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排序方式: 共有1014条查询结果,搜索用时 15 毫秒
81.
Tomoharu Suzuki Shigeru Oshio Mari Iwata Hisayo Saburi Takashi Odagiri Tadashi Udagawa Isamu Sugawara Masakazu Umezawa Ken Takeda 《Particle and fibre toxicology》2010,7(1):1-8
Background
Epidemiological studies suggest that inhalation of carbonaceous particulate matter from biomass combustion increases susceptibility to bacterial pneumonia. In vitro studies report that phagocytosis of carbon black by alveolar macrophages (AM) impairs killing of Streptococcus pneumoniae. We have previously reported high levels of black carbon in AM from biomass smoke-exposed children and adults. We therefore aimed to use a mouse model to test the hypothesis that high levels of carbon loading of AM in vivo increases susceptibility to pneumococcal pneumonia.Methods
Female outbred mice were treated with either intranasal phosphate buffered saline (PBS) or ultrafine carbon black (UF-CB in PBS; 500 μg on day 1 and day 4), and then infected with S. pneumoniae strain D39 on day 5. Survival was assessed over 72 h. The effect of UF-CB on AM carbon loading, airway inflammation, and a urinary marker of pulmonary oxidative stress was assessed in uninfected animals.Results
Instillation of UF-CB in mice resulted a pattern of AM carbon loading similar to that of biomass-smoke exposed humans. In uninfected animals, UF-CB treated animals had increased urinary 8-oxodG (P = 0.055), and an increased airway neutrophil differential count (P < 0.01). All PBS-treated mice died within 72 h after infection with S. pneumoniae, whereas morbidity and mortality after infection was reduced in UF-CB treated animals (median survival 48 h vs. 30 h, P < 0.001). At 24 hr post-infection, UF-CB treated mice had lower lung and the blood S. pneumoniae colony forming unit counts, and lower airway levels of keratinocyte-derived chemokine/growth-related oncogene (KC/GRO), and interferon gamma.Conclusion
Acute high level loading of AM with ultrafine carbon black particles per se does not increase the susceptibility of mice to pneumococcal infection in vivo. 相似文献82.
In this paper, a back propagation neural network (BPNN) has been applied to predict the corner wear of a high speed steel (HSS) drill bit for drilling on different workpiece materials. Specially defined static and dynamic features extracted by a wavelet packet transform (WPT) from the resultant force converted from thrust and torque together with the cutting conditions (workpiece material, spindle speed, drill diameter, feed rate) are used as inputs to train the network to obtain a better output, drill corner wear. Drilling experiments have been carried out over a wide range and, features newly defined and conventional ones, features extracted from different frequency bands are compared. 相似文献
83.
Yasuo Watanabe Masahiro Yamaguchi Jun Sakamoto Youichi Tamai 《Yeast (Chichester, England)》1993,9(3):213-220
Plasma membrane was isolated from the salt-tolerant yeast Candida versatilis and the ATPase in plasma membrane was characterized. The ATPase was a typical H+-ATPase with similar properties to the Saccharomyces cerevisiae and Zygosaccharomyces rouxii enzymes. It was reacted with antibody (IgG) raised against S. cerevisiae plasma membrane H+-ATPase. The ATPase activity was not changed by adding NaCl and KCl to the assay solutions, but was increased by NH, especially by ammonium sulfate. In vivo stimulation of ATPase activity was observed by the addition of NaCl into the culture medium, as observed in Z. rouxii. No in vivo activation of H+-ATPase by glucose metabolism was observed in C. versatilis cells and the activity was independent of the growth phase, like Z. rouxii and unlike S. cerevisiae cells. 相似文献
84.
Yasuo Kagawa Mami Hiraoka Yukiko Miyashita-Hatano Madoka Shishido-Oki Mamiko Yoshida Susumu Kondou Mika Sugiura Kazumi Sawakami-Kobayashi Masaaki Takahashi Hideji Tajima Masafumi Yohda 《Journal of Bioscience and Bioengineering》2010,110(4):505-508
A low-cost and simple on-site technique for genotyping single nucleotide polymorphisms (SNPs) was developed. The technique is based on allele-specific primer PCR and the recently developed bead arrays in a single tip technique. The performance of the method was verified by genotyping four SNPs that correlate with cardiovascular diseases. 相似文献
85.
Tetsuro Hori Naoki Fukuoka Tetsuya Masuda Yasuo Miyake Hiroyuki Yoshida Akihiko Fujii Yo Shimizu Masanori Ozaki 《Solar Energy Materials & Solar Cells》2011,95(11):3087-3092
Bulk heterojunction solar cells utilizing soluble phthalocyanine derivative, 1,4,8,11,15,18,22,25-octahexylphthalocyanine (C6PcH2) have been investigated. The active layer was fabricated by spin-coating the mixed solution of C6PcH2 and 1-(3-methoxy-carbonyl)-propyl-1-1-phenyl-(6,6)C61 (PCBM). The photovoltaic properties of the solar cell with bulk heterojunction of C6PcH2 and PCBM demonstrated the strong dependence of active layer thickness, and the optimized active layer thickness was clarified to be 120 nm. By inserting MoO3 hole transport buffer layer between the positive electrode and active layer, the FF and energy conversion efficiency were improved to be 0.50 and 3.2%, respectively. The tandem organic thin-film solar cell has also been studied by utilizing active layer materials of C6PcH2 and poly(3-hexylthiophene) and the interlayer of LiF/Al/MoO3 structure, and a high Voc of 1.27 V has been achieved. 相似文献
86.
