver operating characteristic curves. RESULTS: No significant differences were found between the GHQ-30 and the HAD Scale in identifying those patients with any DSM-IV diagnosis (P=0.95), grouped depression (P=0.56), or anxiety (P=0.25) disorders. The previously recommended cutoff points for identifying "cases" for the GHQ (4/5) and for the HAD Scale (8/9 and 11/12) were found to be suboptimal in this population. CONCLUSIONS: The GHQ-30 and HAD scale exhibited similar levels of sensitivity and specificity. Data are presented, taking into account the "cost" of false-positives and negatives, to allow a choice of cutoff points suitable for differing situations.     

Mechanical behaviour of metal–matrix composite deposits

The electroplating technique is used for producing thin sheets of copper- or nickel-based composites containing different volume fractions of -alumina dispersions. The microhardness and tensile behaviour of such composites, in both the as-deposited and the annealed state, are characterized. The strengthening mechanism of electroplated composites is found to be a combination of Orowan-type strengthening and the Hall–Petch effect.     

基于多目视觉的变形钢板三维测量方法

提出了一种使用4个相机测量变形钢板三维形状的方法;在没有纹理的钢板上画一些点,使用4个相机同时拍摄钢板;利用边缘检测算法找出钢板的边缘,利用特征点提取算法提取出4个图像中钢板上的特征点,并找到4个图像上精确的特征点匹配关系,然后测量出这些特征点的三维点;最后,以这些三维点为种子点向外区域增长,从而得到钢板的三维形状;实验对变形钢板进行三维测量,得到了钢板的精确的三维形状,结果验证了该方法的有效性和可行性。     

Matlab符号代数方程求解函数的改进及其应用

Matlab求解符号代数方程的函数solve可能产生方程解输出混乱的问题。采用字符串演算函数eval及动态结构数组加以改进,建立了求解符号代数方程的函数SolEqu。理论分析和算例表明,函数SolEqu不仅避免了直接使用solve可能导致的错误结果,而且调用更加方便,其使用范围也与函数solve完全相同,可广泛用于一般代数方程的求解。     

取代酚对日本长腿蛙蝌蚪急性毒性的QSAR研究

用Hartree-Fock(HF)和DFT-B3LYP方法,分别在较高基组6-31G**和6.311G**水平下,全优化计算了21种取代酚化合物.从中获得分予最高占用和最低空轨道能(EHOMO和ELUMO)、前线轨道能级差(△E＝EHOMO-ELUMO)、分子总能量(ET)、氧原了所带的最高正电荷(QH+)、最负原子的静电荷(Q-)、分子偶极矩(μ)和分子体积(V)等描述符.结合文献中标题化合物对日本长腿蛙蝌蚪的毒性值(-logLC50)和辛醇/水分配系数(logKow),由线性同归方法成功建立包含10gKow、ELUMO和Q-的三参数QSAR模型.其中,模型(5)的复相关系数R2=0.944 8,交叉验证系数Q2=0.920 5,标准偏差SE=0.187,Fisher检验值F=98.390,故其预测能力较好.由此推断,标题物对日本长腿蛙蝌蚪的毒性作用分为2步,首先穿过细胞壁在细胞内富集,以logKow和Q-描述;其次与亲核试剂发生亲电反应,以风ELUMO表示.     

The establishment of edge-based loop algebra theory of kinematic chains and its applications

Based on the edge-based array representation of loops in the topological graphs of kinematic chains, this paper first proposes three arithmetic operations of loops. Then the concept of the independent loop set as well as its determination rules is introduced, and a new structure decomposition algorithm of kinematic chains is presented. Based on the algorithm, an automatic and efficient method for rigid sub-chain detection and driving pair selection of kinematic chains is proposed. Finally, an index is proposed to assess computation complexity of kinematic analysis with respect to different driving pair selections.