The linear plasmid pDHL1 from Debaryomyces hansenii encodes a protein highly homologous to the pGKL1-plasmid DNA polymerase

Both the linear plasmids, pDHL1 (8.4 kb) and pDHL2 (9.2 kb), of Debaryomyces hansenii TK require the presence of a third linear plasmid pDHL3 (15.0 kb) in the same host cell for their replication. A 3.5 kb Bam HI-PstI fragment of pDHL1 strongly hybridized by Southern analysis to the 3.5 kb NcoI-AccI fragment of pDHL2, suggesting the importance of this conserved region in the replication of the two smaller pDHL plasmids. The 4.2 kb pDHL1 fragment containing the above hybridized region was cloned and sequenced. The results showed that the cloned pDHL1 fragment encodes a protein of 1000 amino acid residues, having a strong similarity to the DNA polymerase coded for by ORF1 of the killer plasmid pGKL1 from Kluyveromyces lactis. The catalytic and proof-reading exonuclease domains as well as terminal protein motif were well conserved as in DNA polymerases of pGKL1 and other yeast linear plasmids. Analysis of the cloned fragment further showed that pDHL1 encodes a protein partly similar to the alpha subunit of the K. lactis killer toxin, although killer activity was not known in the DHL system. Analysis of the 5' non-coding region of the two above pDHL1-ORFs reveal the presence of the upstream conserved sequence similar to that found upstream of pGKL1-ORFs. The possible hairpin loop structure was also found just in front of the ATG start codon of the pDHL1-ORFs like pGKL1-ORFs. Thus the cytoplasmic pDHL plasmids were suggested to possess a gene expression system comparable to that of K. lactis plasmids.     

Localization of glycosaminoglycans (GAGs) in pleomorphic adenoma (PA) of salivary glands: an immunohistochemical and histochemical evaluation

The tumor matrix of salivary pleomorphic adenoma (PA) is characteristically rich in glycosaminoglycans (GAGs), which contribute to its complex histoarchitecture. This study evaluated the microscopic localization of various GAGs in 17 PAs, using a panel of anti-GAG monoclonal antibodies and biotinylated hyaluronic acid (HA)-binding protein. Both epithelial and mesenchymal-like tissues were confirmed to contain GAGs. Luminal epithelial cells mostly lacked GAGs, whereas GAGs were seen both in the cytoplasm and cell membrane of non-luminal epithelial cells. In addition, small intercellular accumulations of GAGs were often present in solid epithelial areas, implying the epithelial origin of GAGs. GAGs did not appear to be a main component of the hyaline matrix. The myxoid region was consistently stained for both chondroitin 6-sulfate (CS-6) and HA but variably for chondroitin 4-sulfate (CS-4), dermatan sulfate (DS) and keratan sulfate (KS); heparan sulfate (HS) was not detected. The chondroid region showed increased staining for CS-6 but reduced staining for HA when compared with the myxoid region. In addition, CS-4, DS and KS were seen both in chondroid cells and the territorial matrix, whereas HS was present only in the cells. It is suggested that GAGs in PA are mainly produced by non-luminal cells and influence the proliferation, differentiation, secretory activity and shape of tumor cells, thus contributing to the morphological diversity of this tumor.     

Stability robustness for linear state space models - a Lyapunov mapping approach

Some Pythagorean-like conditions for the existence of a common Lyapunov solution for two given matrices are presented. These conditions are set on the Lyapunov mappings associated with given matrices. The key is the semipositiveness of linear mappings defined on symmetric matrices. From the viewpoint of the existence of a common Lyapunov solution, some known stability robustness results are reviewed and refined.     

日本式公寓——丰富而小的空间

一、日本式公寓的历史 1923年，关东大地震袭击了日本。在关东地区，包括东京和其他外围城市在内的大量建筑和住宅都在地震中倒塌。木结构住宅遭到了破坏性的损害。1924年，“同润会”成立，其目标是建造抗震效果更好的钢筋混凝土建筑。1926年，“同润会”建成了他们的第一栋钢筋混凝土公寓，而他们的最后一个作品完成于1934年。图1是位于代官山的一个同润会公寓。它的建筑面积约为30m^2，包括两个榻榻米房间。     