Yasuo Kakugawa Yutaka Saito Takahisa Matsuda Takeshi Nakajima Mototaka Miyake Gen Iinuma 《International journal of molecular sciences》2013,14(12):23629-23638
To date, few reports focused primarily on detecting colorectal laterally spreading tumors (LSTs) have been published. The aim of this study was to determine the visibility of LSTs on computed tomographic colonography (CTC) compared with that on colonoscopy as a standard. We retrospectively reviewed and matched data on endoscopic and CTC reports in 157 patients (161 LSTs) who received a multidetector CT scan using contrast media immediately after total colonoscopy at the National Cancer Center Hospital in Tokyo, Japan, between December 2005 and August 2010. The results of the total colonoscopy were known at the time of the CTC procedure and reading. Of the 161 LSTs detected on colonoscopy, 138 were observed and matched by CTC (86%). Of the 91 granular type LSTs (LST-Gs), 88 (97%) were observed and matched, while of the 70 non-granular type LSTs (LST-NGs), 50 (71%) were observed and matched by CTC (p < 0.0001). CTC enabled observation of 73% (22/30) of 20–29 mm, 83% (35/42) of 30–39 mm, 88% (49/56) of 40–59 mm, and 97% (32/33) of ≥60 mm tumors. The rate of observed LSTs by CTC was 86% (97% of LST-G, 71% of LST-NG) of the LSTs found during total colonoscopy. 相似文献
87.
Guang-chao Chen Meiyong Liao Masataka Imura Kiyomi Nakajima Yoshimasa Sugimoto Yasuo Koide 《Diamond and Related Materials》2010,19(4):319-323
Strontium titanate (STO) films were directly deposited on Ib (100) single crystal diamond by r.f. magnetron sputtering. The as-deposited STO film was in amorphous state. On the other hand, the crystalline STO film was obtained under the optimized condition of a deposition temperature of 250 °C and a post-annealing temperature of 650 °C. STO/diamond junctions were fabricated on boron-doped homoepitaxial layers grown on p+-type single crystal diamond substrates. Electrical properties of the STO/diamond junction were investigated by changing the surface terminations of diamond with hydrogen or oxygen and the crystallinity of the STO film. It was found that the amorphous STO acted like a semi-insulator on H-diamond surface and that the amorphous STO/O-diamond junction behaved like a Schottky diode. The crystalline STO/O-diamond showed a complex rectifying behavior. The crystalline STO film possessed a higher dielectric constant as compared to that of the amorphous one. 相似文献
88.
Masahiro Osakabe Yasuo Koizumi Taisuke Yonomoto Hiroshige Kumamaru Kanji Tasaka 《Nuclear Engineering and Design》1986,98(1)
High-pressure boiloff experiments in a wide range of bundle powers by using the Two-Phase Flow Test Facility (TPTF) were conducted. Two kinds of boiloff patterns were observed in these experiments. One is the boiloff pattern in a low bundle power, in which the dryout points of rods locate at a certain elevation in the bundle because the mixture level controls the dryout points. The other is the boiloff pattern in a high bundle power, in which the clear mixture level can not be observed and the dryout points of rods locate in a wide range of vertical directions. The vertical scatter of the dryout points is considered to be due to the break of the thin water film on the heater rods under the annular flow pattern.A simple model to predict the slug to annular flow transition in the rod bundle is proposed. In the model, the slug to annular flow transition takes place when the interferences of the water films on the neighboring rods cease. The model appeares to give good predictions of the previous flow transition experiment conducted in a rod bundle. The slug-annular transition below the dryout points was predicted with the present model in the high power boiloff experiments of TPTF. No slug-annular transition below the dryout points is predicted with the present model in the low power boiloff experiments. 相似文献
89.
Y Kajiyama M Tsurumaru H Udagawa K Tsutsumi Y Kinoshita M Ueno H Akiyama 《Canadian Metallurgical Quarterly》1998,45(23):1442-1445
BACKGROUND/AIMS: In the chemotherapy of recurrent gastric cancer with 5-fluorouracil and folinic acid, an interesting phenomenon, the reduction of jaundice and improvement of liver function, was observed. The aim of this study was to investigate the mechanism of this phenomenon, and to confirm a choleretic effect of folinic acid. METHODOLOGY: The bile duct of anesthetized rats was catheterized, and bile was collected. Bile volume and total bile acid production following intraperitoneal administration of folinic acid were determined and compared to those of control rats. RESULTS: Both bile volume and total bile acid production increased following intraabdominal administration of folinic acid. CONCLUSIONS: Folinic acid has a newly described pharmacologic effect of stimulating bile acid-dependent choleresis. It is possible that folinic acid may become a new drug for the treatment of jaundice or for the improvement of overall liver function. 相似文献
90.