酒石酸还原Cu(Ⅱ)制备多种形貌Cu_2O晶体及形成机理

以CuSO_4·5H_2O为原料,KOH为添加剂,酒石酸为还原剂,在水热条件下通过调整相关的实验参数,制备了一系列不同形态的Cu_2O微-纳米晶体及三维十字形枝晶。采用XRD、SEM等手段对不同形态的Cu_2O晶体进行了表征,探讨了不同因素变化对Cu_2O晶体形态的影响。分析认为,晶体生长过程控制了晶体的具体形貌和大小,Cu_2O晶体显露的单形晶面随晶体生长条件的变化而不同。在低温、弱碱性、高浓度溶液中有利于形成八面体形态的Cu_2O晶体,而菱形十二面体单形晶面在高温、强碱性、低浓度溶液中形成的晶体中显露的面积有所增大。     

Activating a zymogen without proteolytic processing: mutation of Lys15 and Asn194 activates trypsinogen

The zymogen and mature enzyme forms of trypsin-like serine proteases exhibit a wide range of activities. The prototypical trypsinogen-trypsin system is an example of a minimally active zymogen and a maximally active mature protease. The present work identifies several features of trypsinogen which govern its activity. Our results indicate that rat trypsin is 10(8)-fold more active than rat trypsinogen. Rat trypsinogen appears to be less active than bovine trypsinogen. His40 is believed to be an important determinant of zymogen activity. We are unable to verify this role for His40 in trypsinogen since the mutation of His40 to Phe appears to change the trypsin-substrate interface. Deletion of the N-terminal Ile16 from trypsin is expected to produce a trypsinogen-like protein since the Ile16-Asp194 salt bridge cannot form. Such mutants have higher activity and BPTI affinity than trypsinogen, which indicates that the activation peptide stabilizes the inactive trypsinogen conformation. The mutation of Lys15 to Ala increases the BPTI affinity and activity of trypsinogen to an even greater extent; thus, removal of Lys15 can account for the effect of the loss of the activation peptide. These results suggest that Lys15 is an important determinant of zymogen activity. The mutation of Asp194 to Asn also increases the BPTI affinity and activity of trypsinogen. This result suggests that in addition to stabilizing the active conformation of trypsin via the Ile16-Asp194 salt bridge, Asp194 also maintains the inactive conformation of trypsinogen. A correlation exists between the values of kcat/Km and BPTI affinity of mutant trypsinogens and trypsins. However, the slope of this correlation is 0.64, which indicates that different "active" conformations are involved in BPTI binding and substrate hydrolysis. DeltaI16V17 trypsinogen is the lone outlier; its BPTI affinity is higher than would be expected based on the value of kcat/Km. We show that the rate of BPTI association is slower for DeltaI16V17 trypsinogen than for a mutant trypsinogen with a similar BPTI affinity. This observation suggests that BPTI binds to an "active" trypsinogen conformation that is not kinetically accessible to substrates.     

Escherichia coli prlC gene encodes a trypsin-like proteinase regulating the cell cycle

BACKGROUND AND PURPOSE: Mood disorders are common after stroke and may impede physical, functional, and cognitive recovery, making early identification and treatment of potential importance. We aimed to compare the accuracy of the General Health Questionnaire (GHQ-30) and the Hospital Anxiety and Depression (HAD) Scale in detecting psychiatric morbidity after stroke and to determine the most suitable cutoff points for different purposes. METHODS: One hundred five hospital-referred stroke patients completed both the GHQ-30 and HAD Scale 6 months after onset before a blinded psychiatric assessment in which the Schedule for Affective Disorders and Schizophrenia with some supplementary questions was used to determine a DSM-IV (Diagnostic and Statistical Manual of Mental Disorders, Fourth Edition) diagnosis. Measures were compared in terms of sensitivity, specificity, and receiver operating characteristic curves. RESULTS: No significant differences were found between the GHQ-30 and the HAD Scale in identifying those patients with any DSM-IV diagnosis (P=0.95), grouped depression (P=0.56), or anxiety (P=0.25) disorders. The previously recommended cutoff points for identifying "cases" for the GHQ (4/5) and for the HAD Scale (8/9 and 11/12) were found to be suboptimal in this population. CONCLUSIONS: The GHQ-30 and HAD scale exhibited similar levels of sensitivity and specificity. Data are presented, taking into account the "cost" of false-positives and negatives, to allow a choice of cutoff points suitable for differing situations.     

An advanced computer code for single-tuned harmonic filter design

The objective of this paper is to present a novel approach for limiting harmonic and improving reactive compensation for industrial power systems using single-tuned filters. The unique feature of the developed power distribution model is that it incorporates both the filter capacity and resonant point as design parameters. The model also considers the detuning effects due to temperature and frequency variations, as well as manufacturing tolerances. The proposed approach was implemented as a PC-based computer code, and it was integrated with a user-friendly graphical user interface. The developed analysis tool was employed to solve the harmonic problems of several industrial plants, and the results all showed great improvements after using this computer tool     

Calculations of fracture criteria by classical mechanics I — Pellini's Duplex Rule

In a previous paper we presented a relaxation method to compute the elastic strain concentration of a crack. It was also shown, based on this method and in conjunction with the Rule of Sliding, that the plastic strain concentration curve can be calculated. In the current paper, we present the calculations of static load fracture using the relaxation method that we have previously proposed and the fracture criterion, f, of the second strength theory. Our results are in good agreement with those predicted by Pellini's Duplex Rule. We thus provide a theoretical basis for the Fracture Arrest Diagram of Pellini.