爱知县淑德大学9号楼

这座建筑是大学的语言教育中心,设有500台计算机、影像和音响系统,主要用于语言教学、留学生教学及国际交流活动。作为建筑“外皮”的性能,设计满足以下三点要求:①充分考虑减少计算机显示器的光反射,营造照度对比小的均质柔和的光环境。②在影像放映时,光环境可渐变调节。③适度的外墙散热功能。教室分别布置在南北两侧,南面的波纹玻璃和合成树脂板材形成双层表皮,中间设计有可调控百叶,能根据日照的入射角度调节;夏季可排热,冬季则可让阳光照射进室内,从而既可获得柔和均质的采光效果又可对热负载进行有效的控制。在建筑北侧满铺的玻璃砖…     

Effects of carbenoxolone Na on acute and chronic gastric ulcer models in experimental animals

Effects of carbenoxolone Na on acute or chronic types of gastric lesions or ulcer models produced in rats, guinea pigs, or dogs were studied. Carbenoxolone Na, given either orally or intraperitoneally, produced a significant inhibition of stress-induced gastric lesions in intact or in pylorus-ligated rats. Acetylsalicylic acid (ASA)-induced or serotonin-induced gastric lesions in rats were also inhibited significantly by pretreatment with the drug. However, carbenoxolone Na did not affect the development of Shay ulceration in rats even though the peptic activity in gastric juices was markedly reduced by the drug. Histamine-induced gastric lesions in guinea pigs were not prevented by pretreatment with carbenoxolone Na. Although carbenoxolone Na, given for 10-20 days, did not promote the healing of stress-induced gastric lesions and acetic acid gastric jlcers in rats, it significantly accelerated the healing of chronic gastric ulcer produced in dogs by 3 weeks' treatment. Carbenoxolone Na prevented the acid back-diffusion caused by ASA without any influence on Na+ efflux in pylorus-ligated rats.     

Real-time monitoring system of tool breakage using Kalman filtering

In this paper, a real-time monitoring system of tool breakage, which measures the cutting torque from the spindle motor current, is described. The system uses an auto-regressive (AR)-model which is adapted for the measured signal using the Kalman filtering technique. The tool breakage is detected by monitoring the residual, that is, the deviation of the observed signal from the estimated value obtained by the AR-model. Additional processing of the residual is introduced to discriminate the signature of tool breakage from the effect caused by the marked change in the cutting condition. To reduce the computation time, the fast calculation algorithm is adopted for estimating the coefficient vector of the AR-model. The monitoring experiment has proven the effectiveness of the system in a milling operation.     

Involvement of heat shock protein 90 in the degradation of mutant insulin receptors by the proteasome

We previously reported three families with type A insulin-resistant syndrome who had mutations, either Asp1179 or Leu1193, in the kinase domain of the insulin receptor. The extreme insulin resistance of these patients was found to be caused by the decreased number of insulin receptors on the cell surface, due to the intracellular rapid degradation (Imamura, T., Takata, Y., Sasaoka, T., Takada, Y., Morioka, H., Haruta, T., Sawa, T., Iwanishi, M., Yang, G. H., Suzuki, Y., Hamada, J., and Kobayashi, M. (1994) J. Biol. Chem. 269, 31019-31027). In the present study, we first examined whether these mutations caused rapid degradation of unprocessed proreceptors, using the exon 13 deleted mutant insulin receptors (DeltaEx13-IR), which were accumulated in the endoplasmic reticulum as unprocessed proreceptors. The addition of Asp1179 or Leu1193 mutation to DeltaEx13-IR caused accelerated degradation of the unprocessed DeltaEx13-IR in the transfected COS-7 cells. Next, we tested whether these mutant receptors were degraded by the proteasome. Treatment with proteasome inhibitors Z-Leu-Leu-Nva-H (MG-115) or Z-Leu-Leu-Leu-H (MG-132) prevented the accelerated degradation of these mutant receptors, resulting in increased amounts of the mutant receptors in the COS-7 cells. Essentially the same results were obtained in the patient's transformed lymphocytes. Finally, we found that these mutant receptors bound to heat shock protein 90 (Hsp90). To determine whether Hsp90 played an important role in the accelerated receptor degradation, we examined the effect of anti-Hsp90 antibody on the mutant receptor degradation. The microinjection of anti-Hsp90 antibody into cells prevented the accelerated degradation of both Asp1179 and Leu1193 mutant insulin receptors. Taken together, these results suggest that Hsp90 is involved in dislocation of the mutant insulin receptors out of the endoplasmic reticulum into the cytosol, where the mutant receptors are degraded by the proteasome.     

Ceramic capillary splices for optical fibres

A simple technique for splicing optical fibres is reported. The technique utilises a self-aligning ceramic capillary with inner dimensions slightly larger than the optical fibre. An average loss of below 0.1 dB is obtained for splices in graded-index and single-mode fibres with a 50 and 10 ?m core diameter, respectively.